AB179818

Anti-FBXO21 antibody [EPR13163]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal FBXO21 antibody. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

FBX21, KIAA0875, FBXO21, F-box only protein 21

5 Images

Flow Cytometry (Intracellular) - Anti-FBXO21 antibody [EPR13163] (AB179818)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FBXO21 antibody [EPR13163] (AB179818)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling FBXO21 with ab179818 at 1/10 dilution (red),compared toa rabbit IgG negative control (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] (AB179818)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] (AB179818)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling FBXO21 with ab179818 at 1/50 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] (AB179818)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling FBXO21 with ab179818 at 1/50 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-FBXO21 antibody [EPR13163] (AB179818)

All lanes:

Western blot - Anti-FBXO21 antibody [EPR13163] (ab179818) at 1/10000 dilution

Lane 1:

MCF-7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

U-87 MG cell lysate at 10 µg

Predicted band size: 72 kDa

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Supplier Data

Immunoprecipitation - Anti-FBXO21 antibody [EPR13163] (AB179818)

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling FBXO21 with ab179818.

All lanes:

Immunoprecipitation - Anti-FBXO21 antibody [EPR13163] (ab179818)

Predicted band size: 72 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13163

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXO21 also known as F-box only protein 21 is a component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex. It has a mass of approximately 58 kDa. This protein facilitates the ubiquitination process by recognizing specific protein substrates and marking them for degradation by the proteasome. FBXO21 is expressed in various tissues with notable levels observed in the brain and muscle. Its expression patterns suggest a role in several physiological processes which can be tissue-specific.

Biological function summary

FBXO21 contributes to the regulation of protein turnover within the cell. It is a part of the SCF complex which is important in maintaining protein homeostasis. This protein complex targets a range of substrates influencing cell cycle regulation signal transduction and transcription processes. By influencing these processes FBXO21 can impact cell proliferation and differentiation which are vital for normal cellular function.

Pathways

FBXO21 is engaged in the ubiquitin-proteasome pathway a critical cellular system that controls protein degradation. This pathway is essential for various cellular processes including cell cycle control and the response to oxidative stress. FBXO21's activity is closely related to other F-box proteins such as β-TrCP and SKP2 which participate in similar ubiquitination pathways. The interactions and targets of FBXO21 in these pathways are important for its functional roles.

FBXO21 has connections to certain neurodegenerative conditions and muscle diseases. Alterations in the function or expression of FBXO21 can affect proteins like amyloid precursor protein which has associations with Alzheimer's disease. Furthermore dysregulation in muscle protein turnover due to FBXO21 malfunction could contribute to muscular dystrophies where it may interact with proteins like atrogin-1 and MuRF1. Understanding these relationships helps delineate the role FBXO21 plays in disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

See full target information FBXO21

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 20:e13306 PubMed33450132

2021

JUNB-FBXO21-ERK axis promotes cartilage degeneration in osteoarthritis by inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiming Lin,Jianing Miao,Tao Zhang,Ming He,Ziyuan Wang,Xinyuan Feng,Lunhao Bai

eLife 5: PubMed27063938

2016

Lys29-linkage of ASK1 by Skp1-Cullin 1-Fbxo21 ubiquitin ligase complex is required for antiviral innate response.

Applications

Species

Unspecified reactive species

Zhou Yu,Taoyong Chen,Xuelian Li,Mingjin Yang,Songqing Tang,Xuhui Zhu,Yan Gu,Xiaoping Su,Meng Xia,Weihua Li,Xuemin Zhang,Qingqing Wang,Xuetao Cao,Jianli Wang

Cell discovery 1:15034 PubMed27462432

2015

A genome-wide IR-induced RAD51 foci RNAi screen identifies CDC73 involved in chromatin remodeling for DNA repair.

Applications

Unspecified application

Species

Human

Patrick Herr,Cecilia Lundin,Bastiaan Evers,Daniel Ebner,Christina Bauerschmidt,Guy Kingham,Timea Palmai-Pallag,Oliver Mortusewicz,Oliver Frings,Erik Sonnhammer,Thomas Helleday

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FBXO21 antibody [EPR13163]

Flow Cytometry (Intracellular) - Anti-FBXO21 antibody [EPR13163] (AB179818)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] (AB179818)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] (AB179818)

Western blot - Anti-FBXO21 antibody [EPR13163] (AB179818)

Immunoprecipitation - Anti-FBXO21 antibody [EPR13163] (AB179818)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Primary Antibodies

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