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AB248391

Anti-FBP2 antibody [EPR7436] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal FBP2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

FBPase 2, Muscle FBPase, FBP2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (AB248391)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (AB248391)

This data was developed using ab131253, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human muscle tissue labelling FBP2 with ab131253 antibody at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (AB248391)
  • WB

Unknown

Western blot - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (AB248391)

This data was developed using ab131253, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FBP2 antibody [EPR7436] (<a href='/en-us/products/primary-antibodies/fbp2-antibody-epr7436-ab131253'>ab131253</a>) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 10 µg

Lane 2:

Rat muscle tissue lysate at 10 µg

Lane 3:

Mouse muscle tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 37 kDa

false

OI-RD Scanning - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (AB248391)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (AB248391)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7436

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Primary Antibodies

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Primary Antibodies

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Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab248391 is the carrier-free version of ab131253.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBP2 also known as fructose-16-bisphosphatase 2 is an enzyme that catalyzes the conversion of fructose-16-bisphosphate to fructose 6-phosphate. This reaction is important for the process of gluconeogenesis. FBP2 weighs around 37.6 kDa and is expressed in various tissues including the liver and kidney where it supports energy metabolism by synthesizing glucose from non-carbohydrate precursors.
Biological function summary

The enzyme plays a role in glucose homeostasis by regulating gluconeogenesis. It is a part of the fructose-16-bisphosphatase complex that controls the balance between glycolysis and gluconeogenesis. FBP2 activity helps to maintain blood glucose levels particularly during periods of fasting or intense physical activity. By adjusting the rate of glucose production and consumption the enzyme contributes to metabolic flexibility.

Pathways

FBP2 functions in the regulation of the gluconeogenesis pathway. This pathway is key in maintaining normal blood glucose levels and the enzyme acts in coordination with related enzymes like glucose-6-phosphatase. FBP2 is involved in the balance between glycolysis and gluconeogenesis partnering with enzymes such as phosphofructokinase which perform opposite functions to regulate energy production and consumption. This fine-tuning of energy pathways ensures that cells have a stable supply of glucose adjusting for factors such as nutrient availability and hormonal signals.

FBP2 has connections to metabolic disorders like type 2 diabetes and glycogen storage diseases. Impaired FBP2 activity can disrupt glucose homeostasis leading to dysregulated blood sugar levels characteristic of type 2 diabetes. Additionally the activity of FBP2 can influence the function of other proteins like insulin receptor substrates impacting insulin signaling pathways. Dysfunctions in these pathways can exacerbate insulin resistance and metabolic syndromes emphasizing the significance of FBP2 in maintaining metabolic health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate.
See full target information FBP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 24:457 PubMed36380881

2022

Suppressive effect of YY1-mediated RGS22 regulation on the proliferation, migration and invasion of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shou-Ji Cao,Wan-Li Ge,Ling-Dong Meng,Qun Chen,Yi Miao,Kui-Rong Jiang,Jing-Jing Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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