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AB74023

Anti-Fbx32 antibody

5

(6 Reviews)

|

(34 Publications)

Rabbit Polyclonal FBX32 antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 34 publications.

View Alternative Names

F-box only protein 32, Atrogin-1, Muscle atrophy F-box protein, MAFbx, FBXO32

2 Images
Western blot - Anti-Fbx32 antibody (AB74023)
  • WB

Project5572****

Western blot - Anti-Fbx32 antibody (AB74023)

All lanes:

Western blot - Anti-Fbx32 antibody (ab74023) at 2 µg/mL

Lane 1:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 10 µg

Lane 3:

Rat skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa,90 kDa

false

Exposure time: 15min

Western blot - Anti-Fbx32 antibody (AB74023)
  • WB

CiteAb

Western blot - Anti-Fbx32 antibody (AB74023)

Fbx32 western blot using anti-Fbx32 antibody ab74023. Publication image and figure legend from Liu, S. H., Chiu, C. Y., et al., 2019, Mar Drugs, PubMed 31242648.

ab74023 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab74023 please see the product overview.

Effects of fish oil on the expressions of muscle thick filament protein and atrophy-related proteins in soleus muscles of high-fat diet-fed rats. Rats fed with different experimental diets (standard diet (NC), high-fat diet (HF) in the presence or absence of 5% fish oil (FO) for eight weeks. Protein expressions of myosin heavy chain (MHC), MAFbx/Atrogin-1, Forkhead O (FOXO) 3A, and phosphorylated FOXO3A in the soleus muscles were determined by Western blot. Densitometric analysis for protein levels corrected to each internal control was shown. Results are expressed as mean ± S.D. for each group (n = 6). Values with different letters were significantly different, p < 0.05 (ANOVA with post-hoc Tukey's test).

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is only batch tested in WB and therefore we can only guarantee it for this application. Customers have successfully used this antibody in IHC and ICC but we have not tested it's performance in these applications. If you would like an antibody that is tested in ICC, we would recommend our recombinant antibody ab168372.

Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
See full target information F-box only protein 32

Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 12: PubMed39335522

2024

Increased Myocardial MAO-A, Atrogin-1, and IL-1β Expression in Transgenic Mice with Pancreatic Carcinoma-Benefit of MAO-A Inhibition for Cardiac Cachexia.

Applications

Unspecified application

Species

Unspecified reactive species

Kira Stelter,Annalena Alabssi,Gabriel Alejandro Bonaterra,Hans Schwarzbach,Volker Fendrich,Emily P Slater,Ralf Kinscherf,Wulf Hildebrandt

Journal of cellular and molecular medicine 28:e18096 PubMed38149787

2023

Effects of resistance training on alleviating hypoxia-induced muscle atrophy: Focus on acetylation of FoxO1.

Applications

Unspecified application

Species

Unspecified reactive species

Pengyu Fu,Rongxin Zhu,Weiyang Gao,Lijing Gong

Nutrients 15: PubMed36839161

2023

The Inhibition of Autophagy and Pyroptosis by an Ethanol Extract of Leaf Contributes to the Amelioration of Dexamethasone-Induced Muscle Atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Eunji Park,Hojung Choi,Cao-Sang Truong,Hee-Sook Jun

Frontiers in oncology 12:880787 PubMed35847939

2022

Resistance Training Attenuates Activation of STAT3 and Muscle Atrophy in Tumor-Bearing Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mayra Tardelli de Jesus Testa,Paola Sanches Cella,Poliana Camila Marinello,Fernando Tadeu Trevisan Frajacomo,Camila de Souza Padilha,Patricia Chimin Perandini,Felipe Arruda Moura,José Alberto Duarte,Rubens Cecchini,Flavia Alessandra Guarnier,Rafael Deminice

Journal of cachexia, sarcopenia and muscle 13:1314-1328 PubMed35170238

2022

IFIT2-depleted metastatic oral squamous cell carcinoma cells induce muscle atrophy and cancer cachexia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kuo-Chu Lai,Zi-Xuan Hong,Jyh-Gang Hsieh,Hui-Ju Lee,Muh-Hwa Yang,Chia-Husu Hsieh,Cheng-Han Yang,Yan-Ru Chen

Scientific reports 11:21134 PubMed34702928

2021

E3-ligase knock down revealed differential titin degradation by autopagy and the ubiquitin proteasome system.

Applications

Unspecified application

Species

Unspecified reactive species

Erik Müller,Senem Salcan,Sabine Bongardt,David Monteiro Barbosa,Martina Krüger,Sebastian Kötter

Aging 13:21962-21974 PubMed34537761

2021

Dulaglutide improves muscle function by attenuating inflammation through OPA-1-TLR-9 signaling in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Phyu Phyu Khin,Yeonhee Hong,MyeongHoon Yeon,Dae Ho Lee,Jong Han Lee,Hee-Sook Jun

Aging 12:21446-21468 PubMed33136552

2020

High phosphate induces skeletal muscle atrophy and suppresses myogenic differentiation by increasing oxidative stress and activating Nrf2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Huei Chung,Shu-Ting Liu,Shih-Ming Huang,Donald M Salter,Herng-Sheng Lee,Yu-Juei Hsu

Frontiers in oncology 10:523577 PubMed33102208

2020

Baoyuan Jiedu Decoction Alleviates Cancer-Induced Myotube Atrophy by Regulating Mitochondrial Dynamics Through p38 MAPK/PGC-1α Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Delong Wang,Weiqiao Chen,Qianyu Bi,Xin Zong,Jiazhao Ruan,Xiangjun Yin,Jixin Wang,Honghua Zhang,Xuming Ji

Toxicology and applied pharmacology 402:115119 PubMed32619552

2020

Muscle proteolysis via ubiquitin-proteasome system (UPS) is activated by BthTx-I Lys49 PLA but not by BthTx-II Asp49 PLA and Bothrops jararacussu venom.

Applications

Unspecified application

Species

Unspecified reactive species

Bruno Kenzo-Kagawa,Willians Fernando Vieira,José Carlos Cogo,Maria Alice da Cruz-Höfling
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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