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AB68067

Anti-FBXO31 antibody

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Mouse Polyclonal FBXO31 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FBXO31.

View Alternative Names

FBX14, FBX31, PP2386, FBXO31, F-box only protein 31

1 Images
Western blot - Anti-FBXO31 antibody (AB68067)
  • WB

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Western blot - Anti-FBXO31 antibody (AB68067)

All lanes:

Western blot - Anti-FBXO31 antibody (ab68067) at 1/500 dilution

Lane 1:

FBXO31 (aa 1-367) transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugated at 1/2500 dilution

Predicted band size: 61 kDa

Observed band size: 40 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FBXO31.

Q5XUX0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXO31 also known as F-box only protein 31 functions as part of the SCF (SKP1-Cullin-F-box) E3 ubiquitin protein ligase complex. It plays a role in marking proteins for proteasomal degradation. The molecular weight of FBXO31 is approximately 73 kDa. Gene expression analysis shows FBXO31 is present in various tissues with notable levels in the brain liver and kidney.
Biological function summary

FBXO31 regulates cell cycle progression and stress response pathways. It forms part of the SCF complex facilitating ubiquitination by recognizing and binding specific substrates. The protein contributes to the regulation of cell cycle checkpoints and is involved in DNA repair processes. This makes FBXO31 important for maintaining genomic stability during cell division and protecting against cellular stress.

Pathways

FBXO31 interacts with mechanisms like the p53 signaling pathway and the ubiquitin-dependent proteolysis pathway. In the p53 signaling pathway FBXO31 targets specific proteins that modulate p53 activity affecting cell cycle arrest and apoptosis. Additionally FBXO31 links to other proteins such as SKP1 and Cullin which are essential components of the SCF complex and participate in substrate ubiquitination.

FBXO31 has associations with cancer particularly breast and liver cancers. FBXO31 levels can influence tumor suppressor functions linking it to the pathogenesis of these cancers. Researchers have observed connections between FBXO31 and p53 mutations which further implicate its role in tumor development and progression. Understanding these associations highlights the potential of FBXO31 as a target for therapeutic strategies in oncology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor.
See full target information FBXO31
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Product promise

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