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AB154068

Anti-FBXO28 antibody

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(2 Publications)

Rabbit Polyclonal FBXO28 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FBXO28 aa 50-350.

View Alternative Names

CENP-30, KIAA0483, FBXO28, F-box only protein 28

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-FBXO28 antibody (AB154068)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FBXO28 antibody (AB154068)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling FBXO28 with ab154068 at 1/500 dilution.
The lower image has been merged with a DNA probe.

Western blot - Anti-FBXO28 antibody (AB154068)
  • WB

Unknown

Western blot - Anti-FBXO28 antibody (AB154068)

10% SDS PAGE

All lanes:

Western blot - Anti-FBXO28 antibody (ab154068) at 1/1000 dilution

Lane 1:

A549 whole cell lysate at 30 µg

Lane 2:

H1299 whole cell lysate at 30 µg

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human FBXO28 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

Q9NVF7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXO28 also known as F-box only protein 28 is an important component of the SCF (Skp1-Cullin-F-box) ubiquitin ligase complex. The molecular mass of FBXO28 is approximately 44 kDa. This protein is expressed in various tissues with noted expression in the brain and heart. As an F-box protein FBXO28 plays an important role in targeting proteins for ubiquitination and subsequent proteasomal degradation.
Biological function summary

FBXO28 functions in regulating cell cycle progression and gene expression. It interacts with specific substrates labeling them for degradation and playing a part in the cell's quality control system. FBXO28 associates as part of the SCF complex which consists of Skp1 Cullin and Rbx1 proteins and helps mediate protein turnover. This protein is key in maintaining cellular homeostasis by eliminating damaged or unneeded proteins.

Pathways

Scientists have identified FBXO28's involvement in the regulation of cell cycle and transcription pathways. It influences the stability of cell cycle regulators linking its activity with the proteasome pathway. FBXO28 also associates with the RNA polymerase II transcription machinery helping control gene expression. Relationships with proteins like CDK1 and Cyclin D mirror its part in cycle control and interactions within transcription pathways highlight its regulatory roles.

Research connects FBXO28 to oncogenic processes particularly in certain cancers due to its regulatory function in cell proliferation. Misregulation of FBXO28 may promote tumor growth revealing its link to cancer pathogenesis. Studies also suggest a possible association with neurological disorders potentially through its expression in brain tissue and interactions with proteins involved in neural pathways raising interest in its broader implications in neurodegenerative diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.
See full target information FBXO28
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 42:2878-2891 PubMed37596321

2023

FBXO28 suppresses liver cancer invasion and metastasis by promoting PKA-dependent SNAI2 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Xinran Qiao,Jingyu Lin,Jiajia Shen,Yang Chen,Liyun Zheng,Hangjiang Ren,Xiaoli Zhao,Hang Yang,Pengyu Li,Zhen Wang

International journal of molecular sciences 19: PubMed29587369

2018

An SCF E3 Ligase Protects Pancreatic β-Cells from Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kanaka Durga Devi Gorrepati,Wei He,Blaz Lupse,Ting Yuan,Kathrin Maedler,Amin Ardestani
View all publications
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