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AB250120

Anti-FBXO21 antibody [EPR13163] - BSA and Azide free

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Rabbit Recombinant Monoclonal FBXO21 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

View Alternative Names

FBX21, KIAA0875, FBXO21, F-box only protein 21

5 Images
Flow Cytometry (Intracellular) - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (AB250120)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (AB250120)

This data was developed using ab179818, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling FBXO21 with ab179818 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (AB250120)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (AB250120)

This data was developed using ab179818, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling FBXO21 with ab179818 at 1/50 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (AB250120)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (AB250120)

This data was developed using ab179818, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling FBXO21 with ab179818 at 1/50 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (AB250120)
  • IP

Supplier Data

Immunoprecipitation - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (AB250120)

This data was developed using ab179818, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling FBXO21 with ab179818.

All lanes:

Immunoprecipitation - Anti-FBXO21 antibody [EPR13163] (<a href='/en-us/products/primary-antibodies/fbxo21-antibody-epr13163-ab179818'>ab179818</a>)

Predicted band size: 72 kDa

false

Western blot - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (AB250120)
  • WB

Unknown

Western blot - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (AB250120)

This data was developed using ab179818, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FBXO21 antibody [EPR13163] (<a href='/en-us/products/primary-antibodies/fbxo21-antibody-epr13163-ab179818'>ab179818</a>) at 1/10000 dilution

Lane 1:

MCF-7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

U-87 MG cell lysate at 10 µg

Predicted band size: 72 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13163

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab250120 is the carrier-free version of ab179818.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXO21 also known as F-box only protein 21 is a component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex. It has a mass of approximately 58 kDa. This protein facilitates the ubiquitination process by recognizing specific protein substrates and marking them for degradation by the proteasome. FBXO21 is expressed in various tissues with notable levels observed in the brain and muscle. Its expression patterns suggest a role in several physiological processes which can be tissue-specific.
Biological function summary

FBXO21 contributes to the regulation of protein turnover within the cell. It is a part of the SCF complex which is important in maintaining protein homeostasis. This protein complex targets a range of substrates influencing cell cycle regulation signal transduction and transcription processes. By influencing these processes FBXO21 can impact cell proliferation and differentiation which are vital for normal cellular function.

Pathways

FBXO21 is engaged in the ubiquitin-proteasome pathway a critical cellular system that controls protein degradation. This pathway is essential for various cellular processes including cell cycle control and the response to oxidative stress. FBXO21's activity is closely related to other F-box proteins such as β-TrCP and SKP2 which participate in similar ubiquitination pathways. The interactions and targets of FBXO21 in these pathways are important for its functional roles.

FBXO21 has connections to certain neurodegenerative conditions and muscle diseases. Alterations in the function or expression of FBXO21 can affect proteins like amyloid precursor protein which has associations with Alzheimer's disease. Furthermore dysregulation in muscle protein turnover due to FBXO21 malfunction could contribute to muscular dystrophies where it may interact with proteins like atrogin-1 and MuRF1. Understanding these relationships helps delineate the role FBXO21 plays in disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
See full target information FBXO21
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com