AB131253

Anti-FBP2 antibody [EPR7436]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal FBP2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

FBPase 2, Muscle FBPase, FBP2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP2 antibody [EPR7436] (AB131253)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP2 antibody [EPR7436] (AB131253)

Immunohistochemical analysis of paraffin embedded Human muscle tissue labelling FBP2 with ab131253 antibody at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-FBP2 antibody [EPR7436] (AB131253)

All lanes:

Western blot - Anti-FBP2 antibody [EPR7436] (ab131253) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 10 µg

Lane 2:

Rat muscle tissue lysate at 10 µg

Lane 3:

Mouse muscle tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 37 kDa

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OI-RD Scanning - Anti-FBP2 antibody [EPR7436] (AB131253)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-FBP2 antibody [EPR7436] (AB131253)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7436

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBP2 also known as fructose-16-bisphosphatase 2 is an enzyme that catalyzes the conversion of fructose-16-bisphosphate to fructose 6-phosphate. This reaction is important for the process of gluconeogenesis. FBP2 weighs around 37.6 kDa and is expressed in various tissues including the liver and kidney where it supports energy metabolism by synthesizing glucose from non-carbohydrate precursors.

Biological function summary

The enzyme plays a role in glucose homeostasis by regulating gluconeogenesis. It is a part of the fructose-16-bisphosphatase complex that controls the balance between glycolysis and gluconeogenesis. FBP2 activity helps to maintain blood glucose levels particularly during periods of fasting or intense physical activity. By adjusting the rate of glucose production and consumption the enzyme contributes to metabolic flexibility.

Pathways

FBP2 functions in the regulation of the gluconeogenesis pathway. This pathway is key in maintaining normal blood glucose levels and the enzyme acts in coordination with related enzymes like glucose-6-phosphatase. FBP2 is involved in the balance between glycolysis and gluconeogenesis partnering with enzymes such as phosphofructokinase which perform opposite functions to regulate energy production and consumption. This fine-tuning of energy pathways ensures that cells have a stable supply of glucose adjusting for factors such as nutrient availability and hormonal signals.

FBP2 has connections to metabolic disorders like type 2 diabetes and glycogen storage diseases. Impaired FBP2 activity can disrupt glucose homeostasis leading to dysregulated blood sugar levels characteristic of type 2 diabetes. Additionally the activity of FBP2 can influence the function of other proteins like insulin receptor substrates impacting insulin signaling pathways. Dysfunctions in these pathways can exacerbate insulin resistance and metabolic syndromes emphasizing the significance of FBP2 in maintaining metabolic health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate.

See full target information FBP2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Current research in physiology 3:34-43 PubMed34746818

2020

Effect of post-exercise lactate administration on glycogen repletion and signaling activation in different types of mouse skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Kenya Takahashi,Yu Kitaoka,Yutaka Matsunaga,Hideo Hatta

Experimental & molecular medicine 52:485-496 PubMed32203098

2020

The essential role of fructose-1,6-bisphosphatase 2 enzyme in thermal homeostasis upon cold stress.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun-Jun Park,Hye Rim Jang,Shi-Young Park,Young-Bum Kim,Hui-Young Lee,Cheol Soo Choi

Cell metabolism 31:174-188.e7 PubMed31761563

2019

Fructose-1,6-Bisphosphatase 2 Inhibits Sarcoma Progression by Restraining Mitochondrial Biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Peiwei Huangyang,Fuming Li,Pearl Lee,Itzhak Nissim,Aalim M Weljie,Anthony Mancuso,Bo Li,Brian Keith,Sam S Yoon,M Celeste Simon

Pharmacology 104:173-186 PubMed31280269

2019

MicroRNA-591 Functions as a Tumor Suppressor in Hepatocellular Carcinoma by Lowering Drug Resistance through Inhibition of Far-Upstream Element-Binding Protein 2-Mediated Phosphoinositide 3-Kinase/Akt/Mammalian Target of Rapamycin Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Huili Wang,Yanhua Tang,Dong Yang,Linjing Zheng

PloS one 9:e101315 PubMed24983750

2014

Long-term supranutritional supplementation with selenate decreases hyperglycemia and promotes fatty liver degeneration by inducing hyperinsulinemia in diabetic db/db mice.

Applications

Species

Unspecified reactive species

Chaoqun Wang,Shulin Yang,Ningbo Zhang,Yulian Mu,Hongyan Ren,Yefu Wang,Kui Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FBP2 antibody [EPR7436]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP2 antibody [EPR7436] (AB131253)

Western blot - Anti-FBP2 antibody [EPR7436] (AB131253)

OI-RD Scanning - Anti-FBP2 antibody [EPR7436] (AB131253)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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Product promise