AB133717

Anti-FBXO2 antibody [EPR7328(2)]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal FBXO2 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

FBX2, FBXO2, F-box only protein 2

1 Images

Lab

Western blot - Anti-FBXO2 antibody [EPR7328(2)] (AB133717)

All lanes:

Western blot - Anti-FBXO2 antibody [EPR7328(2)] (ab133717) at 1/10000 dilution

All lanes:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 33 kDa

Observed band size: 35 kDa

false

Carrier free

Anti-FBXO2 antibody [EPR7328(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7328(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXO2 also known as F-box protein 2 is part of the F-box protein family. This protein plays a mechanical role in recognizing specific substrates for ubiquitination a process important for protein degradation. FBXO2 weighs around 32 kDa and mainly expresses in the brain where it regulates protein levels. This localization suggests its significance in neural protein management.

Biological function summary

FBXO2 participates in forming the SCF (SKP1-CUL1-F-box protein) complex a multi-protein E3 ubiquitin ligase complex. It targets glycosylated proteins for degradation which helps control glycoprotein homeostasis in cells. FBXO2 binds to N-linked glycoproteins indicating its specificity towards glycan recognition and processing. This function makes it important for maintaining cellular protein balance.

Pathways

FBXO2 is involved in the ubiquitin-proteasome pathway which is essential for protein turnover and quality control. It also plays a role in the endoplasmic reticulum-associated degradation (ERAD) pathway which manages misfolded proteins. FBXO2 collaborates with proteins such as β-transducin repeat-containing protein (β-TrCP) within these pathways showing its interaction in various cellular mechanisms.

FBXO2 has connections to neurodegenerative disorders and cancer. Aberrations in FBXO2 function are linked to Alzheimer's disease due to its role in protein degradation in neurons. In cancer FBXO2 alteration affects glycoprotein regulation potentially leading to tumor progression and metastasis. The protein relates to other F-box proteins which also influence cellular stability and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Involved in the endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranslocated into the cytosol and promoting their ubiquitination and subsequent degradation. Prevents formation of cytosolic aggregates of unfolded glycoproteins that have been retrotranslocated into the cytosol. Able to recognize and bind denatured glycoproteins, preferentially those of the high-mannose type (By similarity).

See full target information FBXO2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:22574 PubMed39343799

2024

FBXO2 promotes the progression of papillary thyroid carcinoma through the p53 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenke Guo,Yaoqiang Ren,Xinguang Qiu

Journal of experimental & clinical cancer research : CR 42:334 PubMed38057879

2023

CircGPRC5A enhances colorectal cancer progress by stabilizing PPP1CA and inducing YAP dephosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhou Chen,Yidan Li,Kuan He,Jianguo Yang,Qican Deng,Yajun Chen,Zhongxue Fu

Diabetes 69:e1 PubMed31959694

2020

Comment on Liu et al. Aberrant Expression of FBXO2 Disrupts Glucose Homeostasis Through Ubiquitin-Mediated Degradation of Insulin Receptor in Obese Mice. Diabetes 2017;66:689-698.

Applications

Unspecified application

Species

Unspecified reactive species

Siauyen Wong,Rick F Nelson,Peiran Lu,Henry Paulson,Dingbo Lin

Nature neuroscience 22:243-255 PubMed30617258

2019

Pathological priming causes developmental gene network heterochronicity in autistic subject-derived neurons.

Applications

Flow Cyt

Species

Unspecified reactive species

Simon T Schafer,Apua C M Paquola,Shani Stern,David Gosselin,Manching Ku,Monique Pena,Thomas J M Kuret,Marvin Liyanage,Abed AlFatah Mansour,Baptiste N Jaeger,Maria C Marchetto,Christopher K Glass,Jerome Mertens,Fred H Gage

Diabetes 66:689-698 PubMed27932386

2016

Aberrant Expression of FBXO2 Disrupts Glucose Homeostasis Through Ubiquitin-Mediated Degradation of Insulin Receptor in Obese Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Liu,Han Lu,Duanzhuo Li,Xuelian Xiong,Lu Gao,Zhixiang Wu,Yan Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FBXO2 antibody [EPR7328(2)]

Western blot - Anti-FBXO2 antibody [EPR7328(2)] (AB133717)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise