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AB181801

Anti-FBXO11 antibody

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(8 Publications)

Rabbit Polyclonal FBXO11 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human FBXO11.

View Alternative Names

FBX11, PRMT9, VIT1, UG063H01, FBXO11, F-box only protein 11, Protein arginine N-methyltransferase 9, Vitiligo-associated protein 1, VIT-1

2 Images
Western blot - Anti-FBXO11 antibody (AB181801)
  • WB

Supplier Data

Western blot - Anti-FBXO11 antibody (AB181801)

All lanes:

Western blot - Anti-FBXO11 antibody (ab181801) at 1/500 dilution

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

HeLa cell lysate at 7 µg

Predicted band size: 104 kDa

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Immunocytochemistry - Anti-FBXO11 antibody (AB181801)
  • ICC

Supplier Data

Immunocytochemistry - Anti-FBXO11 antibody (AB181801)

Immunocytochemistry analysis of HeLa cells labeling FBXO11 with ab181801 at 5 μg/ml

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Human FBXO11. The exact immunogen used to generate this antibody is proprietary information.

Q86XK2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "5 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXO11 also known as F-box only protein 11 is a member of the F-box protein family which are characterized by an F-box motif a sequence of approximately 40 amino acids. It has an approximate mass of 114 kDa. FBXO11 participates in the ubiquitin-proteasome system by acting as a substrate-recognition component of SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complexes. The protein is expressed in various tissues throughout the body including lung liver and skin.
Biological function summary

FBXO11 is involved in protein degradation and regulation of the cell cycle. It serves as an important component of the SCF ubiquitin ligase complex where it binds to specific substrates marking them for proteasomal degradation. FBXO11 plays an important role in cellular processes such as DNA damage response and cell growth regulation. By targeting specific proteins for degradation it regulates protein stability which is important for normal cellular proliferation.

Pathways

FBXO11 is critical in the TGF-beta signaling pathway and the apoptosis pathway. In the TGF-beta signaling pathway FBXO11 regulates the stability of inhibitory SMAD proteins like SMAD7 by targeting them for proteasomal degradation. This regulation impacts cellular responses to TGF-beta influencing processes like cell differentiation and immune responses. In apoptosis FBXO11 interacts with other proteins like BCL2 to influence cell survival and programmed cell death.

FBXO11 plays a role in specific types of cancer and developmental disorders. Mutations or dysregulation of FBXO11 have identified links to diffuse large B-cell lymphoma due to its ability to affect the degradation of BCL6— a transcriptional repressor involved in the development and growth of this cancer. In developmental disorders FBXO11 mutations have associations with conditions like the oculo-facio-cardio-dental syndrome illustrating its importance in normal developmental processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as DTL/CDT2, BCL6, SNAI1 and PRDM1/BLIMP1 (PubMed : 17098746, PubMed : 22113614, PubMed : 23478441, PubMed : 23478445, PubMed : 23892434, PubMed : 24613396, PubMed : 24968003, PubMed : 25827072, PubMed : 29059170). The SCF(FBXO11) complex mediates ubiquitination and degradation of BCL6, thereby playing a role in the germinal center B-cells terminal differentiation toward memory B-cells and plasma cells (PubMed : 22113614). The SCF(FBXO11) complex also mediates ubiquitination and degradation of DTL, an important step for the regulation of TGF-beta signaling, cell migration and the timing of the cell-cycle progression and exit (PubMed : 23478441, PubMed : 23478445). The SCF(FBXO11) complex also catalyzes ubiquitination and degradation of GSK3B-phosphorylated SNAI1 (PubMed : 25827072, PubMed : 29059170). Binds to and neddylates phosphorylated p53/TP53, inhibiting its transcriptional activity (PubMed : 17098746). Plays a role in the regulatiom of erythropoiesis but not myelopoiesis or megakaryopoiesis (PubMed : 33156908). Mechanistically, activates erythroid genes by mediating the degradation of BAHD1, a heterochromatin-associated protein that recruits corepressors to H3K27me3 marks (PubMed : 33156908). Participates in macrophage cell death and inflammation in response to bacterial toxins by regulating the expression of complement 5a receptor 1/C5AR1 and IL-1beta (PubMed : 33156908). Acts as a critical regulator to determine the level of MHC-II by mediating the recognition of degron at the P/S/T domain of CIITA leading to its ubiquitination and subsequent degradation via the proteasome (PubMed : 37279268). Participates in the antiviral repsonse by initiating the activation of TBK1-IRF3-IFN-I axis (PubMed : 36897010). Mediates the 'Lys-63'-linked ubiquitination of TRAF3 to strengthen the interaction between TRAF3 and TBK1 (PubMed : 36897010).
See full target information FBXO11
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 79:100365 PubMed38677194

2024

Tanshinone IIA alleviates IL-1β-induced chondrocyte apoptosis and inflammation by regulating FBXO11 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Xu,XiaoCheng Zhi,YunHui Zhang,Ren Ding

International journal of chronic obstructive pulmonary disease 16:2105-2118 PubMed34295155

2021

Circ-RBMS1 Knockdown Alleviates CSE-Induced Apoptosis, Inflammation and Oxidative Stress via Up-Regulating FBXO11 Through miR-197-3p in 16HBE Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Di Qiao,Chi Hu,Qiuyan Li,Jun Fan

OncoTargets and therapy 13:9741-9751 PubMed33061453

2020

Long Non-Coding RNA GATA6-AS1 Sponges miR-324-5p to Inhibit Lung Cancer Cell Proliferation and Invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenxing Wang,Liming Pan,Liangliang Yang,Peiyun Lv,Shixiong Mai,Yue Wang

Oncology letters 20:235 PubMed32968457

2020

miR-181a-5p inhibits the proliferation and invasion of drug-resistant glioblastoma cells by targeting F-box protein 11 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyan Wen,Songrong Li,Mengchan Guo,Hongzhan Liao,Yongmin Chen,Xi Kuang,Xiaoping Liao,Lin Ma,Qifu Li

Cell biology international 44:882-893 PubMed31829474

2020

LINC01436, regulating miR-585 and FBXO11, is an oncogenic lncRNA in the progression of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Guangyong Yang,Xiaogu He,Shi Chen,Fan Zhang,Xiangming Fang

Human cell 32:390-396 PubMed31079326

2019

miR-376a inhibits the proliferation and invasion of osteosarcoma by targeting FBXO11.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaolong Xu,Li Cheng,Jianyang Chen,Wenjie Lu,Peinian Wang

American journal of cancer research 8:2116-2129 PubMed30416861

2018

Downregulated miR-621 promotes cell proliferation via targeting CAPRIN1 in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Zhang,Wei You,Haoming Zhou,Zhiqiang Chen,Guoyong Han,Xueliang Zuo,Long Zhang,Jindao Wu,Xuehao Wang

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 101:648-655 PubMed29518611

2018

The tumor suppressive miR-26a regulation of FBXO11 inhibits proliferation, migration and invasion of hepatocellular carcinoma cells.

Applications

WB

Species

Unspecified reactive species

Yue Ma,Fang Deng,Peng Li,Guangyu Chen,Youmao Tao,Hongsheng Wang
View all publications
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