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AB223059

Anti-FBXL22 antibody

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(1 Publication)

Rabbit Polyclonal FBXL22 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FBXL22 aa 100 to C-terminus.

View Alternative Names

F-box and leucine-rich protein 22, FBXL22

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-FBXL22 antibody (AB223059)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FBXL22 antibody (AB223059)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FBXL22 (green) using ab223059 at 1/100 dilution in ICC/IF. Secondary : Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-FBXL22 antibody (AB223059)
  • WB

Supplier Data

Western blot - Anti-FBXL22 antibody (AB223059)

All lanes:

Western blot - Anti-FBXL22 antibody (ab223059) at 1/1000 dilution

Lane 1:

Mouse brain lysate

Lane 2:

Human placenta lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG at 1/50000 dilution

Predicted band size: 27 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL22 antibody (AB223059)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL22 antibody (AB223059)

Paraffin embedded human pancreatic cancer tissue stained for FBXL22 with ab223059 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human FBXL22 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6P050

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Complementary Products

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AB67232

Anti-FBXL20 antibody

Western blot - Anti-FBXL20 antibody (AB67232)

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Primary Antibodies

AB17018

Anti-FBXL2 antibody

Western blot - Anti-FBXL2 antibody (AB17018)

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Primary Antibodies

AB230522

Anti-CASC4 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASC4 antibody - C-terminal (AB230522)

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Primary Antibodies

AB184772

Anti-URG11 antibody

Immunocytochemistry/ Immunofluorescence - Anti-URG11 antibody (AB184772)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity>95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXL22 also known as F-box and leucine-rich repeat protein 22 is a part of the F-box protein family characterized by an F-box motif. This protein weighs approximately 47 kDa. It is a component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which is involved in tagging proteins for degradation by the proteasome. FBXL22 exhibits expression in various tissues with higher levels observed in the testis and brain. It serves as a substrate recognition component targeting specific proteins for ubiquitination facilitating protein turnover and regulation.
Biological function summary

The role of FBXL22 spans beyond proteasomal degradation. It participates in regulating cell cycle progression and DNA damage response impacting cellular homeostasis. As a part of the SCF complex FBXL22 interacts with SKP1 and CUL1 to form a functional E3 ligase which is important in maintaining protein quality control. This function helps in eliminating misfolded or damaged proteins ensuring cellular integrity and defense against stress conditions.

Pathways

FBXL22 acts within the context of the ubiquitin-proteasome system. It plays a role in pathways such as protein quality control and DNA repair. In these pathways FBXL22 associates with proteins like TP53 and MDM2 influencing cellular responses to DNA damage and stress. Its activity impacts the balance between cell survival and apoptosis positioning it as a modulator within cellular stress response pathways.

FBXL22 has implications in cancer and neurodegenerative diseases. Aberrant expression or mutations in FBXL22 can lead to unregulated protein degradation contributing to tumorigenesis. In cancer FBXL22 might interact with proteins such as p53 affecting its stability and function therefore impacting cell cycle checkpoints. Moreover in neurodegenerative conditions altered FBXL22 activity may contribute to the accumulation of damaged proteins exacerbating disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Promotes ubiquitination of sarcomeric proteins alpha-actinin-2 (ACTN2) and filamin-C (FLNC).
See full target information FBXL22
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:5015-5033 PubMed40860180

2025

Citrus Pectin Supplementation Alleviated Hepatic Lipid Accumulation through Gut Microbiota Indole Lactic Acid Promoting Hepatic Bile Acid Synthesis and Excretion.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijun Pan,Xinyuan Jin,Qing Li,Yuqing Zhou,Yupeng Zeng,Xin Wang,Yufeng Jin,Yu Chen,Dan Li,Wenhua Ling
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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