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AB172961

Anti-FBXL19 antibody [EPR11957]

4

(2 Reviews)

|

(4 Publications)

Rabbit Recombinant Monoclonal FBXL19 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications.

View Alternative Names

FBL19, FBXL19, F-box/LRR-repeat protein 19, F-box and leucine-rich repeat protein 19

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-FBXL19 antibody [EPR11957] (AB172961)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FBXL19 antibody [EPR11957] (AB172961)

Immunofluorescence analysis of A549 cells labeling FBXL19 with ab172961 at 1/50 dilution.

Western blot - Anti-FBXL19 antibody [EPR11957] (AB172961)
  • WB

Supplier Data

Western blot - Anti-FBXL19 antibody [EPR11957] (AB172961)

All lanes:

Western blot - Anti-FBXL19 antibody [EPR11957] (ab172961) at 1/1000 dilution

Lane 1:

HT1080 lysate at 10 µg

Lane 2:

A549 lysate at 10 µg

Lane 3:

Mouse testis lysate at 10 µg

Predicted band size: 76 kDa

false

  • Carrier free

    Anti-FBXL19 antibody [EPR11957] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11957

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXL19 also known as F-box and leucine-rich repeat protein 19 functions as a subunit of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. This complex plays a vital role in targeting cellular proteins for ubiquitination and proteasomal degradation. The molecular mass of FBXL19 is approximately 74 kDa. FBXL19 can be found in several tissues with notable expression in the brain and reproductive organs. It also locates within the nucleus of cells where it interacts with other components of the ubiquitination machinery.
Biological function summary

FBXL19 influences the regulation of protein stability and turnover through its participation in the SCF complex. This complex involving SKP1 CUL1 and other F-box proteins mediates ubiquitination by recognizing specific phosphorylated substrates. FBXL19 facilitates the recognition of these substrates impacting cellular processes such as cell cycle progression signal transduction and transcription regulation. The precise action of FBXL19 within these complexes highlights its role in maintaining cellular homeostasis by ensuring timely protein degradation.

Pathways

FBXL19 is integral to the protein degradation pathway and the regulation of the cell cycle. The protein interacts with the ubiquitin-proteasome system to modulate the levels of cell cycle regulators maintaining proper cellular division and function. FBXL19 also shows interconnection with proteins such as SKP2 in the regulatory pathway of cell cycle highlighting its involvement in pathways controlling cell proliferation and differentiation.

FBXL19 has links to cancer and neurodegenerative diseases. Its role in ubiquitin-mediated proteolysis affects tumor suppressor proteins and oncogenes which can contribute to oncogenesis. Dysregulation of FBXL19 activity may also impact proteins associated with neurodegenerative disorders such as tau or amyloid precursor protein suggesting a potential involvement in pathologies like Alzheimer's disease. The connection between FBXL19 these proteins and related pathways emphasizes its importance in understanding molecular mechanisms of disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex that plays a role in different processes including cell migration, cell proliferation or cytoskeletal reorganization (PubMed : 24684802, PubMed : 29522376). Mediates RHOA ubiquitination and degradation in a ERK2-dependent manner (PubMed : 23871831). Induces RAC1 and RAC3 degradation by the proteasome system and thereby regulates TGFB1-induced E-cadherin down-regulation and cell migration (PubMed : 23512198, PubMed : 24684802). Mediates also ubiquitination and degradation of IL-33-induced receptor IL1RL1 and subsequently blocks IL-33-mediated apoptosis (By similarity). Within the nucleus, binds to DNA containing unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides (PubMed : 29276034). Recruits CDK-mediator to chromatin and targets CDK8 to promoters of silent developmental genes leading to induction of these genes during cell differentiation. In addition, plays a critical role in the recruitment of RNF20 to histone H2B leading to H2B mono-ubiquitination (By similarity).
See full target information FBXL19
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of cardiothoracic surgery 18:92 PubMed36964598

2023

Protective role of FBXL19 in Streptococcus pneumoniae-induced lung injury in pneumonia immature mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Chen,Bijuan Zheng,Zhiwei Zhang,Zhiyong Huang

Cancer cell international 20:589 PubMed33298070

2020

ETS1-activated SNHG10 exerts oncogenic functions in glioma via targeting miR-532-3p/FBXL19 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lide Jin,Shengquan Huang,Congjin Guan,Shun Chang

Genes & development 33:1367-1380 PubMed31488578

2019

miR-26 suppresses adipocyte progenitor differentiation and fat production by targeting .

Applications

Unspecified application

Species

Unspecified reactive species

Asha Acharya,Daniel C Berry,He Zhang,Yuwei Jiang,Benjamin T Jones,Robert E Hammer,Jonathan M Graff,Joshua T Mendell

Nature methods 15:909-912 PubMed30377371

2018

A high-throughput pipeline for validation of antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof Sikorski,Adi Mehta,Marit Inngjerdingen,Flourina Thakor,Simon Kling,Tomas Kalina,Tuula A Nyman,Maria Ekman Stensland,Wei Zhou,Gustavo A de Souza,Lars Holden,Jan Stuchly,Markus Templin,Fridtjof Lund-Johansen
View all publications
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Product promise

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