Skip to main content

Rabbit Recombinant Monoclonal FBP2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (AB248412), expandable thumbnail
  • OI-RD Scanning - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (AB248412), expandable thumbnail
  • Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (AB248412), expandable thumbnail
  • Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (AB248412), expandable thumbnail
  • Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (AB248412), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Not recommended
Tested
Not recommended
Mouse
Not recommended
Predicted
Not recommended
Rat
Not recommended
Predicted
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate.

Additional Targets

FBP1

Alternative names

FBPase 2, Muscle FBPase, FBP2

Recommended products

Rabbit Recombinant Monoclonal FBP2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7435
Purification technique
Affinity purification Protein A
Specificity

The immunogen used for this product shares 93% homology with FBP1. Cross-reactivity with this protein has not been confirmed experimentally.

Dissociation constant
5.17 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248412 is the carrier-free version of Anti-FBP1 + FBP2 antibody [EPR7435] ab131333.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Fructose-16-bisphosphatase (FBP1) and fructose-16-bisphosphatase 2 (FBP2) are enzymes that play an important role in gluconeogenesis. They catalyze the conversion of fructose-16-bisphosphate to fructose-6-phosphate an important metabolic step in glucose production. FBP1 sometimes called liver FBPase is a protein of approximately 37 kDa and it is mainly expressed in the liver kidneys and adipose tissue. FBP2 also known as muscle FBPase is primarily found in muscle tissues. These enzymes share similar enzymatic functions but differ in their regulation and tissue distribution.

Biological function summary

These enzymes help regulate glucose levels in the body. They function as part of a larger enzyme complex involved in energy homeostasis. FBP1 plays a significant role in balancing blood sugar levels during fasting by promoting gluconeogenesis in the liver. FBP2 on the other hand contributes to muscle energy management. The activity of these enzymes is tightly regulated by allosteric inhibitors and activators and they interact with other metabolic enzymes to maintain homeostasis.

Pathways

Fructose-16-bisphosphatase is involved in the gluconeogenesis pathway and is closely linked with the glycolysis pathway. In gluconeogenesis FBP1 and FBP2 convert fructose-16-bisphosphate to fructose-6-phosphate with dihydroxyacetone phosphate as an important relay. They work in tandem with other enzymes like phosphoenolpyruvate carboxykinase to facilitate glucose production. This makes them integral to metabolic pathways that provide glucose during periods of low carbohydrate intake.

Associated diseases and disorders

Dysregulation of FBP1 and FBP2 contributes to metabolic disorders such as type 2 diabetes and hypoglycemia. Overactivity of FBP1 can lead to increased glucose production complicating glucose management in diabetes mellitus. On the other hand FBP2 mutations may impair muscle function and energy utilization. The proteins' imbalances also relate to insulin resistance as their activity influences insulin signaling pathways within metabolic disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (ab248412), expandable thumbnail

    Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (ab248412)

    This data was developed using Anti-FBP1 + FBP2 antibody [EPR7435] ab131333, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] (Anti-FBP1 + FBP2 antibody [EPR7435] ab131333) at 1/1000 dilution

    Lane 1: Fetal kidney lysates at 10 µg

    Lane 2: Human skeletal muscle lysates at 10 µg

    Lane 3: Fetal liver lysates at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 37 kDa

  • OI-RD Scanning - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (ab248412), expandable thumbnail

    OI-RD Scanning - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (ab248412)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (ab248412), expandable thumbnail

    Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (ab248412)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-Lipoamide Dehydrogenase antibody [EPR6635] ab133551).

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST
    Negative control: HL-60, U-937.

    All lanes: Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] (Anti-FBP1 + FBP2 antibody [EPR7435] ab131333) at 1/1000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HL-60 (human Acute Promyelocytic Leukemia promyeloblast) whole cell lysate at 20 µg

    Lane 3: U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 37 kDa

    Exposure time: 10s

  • Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (ab248412), expandable thumbnail

    Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (ab248412)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-Lipoamide Dehydrogenase antibody [EPR6635] ab133551).

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] (Anti-FBP1 + FBP2 antibody [EPR7435] ab131333) at 1/1000 dilution

    All lanes: Human liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 37 kDa

    Exposure time: 3s

  • Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (ab248412), expandable thumbnail

    Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (ab248412)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
    This data was developed using Anti-FBP1 + FBP2 antibody [EPR7435] ab131333, the same antibody clone in a different buffer formulation.

    Lanes 1 - 2: Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] (Anti-FBP1 + FBP2 antibody [EPR7435] ab131333) at 1/10000 dilution

    Lanes 1 - 2: Western blot - Anti-FBP1 + FBP2 antibody [EPR7435] - BSA and Azide free (ab248412)

    Lane 1: C-Myc/DDK-tagged human FBP1 full length recombinant protein at 10 ng

    Lane 2: C-Myc/DDK-tagged human FBP2 full length recombinant protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 37 kDa

    Exposure time: 20s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com