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Rabbit Recombinant Monoclonal FBP1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant full length protein - Human, Mouse, Rat samples. Cited in 17 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (AB109732), expandable thumbnail
  • Western blot - Anti-FBP1 antibody [EPR4620] (AB109732), expandable thumbnail
  • Western blot - Anti-FBP1 antibody [EPR4620] (AB109732), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-FBP1 antibody [EPR4620] (AB109732), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (AB109732), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Mouse
Tested
Not recommended
Expected
Expected
Rat
Tested
Not recommended
Expected
Expected
Recombinant full length protein - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Recombinant full length protein - Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
10 µg/mL
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/400
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.

Alternative names

FBP, FBP1, FBPase 1, Liver FBPase

Recommended products

Rabbit Recombinant Monoclonal FBP1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant full length protein - Human, Mouse, Rat samples. Cited in 17 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4620
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FBP1 also known as fructose-16-bisphosphatase 1 is an enzyme with a molecular mass of approximately 37 kDa. It plays a mechanical role in catalyzing the conversion of fructose-16-bisphosphate to fructose 6-phosphate in the gluconeogenesis pathway. This enzyme expresses primarily in the liver but also in kidneys and muscles reflecting its key role in glucose metabolism.

Biological function summary

The function of FBP1 involves maintaining glucose homeostasis which is essential for energy balance. FBP1 is part of the gluconeogenesis pathway a process that synthesizes glucose from non-carbohydrate sources. This enzyme acts independently and does not usually form part of larger protein complexes but its activity significantly impacts liver metabolism and energy production in low-glucose conditions.

Pathways

FBP1 finds its primary involvement in the gluconeogenesis pathway. This roles complements those of other enzymes such as phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase which also contribute to glucose synthesis. FBP1's activity supports glucose production providing glucose for bloodstream circulation when dietary intake is low.

Associated diseases and disorders

FBP1's function directly relates to conditions such as fructose 16-bisphosphatase deficiency and has implications in type 2 diabetes. Deficient FBP1 can lead to life-threatening hypoglycemia demonstrating the need for energy regulation by this enzyme. Its regulation intersects with insulin signaling pathways with proteins like insulin receptor substrate (IRS) playing roles in modulating its activity in metabolic disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (ab109732), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (ab109732)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FBP1 with purified ab109732 at 1:50 dilution (7.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-FBP1 antibody [EPR4620] (ab109732), expandable thumbnail

    Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)

    All lanes: Western blot - Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution

    Lane 1: Human kidney lysates at 20 µg

    Lane 2: Mouse liver lysates at 20 µg

    Lane 3: Mouse kidney lysates at 20 µg

    Lane 4: Rat liver lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa

    Observed band size: 36 kDa

  • Western blot - Anti-FBP1 antibody [EPR4620] (ab109732), expandable thumbnail

    Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)

    All lanes: Western blot - Anti-FBP1 antibody [EPR4620] (ab109732) at 1/50000 dilution

    All lanes: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa

    Observed band size: 36 kDa

  • Flow Cytometry (Intracellular) - Anti-FBP1 antibody [EPR4620] (ab109732), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-FBP1 antibody [EPR4620] (ab109732)

    Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling FBP1 with purified ab109732 at 1/400 dilution (1 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (ab109732), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (ab109732)

    ICC/IF image of ab109732 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109732, 10μg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

  • Western blot - Anti-FBP1 antibody [EPR4620] (ab109732), expandable thumbnail

    Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)

    All lanes: Western blot - Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution

    Lane 1: Human stomach lysate at 10 µg

    Lane 2: Human fetal liver lysate at 10 µg

    Lane 3: MCF7 lysate at 10 µg

    Predicted band size: 37 kDa

  • Western blot - Anti-FBP1 antibody [EPR4620] (ab109732), expandable thumbnail

    Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)

    Blocking buffer and concentration: 5% NFDM/TBST. 

    Diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution

    Lane 1: DDDK-tagged FBP1 human full-length recombinant protein at 15 ng

    Lane 2: DDDK-tagged FBP2 human full-length recombinant protein at 15 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa

    Observed band size: 37 kDa

    Exposure time: 5s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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