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AB309145

Anti-FBXO38 antibody [EPR27363-29]

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Rabbit Recombinant Monoclonal FBXO38 antibody. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

SP329, FBXO38, F-box only protein 38

4 Images
Western blot - Anti-FBXO38 antibody [EPR27363-29] (AB309145)
  • WB

Supplier Data

Western blot - Anti-FBXO38 antibody [EPR27363-29] (AB309145)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed isconsistent with what has been described in the literature (PMID : 30804394). The identity of the higher MW band at approximately 260 kDa is unknown. The identity of the lower MW bands at approximately 30-50 kDa is unknown. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 92 seconds

All lanes:

Western blot - Anti-FBXO38 antibody [EPR27363-29] (ab309145) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeti FBXO38 whole cell lysate 20 at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 150 kDa

false

Exposure time: 92s

Western blot - Anti-FBXO38 antibody [EPR27363-29] (AB309145)
  • WB

Supplier Data

Western blot - Anti-FBXO38 antibody [EPR27363-29] (AB309145)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : skeletal muscle (PMID : 14729953). The expression profile/ molecular weight observed isconsistent with what has been described in the literature (PMID : 30804394). The identity of the higher MW band at approximately 260 kDa is unknown. The identity of the lower MW bands at approximately 30-50 kDa is unknown. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : Lane 1 : 26 seconds, lanes : 2-3 : 92 seconds.

All lanes:

Western blot - Anti-FBXO38 antibody [EPR27363-29] (ab309145) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Human testis tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Observed band size: 150 kDa

false

Western blot - Anti-FBXO38 antibody [EPR27363-29] (AB309145)
  • WB

Supplier Data

Western blot - Anti-FBXO38 antibody [EPR27363-29] (AB309145)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed isconsistent with what has been described in the literature (PMID : 30804394). The identity of the higher MW band at approximately 260 kDa is unknown. The identity of the lower MW bands at approximately 30-50 kDa is unknown. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 37 seconds

All lanes:

Western blot - Anti-FBXO38 antibody [EPR27363-29] (ab309145) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 150 kDa

false

Exposure time: 37s

Western blot - Anti-FBXO38 antibody [EPR27363-29] (AB309145)
  • WB

Supplier Data

Western blot - Anti-FBXO38 antibody [EPR27363-29] (AB309145)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : skeletal muscle (PMID : 14729953). The expression profile/ molecular weight observed isconsistent with what has been described in the literature (PMID : 30804394). The identity of the higher MW band at approximately 260 kDa is unknown. The identity of the lower MW bands at approximately 30-50 kDa is unknown. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 26 seconds

All lanes:

Western blot - Anti-FBXO38 antibody [EPR27363-29] (ab309145) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 150 kDa

false

Exposure time: 26s

  • Carrier free

    Anti-FBXO38 antibody [EPR27363-29] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27363-29

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXO38 also known as FBX5 is a member of the F-box protein family which plays a significant role in protein ubiquitination. This process marks proteins for degradation by the ubiquitin-proteasome system an important step for maintaining cellular protein balance. FBXO38 weighs approximately 60 kDa and is part of the SCF (SKP1-CUL1-F-box) complex. This protein is expressed in various tissues with higher levels observed in muscle tissues and some parts of the central nervous system.
Biological function summary

FBXO38 participates in regulating protein stability and turnover as an integral component of the ubiquitin-proteasome system. As part of the SCF complex FBXO38 works in collaboration with SKP1 CUL1 and other F-box proteins to select target proteins for ubiquitination. Its role in this complex positions it as a critical regulator of cell cycle progression apoptosis and signal transduction pathways. By selecting specific proteins for degradation FBXO38 influences key cellular functions and ensures proper cellular responses to environmental changes.

Pathways

The function of FBXO38 aligns with the regulation of the MAPK/ERK pathway and the NF-kB pathway. These pathways are essential for cell proliferation survival and inflammation responses. In the MAPK/ERK pathway FBXO38 interacts with components to ensure timely degradation of key signaling proteins thereby modulating pathway activity. Furthermore FBXO38’s interaction with proteins like IκBα in the NF-kB pathway helps control the transcriptional activity of NF-kB influencing immunological and stress responses.

Altered FBXO38 function has connections to muscular atrophy and certain cancers. Variations or mutations in FBXO38 can lead to disruptions in muscle cell protein turnover contributing to muscle-wasting disorders. Additionally improper FBXO38 activity can affect proteins such as MDM2 in oncogenic pathways influencing cancer development and progression. These associations make FBXO38 a potential therapeutic target for addressing protein dysregulation-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of PDCD1/PD-1, thereby regulating T-cells-mediated immunity (PubMed : 30487606). Required for anti-tumor activity of T-cells by promoting the degradation of PDCD1/PD-1; the PDCD1-mediated inhibitory pathway being exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed : 30487606). May indirectly stimulate the activity of transcription factor KLF7, a regulator of neuronal differentiation, without promoting KLF7 ubiquitination (By similarity).
See full target information FBXO38
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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