Anti-FBXO22 antibody

• WB

Supplier Data

Western blot - Anti-FBXO22 antibody (AB230395)

All lanes:

Western blot - Anti-FBXO22 antibody (ab230395) at 1/1000 dilution

Lane 1:

Hela whole cell lysate at 20 µg

Lane 2:

MCF7 whole cell lysate at 20 µg

Lane 3:

HepG2 whole cell lysate at 20 µg

Lane 4:

293 whole cell lysate at 20 µg

Lane 5:

JK whole cell lysate at 20 µg

Lane 6:

K562 whole cell lysate at 20 µg

Lane 7:

A549 whole cell lysate at 20 µg

Lane 8:

A431 whole cell lysate at 20 µg

Lane 8:

U251 whole cell lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/20000 dilution

Observed band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-FBXO22 antibody (AB230395)

All lanes:

Western blot - Anti-FBXO22 antibody (ab230395) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/20000 dilution

Observed band size: 45 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO22 antibody (AB230395)

Immunohistochemical analysis of ab230395 diluted at 1 : 200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labelled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO22 antibody (AB230395)

Immunohistochemical analysis of ab230395 diluted at 1 : 200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labelled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody