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AB272898

Anti-FBXL16 antibody

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Rabbit Polyclonal FBXL16 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human FBXL16.

View Alternative Names

C16orf22, FBL16, FBXL16, F-box/LRR-repeat protein 16, F-box and leucine-rich repeat protein 16

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-FBXL16 antibody (AB272898)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FBXL16 antibody (AB272898)

Paraformaldehyde-fixed MCF7 cells stained for FBXL16 (green) using ab272898 at 1/500 dilution in ICC/IF analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL16 antibody (AB272898)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL16 antibody (AB272898)

Paraffin embedded rat brain tissue stained for FBXL16 using ab272898 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.

Western blot - Anti-FBXL16 antibody (AB272898)
  • WB

Unknown

Western blot - Anti-FBXL16 antibody (AB272898)

All lanes:

Western blot - Anti-FBXL16 antibody (ab272898) at 1/1000 dilution

All lanes:

U-87 MG cell lysate at 30 µg

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human FBXL16. The exact immunogen used to generate this antibody is proprietary information.

Q8N461

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXL16 also known as F-box and leucine-rich repeat protein 16 is a member of the F-box protein family which contains an F-box motif responsible for protein-protein interactions. This protein weighs approximately 46 kDa and plays an important role in tagging proteins for ubiquitin-dependent proteolysis. Scientists find FBXL16 ubiquitously expressed with significant presence in tissues such as the brain heart and testis suggesting its versatile functional significance across different organ systems.
Biological function summary

FBXL16 serves within the cellular context by acting as a component of the Skp1-Cul1-F-box protein (SCF) E3 ubiquitin ligase complex directing specific substrate proteins for ubiquitination. By tagging these substrates FBXL16 contributes to regulated protein turnover modulating pathways necessary for various cellular activities such as cell cycle control and signaling. The efficiency of these cellular processes heavily depends on FBXL16's proper functioning within this complex.

Pathways

FBXL16 interacts with signaling cascades such as the cell cycle regulation pathway and the ubiquitin-proteasome system. These pathways ensure precise control of cell division and protein turnover. Within these pathways FBXL16 works alongside proteins like Skp2 and Cullin1 that form part of the SCF complexes ensuring selective and appropriate degradation of various cellular proteins.

FBXL16 shows links to cancer and neurodegenerative diseases. Its role in regulating cell cycle and protein degradation implicates FBXL16 in tumor progression when dysregulated. Further in neurodegenerative disorders FBXL16 might interact with proteins such as Parkin involved in ubiquitination suggesting its influence on neuronal health. Understanding FBXL16's role in these contexts can offer insights into potential therapeutic strategies targeting its pathway interactions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
See full target information FBXL16
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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