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AB96645

Anti-FBXL3 antibody

5

(1 Review)

|

(5 Publications)

Rabbit Polyclonal FBXL3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FBXL3 aa 1-300.

View Alternative Names

FBL3A, FBXL3A, FBXL3, F-box/LRR-repeat protein 3, F-box and leucine-rich repeat protein 3A, F-box/LRR-repeat protein 3A

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-FBXL3 antibody (AB96645)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FBXL3 antibody (AB96645)

ab96645, at a 1/200 dilution, staining FBXL3 in paraformaldehyde fixed A431 by Immunofluorescence analysis. Lower image merged with DNA probe.

Western blot - Anti-FBXL3 antibody (AB96645)
  • WB

Unknown

Western blot - Anti-FBXL3 antibody (AB96645)

10% SDS PAGE

All lanes:

Western blot - Anti-FBXL3 antibody (ab96645) at 1/1000 dilution

All lanes:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 49 kDa

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Western blot - Anti-FBXL3 antibody (AB96645)
  • WB

Supplier Data

Western blot - Anti-FBXL3 antibody (AB96645)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-FBXL3 antibody (ab96645) at 1/500 dilution

All lanes:

C2C12 whole cell lysate at 30 µg

Predicted band size: 49 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FBXL3 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9UKT7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXL3 also known as F-box and leucine-rich repeat protein 3 acts as part of the protein complex responsible for targeted degradation of other proteins. It has a molecular mass of approximately 46 kDa and plays its role mainly in the nucleus. Researchers have found FBXL3 expressed in multiple tissues including the brain indicating its widespread biological importance. This protein forms part of the Skp Cullin F-box containing complex (SCF complex) which functions in regulating the degradation of proteins via ubiquitination.
Biological function summary

FBXL3 participates in the regulation of circadian rhythms controlling the degradation of specific clock proteins like CRY1 and CRY2. These proteins along with FBXL3 form part of the SCF complex. By targeting CRY proteins for degradation FBXL3 influences the stability and timing of the molecular clock. This role underlines FBXL3's importance in maintaining daily physiological rhythms that affect behavior and biologic processes.

Pathways

FBXL3 integrates into the ubiquitin-proteasome system and the circadian clock pathway. Its interaction with CRY1 and CRY2 proteins highlights its role in circadian rhythm regulation. Additionally FBXL3's function in these pathways affects proteins such as PER proteins which are also relevant for the correct functioning of the biological clock. This pathway regulation is essential for synchronization with the day-night cycle.

Defects in FBXL3 function associate with circadian rhythm sleep disorders and certain mood disorders like bipolar disorder. Dysregulation of CRY proteins due to improper FBXL3 function can affect sleep-wake cycles and lead to these conditions. Furthermore FBXL3 interactions with proteins like CRY1 indicate potential impacts on disorders that relate to disrupted circadian biology influencing both physiological and psychological health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-recognition component of the SCF(FBXL3) E3 ubiquitin ligase complex involved in circadian rhythm function. Plays a key role in the maintenance of both the speed and the robustness of the circadian clock oscillation (PubMed : 17463251, PubMed : 23452855, PubMed : 27565346). The SCF(FBXL3) complex mainly acts in the nucleus and mediates ubiquitination and subsequent degradation of CRY1 and CRY2 (PubMed : 17463251, PubMed : 23452855, PubMed : 27565346). Activity of the SCF(FBXL3) complex is counteracted by the SCF(FBXL21) complex (PubMed : 23452855).
See full target information FBXL3
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Genes to cells : devoted to molecular & cellular mechanisms 29:1012-1025 PubMed39357875

2024

Regular exercise suppresses steatosis-associated liver cancer development by degrading E2F1 and c-Myc via circadian gene upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Vu Thuong Huyen,Kanae Echizen,Ryota Yamagishi,Miho Kumagai,Yoshiki Nonaka,Takahiro Kodama,Tatsuya Ando,Megumu Yano,Naoki Takada,Masaki Takasugi,Fumitaka Kamachi,Naoko Ohtani

Clinical and translational medicine 13:e1443 PubMed37837399

2023

Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-box -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Peng Xia,Zhangshuo Yang,Jie Liu,Yimin Zhu,Zan Huang,Zhonglin Zhang,Yufeng Yuan

Neurobiology of disease 137:104697 PubMed31783119

2019

Druggable genome screen identifies new regulators of the abundance and toxicity of ATXN3, the Spinocerebellar Ataxia type 3 disease protein.

Applications

Unspecified application

Species

Unspecified reactive species

Naila S Ashraf,Joanna R Sutton,Yemen Yang,Bedri Ranxhi,Kozeta Libohova,Emily D Shaw,Anna J Barget,Sokol V Todi,Henry L Paulson,Maria do Carmo Costa

Pathology, research and practice 215:358-365 PubMed30594330

2018

FBXL3 is regulated by miRNA-4735-3p and suppresses cell proliferation and migration in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dazhong Wang,Xin Han,Chan Li,Weijun Bai

Cell metabolism 28:268-281.e4 PubMed29937374

2018

Autophagy Regulates the Liver Clock and Glucose Metabolism by Degrading CRY1.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam Toledo,Ana Batista-Gonzalez,Emilio Merheb,Marie Louise Aoun,Elena Tarabra,Daorong Feng,Jaakko Sarparanta,Paola Merlo,Francesco Botrè,Gary J Schwartz,Jeffrey E Pessin,Rajat Singh
View all publications
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Product promise

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