Rabbit Polyclonal FBXL17 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FBXL17.
Images
Publications
Filter by
- Journal of cell science 130:3578-35872017Lipopolysaccharide modulates p300 and Sirt1 to promote PRMT1 stability via an SCF-recognized acetyldegron.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYandong Lai,Jin Li,Xiuying Li,Chunbin ZouPubMed 28883095
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human FBXL17. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UF56
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Substrate-recognition component of the SCF(FBXL17) E3 ubiquitin ligase complex, a key component of a quality control pathway required to ensure functional dimerization of BTB domain-containing proteins (dimerization quality control, DQC) (PubMed:30190310). FBXL17 specifically recognizes and binds a conserved degron of non-consecutive residues present at the interface of BTB dimers of aberrant composition: aberrant BTB dimer are then ubiquitinated by the SCF(FBXL17) complex and degraded by the proteasome (PubMed:30190310). The ability of the SCF(FBXL17) complex to eliminate compromised BTB dimers is required for the differentiation and survival of neural crest and neuronal cells (By similarity). The SCF(FBXL17) complex mediates ubiquitination and degradation of BACH1 (PubMed:24035498, PubMed:30190310). The SCF(FBXL17) complex is also involved in the regulation of the hedgehog/smoothened (Hh) signaling pathway by mediating the ubiquitination and degradation of SUFU, allowing the release of GLI1 from SUFU for proper Hh signal transduction (PubMed:27234298). The SCF(FBXL17) complex mediates ubiquitination and degradation of PRMT1 (By similarity).
Alternative names
FBL17, FBX13, FBXO13, FBXL17, F-box/LRR-repeat protein 17, F-box and leucine-rich repeat protein 17, F-box only protein 13
Recommended products
Rabbit Polyclonal FBXL17 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FBXL17.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human FBXL17. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UF56
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab111683 is purified by antigen-affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FBXL17 also known as F-box/LRR-repeat protein 17 functions as a member of the F-box protein family. These proteins form part of the SCF (SKP1-CUL1-F-box protein) complex a type of E3 ubiquitin ligase involved in protein degradation. FBXL17 has a molecular mass of approximately 49 kDa. It is expressed in various tissues with notable presence in the liver lung and kidney. By interacting with SCF FBXL17 plays a role in targeting specific substrates for ubiquitination which can regulate their stability and function.
- Biological function summary
FBXL17 influences diverse processes through its role in protein ubiquitination. As a component of the SCF complex it regulates cell cycle progression and cell signaling by mediating the degradation of proteins involved in these pathways. Through protein interaction FBXL17 modulates cellular responses and assists in maintaining cellular homeostasis. It does this by recognizing and binding to phosphodegrons on substrates therefore determining their ubiquitination.
- Pathways
FBXL17 interfaces with the cell cycle and signal transduction pathways. It is involved in regulating proteins like cyclins and cyclin-dependent kinases which are fundamental for cell cycle control. Additionally FBXL17 participates in the Wnt/β-catenin signaling pathway. By influencing the stability of proteins like β-catenin FBXL17 can affect gene expression and cellular activities related to these pathways.
- Associated diseases and disorders
FBXL17's dysregulation or altered expression may have connections to cancer and neurodegenerative diseases. In cancer FBXL17 can influence tumorigenesis by altering the degradation of oncoproteins and tumor suppressor genes. Its role in regulating the Wnt/β-catenin pathway associates it with disorders like colorectal cancer. FBXL17's interactions with other ubiquitin ligases such as FBXW7 may contribute to understanding its precise role in disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-FBXL17 antibody (ab111683)
10% SDS-PAGE.
All lanes: Western blot - Anti-FBXL17 antibody (ab111683) at 1/1000 dilution
All lanes: MCF7 whole cell lysate at 30 µg
Predicted band size: 39 kDa, 40 kDa, 52 kDa, 76 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL17 antibody (ab111683)
ab111683 at 1/500 dilution staining FBXL17 in paraffin-embedded U373 xenograft tissue by Immunohistochemistry.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com