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AB224603

Anti-FBXO3 antibody

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(1 Publication)

Rabbit Polyclonal FBXO3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FBXO3 aa 250-400.

View Alternative Names

FBX3, FBXO3, F-box only protein 3

3 Images
Immunocytochemistry - Anti-FBXO3 antibody (AB224603)
  • ICC

Supplier Data

Immunocytochemistry - Anti-FBXO3 antibody (AB224603)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FBXO3 (green) using ab224603 at 1/100 dilution in ICC/IF.  Secondary antibody : Alexa Fluor® 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO3 antibody (AB224603)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO3 antibody (AB224603)

Paraffin-embedded human skeletal muscle tissue stained for FBXO3 using ab224603 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-FBXO3 antibody (AB224603)
  • WB

Supplier Data

Western blot - Anti-FBXO3 antibody (AB224603)

All lanes:

Western blot - Anti-FBXO3 antibody (ab224603) at 1/200 dilution

All lanes:

Mouse kidney tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FBXO3 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q9UK99

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein FBXO3 also known as F-box only protein 3 plays a significant role in targeting proteins for ubiquitination and subsequent degradation. Its molecular weight approximates 47 kDa and it functions in various tissues across the human body. FBXO3 forms part of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which is essential for the process of tagging unwanted proteins for destruction in the proteasome.
Biological function summary

FBXO3 regulates protein stability within the cell. As a component of the SCF complex it specifically recognizes substrates that need degradation contributing to protein turnover and homeostasis. This FBXO3-mediated regulation is important for controlling the levels of various cellular proteins impacting processes like cell cycle progression and signal transduction. Its ability to interact with multiple substrates helps maintain cellular functions and responses to environmental stimuli.

Pathways

FBXO3 takes part in ubiquitin-dependent proteolysis and is closely related to the NF-kB signaling pathway. It regulates the degradation of proteins that modulate the NF-kB pathway which is essential for immune response and inflammation. It also associates with proteins like SKP1 and CUL1 within the SCF complex facilitating substrate recognition and ubiquitination and ultimately impacting cellular signaling pathways through protein turnover.

FBXO3 has implications in inflammatory disorders and certain cancers. Dysregulation in FBXO3-mediated protein degradation affects the stability of proteins involved in inflammation potentially leading to pathological states like chronic inflammatory conditions. FBXO3 also connects to the protein BTK in relation to signal transduction in some cancers providing potential targets for therapeutic interventions. Understanding the function and regulation of FBXO3 may offer insights into disease mechanisms and treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex, SCF(FBXO3), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 18809579, PubMed : 26037928). Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome (PubMed : 18809579). In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented (PubMed : 18809579). PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation (PubMed : 18809579). The SCF(FBXO3) also acts as a regulator of inflammation by mediating ubiquitination and degradation of FBXL2 in response to lipopolysaccharide (LPS) (PubMed : 26037928). The SCF(FBXO3) complex specifically recognizes FBXL2 phosphorylated at 'Thr-404' and promotes its ubiquitination (By similarity).
See full target information FBXO3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 20:107 PubMed32989386

2020

Evaluation of immune-biomarker expression in high-grade soft-tissue sarcoma: HLA-DQA1 expression as a prognostic marker.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Yun Bae,Kyung Un Choi,Ahrong Kim,So Jung Lee,Kyungbin Kim,Jee Yeon Kim,In Sook Lee,So Hak Chung,Jeung Il Kim
View all publications
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