Rabbit Recombinant Monoclonal FBP2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (AB248391), expandable thumbnail](https://content.abcam.com/products/images/fbp2-antibody-epr7436-bsa-and-azide-free-ab248391--western-blot-img111852.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (AB248391)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (AB248391), expandable thumbnail](https://content.abcam.com/products/images/fbp2-antibody-epr7436-bsa-and-azide-free-ab248391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34960.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (AB248391)")
![OI-RD Scanning - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (AB248391), expandable thumbnail](https://content.abcam.com/products/images/fbp2-antibody-epr7436-bsa-and-azide-free-ab248391--oi-rd-scanning-img139574.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (AB248391)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Mouse | Expected | Not recommended | Tested |
| Rat | Expected | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate.
Alternative names
FBPase 2, Muscle FBPase, FBP2
Recommended products
Rabbit Recombinant Monoclonal FBP2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7436
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 2.9 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248391 is the carrier-free version of Anti-FBP2 antibody [EPR7436] ab131253.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FBP2 also known as fructose-16-bisphosphatase 2 is an enzyme that catalyzes the conversion of fructose-16-bisphosphate to fructose 6-phosphate. This reaction is important for the process of gluconeogenesis. FBP2 weighs around 37.6 kDa and is expressed in various tissues including the liver and kidney where it supports energy metabolism by synthesizing glucose from non-carbohydrate precursors.
- Biological function summary
The enzyme plays a role in glucose homeostasis by regulating gluconeogenesis. It is a part of the fructose-16-bisphosphatase complex that controls the balance between glycolysis and gluconeogenesis. FBP2 activity helps to maintain blood glucose levels particularly during periods of fasting or intense physical activity. By adjusting the rate of glucose production and consumption the enzyme contributes to metabolic flexibility.
- Pathways
FBP2 functions in the regulation of the gluconeogenesis pathway. This pathway is key in maintaining normal blood glucose levels and the enzyme acts in coordination with related enzymes like glucose-6-phosphatase. FBP2 is involved in the balance between glycolysis and gluconeogenesis partnering with enzymes such as phosphofructokinase which perform opposite functions to regulate energy production and consumption. This fine-tuning of energy pathways ensures that cells have a stable supply of glucose adjusting for factors such as nutrient availability and hormonal signals.
- Associated diseases and disorders
FBP2 has connections to metabolic disorders like type 2 diabetes and glycogen storage diseases. Impaired FBP2 activity can disrupt glucose homeostasis leading to dysregulated blood sugar levels characteristic of type 2 diabetes. Additionally the activity of FBP2 can influence the function of other proteins like insulin receptor substrates impacting insulin signaling pathways. Dysfunctions in these pathways can exacerbate insulin resistance and metabolic syndromes emphasizing the significance of FBP2 in maintaining metabolic health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (ab248391), expandable thumbnail](https://content.abcam.com/products/images/fbp2-antibody-epr7436-bsa-and-azide-free-ab248391--western-blot-img111852.jpg "Western blot - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (ab248391)")
Western blot - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (ab248391)
This data was developed using Anti-FBP2 antibody [EPR7436] ab131253, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-FBP2 antibody [EPR7436] (Anti-FBP2 antibody [EPR7436] ab131253) at 1/1000 dilution
Lane 1: Human skeletal muscle lysate at 10 µg
Lane 2: Rat muscle tissue lysate at 10 µg
Lane 3: Mouse muscle tissue lysate at 10 µg
SecondaryAll lanes: HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 37 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (ab248391), expandable thumbnail](https://content.abcam.com/products/images/fbp2-antibody-epr7436-bsa-and-azide-free-ab248391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34960.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (ab248391)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (ab248391)
This data was developed using Anti-FBP2 antibody [EPR7436] ab131253, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human muscle tissue labelling FBP2 with Anti-FBP2 antibody [EPR7436] ab131253 antibody at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (ab248391), expandable thumbnail](https://content.abcam.com/products/images/fbp2-antibody-epr7436-bsa-and-azide-free-ab248391--oi-rd-scanning-img139574.jpg "OI-RD Scanning - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (ab248391)")
OI-RD Scanning - Anti-FBP2 antibody [EPR7436] - BSA and Azide free (ab248391)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com