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AB96831

Anti-FbxL12 antibody

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(3 Publications)

Rabbit Polyclonal FbxL12 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human FBXL12 aa 300 to C-terminus.

View Alternative Names

FBL12, FBXL12, F-box/LRR-repeat protein 12, F-box and leucine-rich repeat protein 12, F-box protein FBL12

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FbxL12 antibody (AB96831)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FbxL12 antibody (AB96831)

ab96831, at 1/500 dilution, staining FbxL12 in paraffin-embedded SW480 by Immunohistochemistry.

Western blot - Anti-FbxL12 antibody (AB96831)
  • WB

Unknown

Western blot - Anti-FbxL12 antibody (AB96831)

10% SDS-PAGE

All lanes:

Western blot - Anti-FbxL12 antibody (ab96831) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 37 kDa

false

Western blot - Anti-FbxL12 antibody (AB96831)
  • WB

Unknown

Western blot - Anti-FbxL12 antibody (AB96831)

12% SDS-PAGE

All lanes:

Western blot - Anti-FbxL12 antibody (ab96831) at 1/1000 dilution

All lanes:

NIH-3T3 whole cell lysate at 30 µg

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human FBXL12 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NXK8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the polyubiquitination and proteasomal degradation of CAMK1 leading to disruption of cyclin D1/CDK4 complex assembly which results in G1 cell cycle arrest in lung epithelia.
See full target information FBXL12
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of hematology & oncology 18:27 PubMed40055736

2025

Disruption of the sorcin‒PAX5 protein‒protein interaction induces ferroptosis by promoting the FBXL12-mediated ubiquitination of ALDH1A1 in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yahui Ding,Yongping Bai,Tianyang Chen,Sisi Chen,Wanjing Feng,Shuoqian Ma,Quan Zhang

Nature immunology 20:1381-1392 PubMed31451788

2019

Notch and the pre-TCR coordinate thymocyte proliferation by induction of the SCF subunits Fbxl1 and Fbxl12.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Zhao,Kogulan Yoganathan,LiQi Li,Jan Y Lee,Juan Carlos Zúñiga-Pflücker,Paul E Love

Molecular and cellular biology 36:2182-94 PubMed27215384

2016

SCFFbl12 Increases p21Waf1/Cip1 Expression Level through Atypical Ubiquitin Chain Synthesis.

Applications

WB

Species

Human

Fuminori Tsuruta,Ai Takebe,Kousuke Haratake,Yoshinori Kanemori,Jaehyun Kim,Tomoyuki Endo,Yu Kigoshi,Tomomi Fukuda,Hatsumi Miyahara,Manato Ebina,Tadashi Baba,Tomoki Chiba
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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