AB92281

Anti-Fbx32 antibody

Name: Anti-Fbx32 antibody (ab92281) | Abcam
Brand: Abcam Limited
SKU: AB92281
Price: 535 USD
Availability: InStock

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(10 Publications)

Goat Polyclonal FBX32 antibody. Suitable for WB and reacts with Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human F-box only protein 32 aa 50-100.

View Alternative Names

F-box only protein 32, Atrogin-1, Muscle atrophy F-box protein, MAFbx, FBXO32

1 Images

Supplier Data

Western blot - Anti-Fbx32 antibody (AB92281)

Lysates in RIPA buffer. Detected by chemiluminescence.

All lanes:

Western blot - Anti-Fbx32 antibody (ab92281) at 0.3 µg/mL

All lanes:

Mouse Skeletal Muscle lysate at 35 µg

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human F-box only protein 32 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q969P5

Specificity

Expected to recognize reported isoform 1 (NP_478136.1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.

See full target information F-box only protein 32

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

PloS one 15:e0241274 PubMed33151986

2020

A model mimicking catabolic inflammatory disease; a controlled randomized study in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Maike Mose,Nikolaj Rittig,Ulla Ramer Mikkelsen,Niels Jessen,Mads Bisgaard Bengtsen,Brit Christensen,Jens Otto Lunde Jørgensen,Niels Møller

Frontiers in physiology 11:203 PubMed32226394

2020

Angiotensin-(1-7) Receptor Mas Deficiency Does Not Exacerbate Cardiac Atrophy Following High-Level Spinal Cord Injury in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Järve,Fatimunnisa Qadri,Mihail Todiras,Shirley Schmolke,Natalia Alenina,Michael Bader

Frontiers in physiology 10:824 PubMed31312144

2019

Influence of Oral Contraceptive Use on Adaptations to Resistance Training.

Applications

Unspecified application

Species

Unspecified reactive species

Line B Dalgaard,Ulrik Dalgas,Jesper L Andersen,Nicklas B Rossen,Andreas Buch Møller,Hans Stødkilde-Jørgensen,Jens Otto Jørgensen,Vuokko Kovanen,Christian Couppé,Henning Langberg,Michael Kjær,Mette Hansen

Frontiers in physiology 10:736 PubMed31258486

2019

Immobilization Decreases FOXO3a Phosphorylation and Increases Autophagy-Related Gene and Protein Expression in Human Skeletal Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Buch Møller,Mikkel Holm Vendelbo,Peter Schjerling,Christian Couppé,Niels Møller,Michael Kjær,Mette Hansen,Niels Jessen

Journal of cancer research and clinical oncology 145:1449-1460 PubMed30968255

2019

Molecular and cellular adaptations to exercise training in skeletal muscle from cancer patients treated with chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Buch Møller,Simon Lønbro,Jean Farup,Thomas Schmidt Voss,Nikolaj Rittig,Jakob Wang,Inger Højris,Ulla Ramer Mikkelsen,Niels Jessen

American journal of physiology. Cell physiology 315:C609-C622 PubMed30110564

2018

Hydrogen sulfide alleviates hyperhomocysteinemia-mediated skeletal muscle atrophy via mitigation of oxidative and endoplasmic reticulum stress injury.

Applications

Unspecified application

Species

Unspecified reactive species

Avisek Majumder,Mahavir Singh,Jyotirmaya Behera,Nicholas T Theilen,Akash K George,Neetu Tyagi,Naira Metreveli,Suresh C Tyagi

Clinical nutrition (Edinburgh, Scotland) 37:2068-2075 PubMed29097038

2017

Impact of the calcium form of β-hydroxy-β-methylbutyrate upon human skeletal muscle protein metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

D J Wilkinson,T Hossain,M C Limb,B E Phillips,J Lund,J P Williams,M S Brook,J Cegielski,A Philp,S Ashcroft,J A Rathmacher,N J Szewczyk,K Smith,P J Atherton

American journal of physiology. Endocrinology and 308:E470-81 PubMed25605643

2015

Resistance exercise-induced S6K1 kinase activity is not inhibited in human skeletal muscle despite prior activation of AMPK by high-intensity interval cycling.

Applications

Unspecified application

Species

Unspecified reactive species

William Apró,Marcus Moberg,D Lee Hamilton,Björn Ekblom,Gerrit van Hall,Hans-Christer Holmberg,Eva Blomstrand

PloS one 9:e102031 PubMed25020061

2014

Fasting increases human skeletal muscle net phenylalanine release and this is associated with decreased mTOR signaling.

Applications

Species

Human

Mikkel Holm Vendelbo,Andreas Buch Møller,Britt Christensen,Birgitte Nellemann,Berthil Frederik Forrest Clasen,K Sreekumaran Nair,Jens Otto Lunde Jørgensen,Niels Jessen,Niels Møller

American journal of physiology. Endocrinology and metabolism 302:E510-21 PubMed22127230

2011

Intake of branched-chain amino acids influences the levels of MAFbx mRNA and MuRF-1 total protein in resting and exercising human muscle.

Applications

Species

Human

Marcus Borgenvik,William Apró,Eva Blomstrand

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fbx32 antibody

Western blot - Anti-Fbx32 antibody (AB92281)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (10)

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