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AB151964

Anti-FBXO16 antibody

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(1 Publication)

Rabbit Polyclonal FBXO16 antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FBXO16 aa 50 to C-terminus.

View Alternative Names

FBX16, FBXO16, F-box only protein 16

1 Images
Western blot - Anti-FBXO16 antibody (AB151964)
  • WB

Unknown

Western blot - Anti-FBXO16 antibody (AB151964)

10% SDS PAGE

All lanes:

Western blot - Anti-FBXO16 antibody (ab151964) at 1/1000 dilution

All lanes:

Mouse heart whole cell lysate at 50 µg

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human FBXO16 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8IX29

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXO16 also known as F-box only protein 16 is a component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. It plays a role in targeting proteins for ubiquitination and subsequent proteasomal degradation. The molecular mass of FBXO16 is approximately 45 kDa. This protein is expressed in various tissues with significant levels observed in the brain and reproductive organs. Its expression pattern suggests an involvement in diverse cellular processes depending on tissue type.
Biological function summary

F-box proteins like FBXO16 are important in regulating the stability of specific proteins. FBXO16 is part of the SCF complex which functions as a molecular machinery to control protein turnover by attaching ubiquitin to target proteins. This process marks them for degradation maintaining cellular protein homeostasis. Proper function of such networks is essential for regulating cell cycle growth and response to environmental stimuli among other cellular processes.

Pathways

FBXO16 plays a role in ubiquitin-proteasome pathways. It interacts within pathways that involve protein degradation impacting cellular processes like cell cycle regulation and apoptosis. Additionally FBXO16 interacts with proteins involved in signaling pathways such as those modulating p53 a critical protein for DNA repair and apoptosis. These interactions highlight FBXO16’s impact on maintaining cellular health and responding to cellular stress.

FBXO16 links to cancers due to its role in protein degradation pathways that control cell proliferation. Alterations in FBXO16 expression or function can disrupt normal regulatory processes leading to tumor development and progression. Furthermore there is evidence suggesting FBXO16 interacts with proteins such as p53 and beta-catenin which are also implicated in cancer pathways. This connection indicates potential FBXO16 involvement in tumorigenesis and highlights its importance as a target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate recognition component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Functions as a putative tumor suppressor by targeting nuclear beta-catenin/CTNNB1 for degradation independently of upstream activating signals, thereby inhibiting epithelial-to-mesenchymal transition (PubMed : 30714168). Controls the ubiquitination and degradation of hnRNPL (PubMed : 34333526). Negatively regulates NF-kappa-B signaling by mediating the polyubiquitination and degradation of RELA (PubMed : 40529355). Inhibits autophagy by promoting 'Lys-48'-linked polyubiquitination of the serine/threonine-protein kinase ULK1 (PubMed : 39384743).
See full target information FBXO16
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 7: PubMed29345618

2018

NECAPs are negative regulators of the AP2 clathrin adaptor complex.

Applications

Unspecified application

Species

Unspecified reactive species

Gwendolyn M Beacham,Edward A Partlow,Jeffrey J Lange,Gunther Hollopeter
View all publications
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Product promise

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