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AB115515

Anti-FBXL3 antibody

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(1 Publication)

Goat Polyclonal FBXL3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FBXL3 aa 1-50.

View Alternative Names

FBL3A, FBXL3A, FBXL3, F-box/LRR-repeat protein 3, F-box and leucine-rich repeat protein 3A, F-box/LRR-repeat protein 3A

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL3 antibody (AB115515)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL3 antibody (AB115515)

ab115515, at 5μg/ml staining FBXL3 in formalin-fixed, paraffin-embedded Human thyroid tissue by Immunohistochemistry, using a biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Western blot - Anti-FBXL3 antibody (AB115515)
  • WB

Unknown

Western blot - Anti-FBXL3 antibody (AB115515)

Primary incubation : 1 hour

All lanes:

Western blot - Anti-FBXL3 antibody (ab115515) at 1 µg/mL

All lanes:

Human adipose lysate (in RIPA buffer) at 35 µg

Predicted band size: 49 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human FBXL3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9UKT7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXL3 also known as F-box and leucine-rich repeat protein 3 acts as part of the protein complex responsible for targeted degradation of other proteins. It has a molecular mass of approximately 46 kDa and plays its role mainly in the nucleus. Researchers have found FBXL3 expressed in multiple tissues including the brain indicating its widespread biological importance. This protein forms part of the Skp Cullin F-box containing complex (SCF complex) which functions in regulating the degradation of proteins via ubiquitination.
Biological function summary

FBXL3 participates in the regulation of circadian rhythms controlling the degradation of specific clock proteins like CRY1 and CRY2. These proteins along with FBXL3 form part of the SCF complex. By targeting CRY proteins for degradation FBXL3 influences the stability and timing of the molecular clock. This role underlines FBXL3's importance in maintaining daily physiological rhythms that affect behavior and biologic processes.

Pathways

FBXL3 integrates into the ubiquitin-proteasome system and the circadian clock pathway. Its interaction with CRY1 and CRY2 proteins highlights its role in circadian rhythm regulation. Additionally FBXL3's function in these pathways affects proteins such as PER proteins which are also relevant for the correct functioning of the biological clock. This pathway regulation is essential for synchronization with the day-night cycle.

Defects in FBXL3 function associate with circadian rhythm sleep disorders and certain mood disorders like bipolar disorder. Dysregulation of CRY proteins due to improper FBXL3 function can affect sleep-wake cycles and lead to these conditions. Furthermore FBXL3 interactions with proteins like CRY1 indicate potential impacts on disorders that relate to disrupted circadian biology influencing both physiological and psychological health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-recognition component of the SCF(FBXL3) E3 ubiquitin ligase complex involved in circadian rhythm function. Plays a key role in the maintenance of both the speed and the robustness of the circadian clock oscillation (PubMed : 17463251, PubMed : 23452855, PubMed : 27565346). The SCF(FBXL3) complex mainly acts in the nucleus and mediates ubiquitination and subsequent degradation of CRY1 and CRY2 (PubMed : 17463251, PubMed : 23452855, PubMed : 27565346). Activity of the SCF(FBXL3) complex is counteracted by the SCF(FBXL21) complex (PubMed : 23452855).
See full target information FBXL3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 29:1520-1536 PubMed32337552

2020

A rare genomic duplication in 2p14 underlies autosomal dominant hearing loss DFNA58.

Applications

Unspecified application

Species

Unspecified reactive species

Karina Lezirovitz,Gleiciele A Vieira-Silva,Ana C Batissoco,Débora Levy,Joao P Kitajima,Alix Trouillet,Ellen Ouyang,Navid Zebarjadi,Juliana Sampaio-Silva,Vinicius Pedroso-Campos,Larissa R Nascimento,Cindy Y Sonoda,Vinícius M Borges,Laura G Vasconcelos,Roberto M O Beck,Signe S Grasel,Daniel J Jagger,Nicolas Grillet,Ricardo F Bento,Regina C Mingroni-Netto,Jeanne Oiticica
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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