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AB249735

Anti-FBXL19 antibody [EPR11957] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal FBXL19 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

FBL19, FBXL19, F-box/LRR-repeat protein 19, F-box and leucine-rich repeat protein 19

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-FBXL19 antibody [EPR11957] - BSA and Azide free (AB249735)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FBXL19 antibody [EPR11957] - BSA and Azide free (AB249735)

This data was developed using ab172961, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of A549 cells labeling FBXL19 with ab172961 at 1/50 dilution.

Western blot - Anti-FBXL19 antibody [EPR11957] - BSA and Azide free (AB249735)
  • WB

Supplier Data

Western blot - Anti-FBXL19 antibody [EPR11957] - BSA and Azide free (AB249735)

This data was developed using ab172961, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FBXL19 antibody [EPR11957] (<a href='/en-us/products/primary-antibodies/fbxl19-antibody-epr11957-ab172961'>ab172961</a>) at 1/1000 dilution

Lane 1:

HT1080 lysate at 10 µg

Lane 2:

A549 lysate at 10 µg

Lane 3:

Mouse testis lysate at 10 µg

Predicted band size: 76 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11957

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab249735 is the carrier-free version of ab172961.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXL19 also known as F-box and leucine-rich repeat protein 19 functions as a subunit of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. This complex plays a vital role in targeting cellular proteins for ubiquitination and proteasomal degradation. The molecular mass of FBXL19 is approximately 74 kDa. FBXL19 can be found in several tissues with notable expression in the brain and reproductive organs. It also locates within the nucleus of cells where it interacts with other components of the ubiquitination machinery.
Biological function summary

FBXL19 influences the regulation of protein stability and turnover through its participation in the SCF complex. This complex involving SKP1 CUL1 and other F-box proteins mediates ubiquitination by recognizing specific phosphorylated substrates. FBXL19 facilitates the recognition of these substrates impacting cellular processes such as cell cycle progression signal transduction and transcription regulation. The precise action of FBXL19 within these complexes highlights its role in maintaining cellular homeostasis by ensuring timely protein degradation.

Pathways

FBXL19 is integral to the protein degradation pathway and the regulation of the cell cycle. The protein interacts with the ubiquitin-proteasome system to modulate the levels of cell cycle regulators maintaining proper cellular division and function. FBXL19 also shows interconnection with proteins such as SKP2 in the regulatory pathway of cell cycle highlighting its involvement in pathways controlling cell proliferation and differentiation.

FBXL19 has links to cancer and neurodegenerative diseases. Its role in ubiquitin-mediated proteolysis affects tumor suppressor proteins and oncogenes which can contribute to oncogenesis. Dysregulation of FBXL19 activity may also impact proteins associated with neurodegenerative disorders such as tau or amyloid precursor protein suggesting a potential involvement in pathologies like Alzheimer's disease. The connection between FBXL19 these proteins and related pathways emphasizes its importance in understanding molecular mechanisms of disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex that plays a role in different processes including cell migration, cell proliferation or cytoskeletal reorganization (PubMed : 24684802, PubMed : 29522376). Mediates RHOA ubiquitination and degradation in a ERK2-dependent manner (PubMed : 23871831). Induces RAC1 and RAC3 degradation by the proteasome system and thereby regulates TGFB1-induced E-cadherin down-regulation and cell migration (PubMed : 23512198, PubMed : 24684802). Mediates also ubiquitination and degradation of IL-33-induced receptor IL1RL1 and subsequently blocks IL-33-mediated apoptosis (By similarity). Within the nucleus, binds to DNA containing unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides (PubMed : 29276034). Recruits CDK-mediator to chromatin and targets CDK8 to promoters of silent developmental genes leading to induction of these genes during cell differentiation. In addition, plays a critical role in the recruitment of RNF20 to histone H2B leading to H2B mono-ubiquitination (By similarity).
See full target information FBXL19
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan
View all publications
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Product promise

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