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AB153803

Anti-FBXO4/FBX4 antibody

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(2 Publications)

Rabbit Polyclonal FBXO4/FBX4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FBXO4 aa 1-350.

View Alternative Names

FBX4, FBXO4, F-box only protein 4

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-FBXO4/FBX4 antibody (AB153803)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FBXO4/FBX4 antibody (AB153803)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling FBXO4/FBX4 with ab153803 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

Western blot - Anti-FBXO4/FBX4 antibody (AB153803)
  • WB

Unknown

Western blot - Anti-FBXO4/FBX4 antibody (AB153803)

10% SDS PAGE

All lanes:

Western blot - Anti-FBXO4/FBX4 antibody (ab153803) at 1/5000 dilution

All lanes:

HL60 whole cell lysate at 30 µg

Predicted band size: 44 kDa,50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FBXO4 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

Q9UKT5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXO4 also called F-box-only protein 4 or FBX4 acts as an E3 ubiquitin ligase component within the SCF complex. It facilitates the ubiquitination and proteasomal degradation of certain proteins by recognizing phosphorylated substrates. FBXO4's molecular mass is around 45 kDa. This protein expresses across various tissues including the brain and heart and also plays a role in the cell cycle regulation and signal transduction.
Biological function summary

FBXO4 is involved in controlling the stability of important regulatory proteins. It assembles with SKP1 Cul1 and RBX1 to form the SCF complex. One of its known substrates is cyclin D1 which is important in regulating cell cycle progression. Degrading cyclin D1 helps prevent the abnormal cell growth associated with cancer development.

Pathways

FBXO4 functionally integrates into the ubiquitin-proteasome system and cell cycle pathways. In the ubiquitin-proteasome pathway it works alongside other enzymes to ensure targeted protein degradation important for maintaining cellular homeostasis. Inside the cell cycle pathway FBXO4's interaction with cyclin D1 influences transition phases coordinating with other proteins like CDK4 to regulate cell division and growth.

FBXO4 has associations with certain cancers like melanoma and breast cancer. Dysregulation of FBXO4 leading to impaired degradation of cyclin D1 contributes to tumorigenesis and cancer progression. Interaction with proteins such as SKP2 involved in cell cycle regulation and cancer highlights the pathogenic potential of altered FBXO4 function when it impacts cell growth control.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 10531035, PubMed : 18598945, PubMed : 20181953, PubMed : 29142209). Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation (PubMed : 18598945). Recognizes TERF1 and promotes its ubiquitination together with UBE2D1 (PubMed : 16275645, PubMed : 20159592). Promotes ubiquitination of FXR1 following phosphorylation of FXR1 by GSK3B, leading to FXR1 degradation by the proteasome (PubMed : 29142209).
See full target information FBXO4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 47: PubMed33649815

2021

circNRIP1 facilitates keloid progression via FXR1‑mediated upregulation of miR‑503‑3p and miR‑503‑5p.

Applications

Unspecified application

Species

Unspecified reactive species

Baolin Wang,Hang Yin,Hongmei Zhang,Tiantian Wang

Nature genetics 46:588-94 PubMed24793136

2014

Pan-cancer genetic analysis identifies PARK2 as a master regulator of G1/S cyclins.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxing Gong,Travis Ian Zack,Luc G T Morris,Kan Lin,Ellen Hukkelhoven,Radhika Raheja,I-Li Tan,Sevin Turcan,Selvaraju Veeriah,Shasha Meng,Agnes Viale,Steven E Schumacher,Perry Palmedo,Rameen Beroukhim,Timothy A Chan
View all publications
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