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AB222894

Anti-FBXL21/FBL3 antibody

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(1 Publication)

Rabbit Polyclonal FBXL21/FBL3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FBXL21P aa 50-150.

View Alternative Names

FBL21, FBL3, FBXL21, FBXL3B, FBXL3P, FBXL21P, Putative F-box/LRR-repeat protein 21, F-box and leucine-rich repeat protein 21, F-box and leucine-rich repeat protein 3B, F-box/LRR-repeat protein 3B

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-FBXL21/FBL3 antibody (AB222894)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FBXL21/FBL3 antibody (AB222894)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for FBXL21/FBL3 (green) using ab222894 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L) .

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL21/FBL3 antibody (AB222894)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL21/FBL3 antibody (AB222894)

Paraffin-embedded human lung cancer tissue stained for FBXL21/FBL3 using ab222894 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FBXL21P aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q9UKT6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXL21 also known as FBL3 is a protein that functions as an E3 ubiquitin ligase which means it is involved in tagging proteins for degradation by the proteasome. FBXL21 belongs to the F-box protein family and contains an F-box domain that allows it to form part of a larger SCF (SKP1-Cullin-F-box) complex. This protein has a molecular mass of approximately 60 kDa. FBXL21 expresses in various tissues including the heart brain and skeletal muscle indicating its vital mechanical role across different physiological systems.
Biological function summary

FBXL21 plays a significant role in regulating circadian rhythms by modulating the stability of core clock proteins. It forms a complex with other proteins such as SKP1 and Cullin-1 to selectively ubiquitinate and degrade specific substrates. Its function is important in maintaining the balance between tau phosphorylation and degradation impacting the internal biological clock. Alterations in this process can significantly affect the organism's ability to adapt to the changes in light and dark cycles.

Pathways

FBXL21 is involved in the circadian pathway regulating the oscillation of clock proteins such as CRY1 and CRY2 which control circadian stability. The protein interacts with pathways related to protein degradation and regulation of the protein levels ensuring timely degradation of phosphorylated clock proteins. FBXL21's interaction with the SCF complex components like SKP1 and Cullin-1 positions it as an essential player in these pathways coordinating the ubiquitin-proteasome system's role in maintaining cellular homeostasis.

FBXL21's dysregulation relates to sleep disorders and potential links to neurodegenerative diseases like Alzheimer's. Disruptions in circadian rhythm proteins like CRY1 and CRY2 can result in altered sleep patterns and cognitive difficulties. Additionally improper FBXL21 function affects tau protein levels which links it to neurodegeneration and diseases associated with tauopathies. Understanding FBXL21's role can contribute to insights into managing circadian-based disorders and neurodegenerative conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-recognition component of the SCF(FBXL21) E3 ubiquitin ligase complex involved in circadian rhythm function. Plays a key role in the maintenance of both the speed and the robustness of the circadian clock oscillation. The SCF(FBXL21) complex mainly acts in the cytosol and mediates ubiquitination of CRY proteins (CRY1 and CRY2), leading to CRY proteins stabilization. The SCF(FBXL21) complex counteracts the activity of the SCF(FBXL3) complex and protects CRY proteins from degradation. Involved in the hypothalamic suprachiasmatic nucleus (SCN) clock regulating temporal organization of the daily activities (By similarity).
See full target information FBXL21P
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 28:268-281.e4 PubMed29937374

2018

Autophagy Regulates the Liver Clock and Glucose Metabolism by Degrading CRY1.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam Toledo,Ana Batista-Gonzalez,Emilio Merheb,Marie Louise Aoun,Elena Tarabra,Daorong Feng,Jaakko Sarparanta,Paola Merlo,Francesco Botrè,Gary J Schwartz,Jeffrey E Pessin,Rajat Singh
View all publications
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Product promise

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