AB196556

Anti-FBP1 antibody

Name: Anti-FBP1 antibody (ab196556) | Abcam
Brand: Abcam Limited
SKU: AB196556
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal FBP1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FBP1.

View Alternative Names

FBP, FBP1, FBPase 1, Liver FBPase

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody (AB196556)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody (AB196556)

Immunofluorescence analysis of MCF7 cells, labeling FBP1 (green) with ab196556 at 1/50 dilution. Lower panel shows DAPI for nuclear staining (blue).

Supplier Data

Western blot - Anti-FBP1 antibody (AB196556)

All lanes:

Western blot - Anti-FBP1 antibody (ab196556) at 1/500 dilution

Lane 1:

THP1 cell extract

Lane 2:

Mouse kidney extract

Lane 3:

Mouse liver extract

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FBP1.

P09467

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBP1 also known as fructose-16-bisphosphatase 1 is an enzyme with a molecular mass of approximately 37 kDa. It plays a mechanical role in catalyzing the conversion of fructose-16-bisphosphate to fructose 6-phosphate in the gluconeogenesis pathway. This enzyme expresses primarily in the liver but also in kidneys and muscles reflecting its key role in glucose metabolism.

Biological function summary

The function of FBP1 involves maintaining glucose homeostasis which is essential for energy balance. FBP1 is part of the gluconeogenesis pathway a process that synthesizes glucose from non-carbohydrate sources. This enzyme acts independently and does not usually form part of larger protein complexes but its activity significantly impacts liver metabolism and energy production in low-glucose conditions.

Pathways

FBP1 finds its primary involvement in the gluconeogenesis pathway. This roles complements those of other enzymes such as phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase which also contribute to glucose synthesis. FBP1's activity supports glucose production providing glucose for bloodstream circulation when dietary intake is low.

FBP1's function directly relates to conditions such as fructose 16-bisphosphatase deficiency and has implications in type 2 diabetes. Deficient FBP1 can lead to life-threatening hypoglycemia demonstrating the need for energy regulation by this enzyme. Its regulation intersects with insulin signaling pathways with proteins like insulin receptor substrate (IRS) playing roles in modulating its activity in metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.

See full target information FBP1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 16:8563-8571 PubMed28990097

2017

Fructose‑1,6‑bisphosphatase‑1 decrease may promote carcinogenesis and chemoresistance in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haoran Li,Mengjiao Li,Yangyang Pang,Fei Liu,Dong Sheng,Xi Cheng

Oncology letters 13:3046-3056 PubMed28529559

2017

FBP1 expression is associated with basal-like breast carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Shi,Chunbo Zhao,Haihong Pu,Qingyuan Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FBP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody (AB196556)

Western blot - Anti-FBP1 antibody (AB196556)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise