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AB194362

Anti-FBXO40 antibody [EPR15005-34]

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(2 Publications)

Rabbit Recombinant Monoclonal FBXO40 antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 2 publications.

View Alternative Names

FBX40, KIAA1195, FBXO40, F-box only protein 40, Muscle disease-related protein

3 Images
Western blot - Anti-FBXO40 antibody [EPR15005-34] (AB194362)
  • WB

Supplier Data

Western blot - Anti-FBXO40 antibody [EPR15005-34] (AB194362)

All lanes:

Western blot - Anti-FBXO40 antibody [EPR15005-34] (ab194362) at 1/10000 dilution

All lanes:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 80 kDa

false

Western blot - Anti-FBXO40 antibody [EPR15005-34] (AB194362)
  • WB

Supplier Data

Western blot - Anti-FBXO40 antibody [EPR15005-34] (AB194362)

All lanes:

Western blot - Anti-FBXO40 antibody [EPR15005-34] (ab194362) at 1/1000 dilution

All lanes:

Rat heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 80 kDa

false

Western blot - Anti-FBXO40 antibody [EPR15005-34] (AB194362)
  • WB

Supplier Data

Western blot - Anti-FBXO40 antibody [EPR15005-34] (AB194362)

All lanes:

Western blot - Anti-FBXO40 antibody [EPR15005-34] (ab194362) at 1/1000 dilution

All lanes:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 80 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15005-34

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXO40 also known as F-box only protein 40 is a member of the F-box protein family which is characterized by containing an approximately 40 amino acid motif known as the F-box. It has a mass of around 48 kDa. Mechanically FBXO40 functions as part of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex targeting specific proteins for ubiquitination and subsequent proteasomal degradation. This protein embeds itself within skeletal muscle tissue where its expression plays an essential role.
Biological function summary

FBXO40 regulates muscle protein turnover and influences muscle mass maintenance. As part of a ubiquitin ligase complex FBXO40 participates actively in marking proteins for degradation which is essential for cellular homeostasis. The regulation of signaling molecules involved in muscle physiology makes FBXO40 an important player in maintaining muscle integrity and function. The activity of FBXO40 can indirectly affect muscle growth and the adaptation of muscle tissue to various physiological conditions.

Pathways

FBXO40 engages in the ubiquitin-proteasome system which is critical for protein quality control and cellular regulation. It works alongside other proteins like SKP1 in the SCF complex which targets specific proteins for degradation ensuring proper protein balance within the cell. The interaction with pathways involving skeletal muscle differentiation and remodeling highlights its role in the precise regulation of cellular signaling mechanisms important for muscle development and function.

Research indicates that FBXO40 may associate with muscular dystrophies characterised by the progressive weakening of muscle mass. It is particularly implicated in the pathology of conditions that affect muscle protein breakdown and synthesis. FBXO40 has also been connected with proteins such as myostatin which inhibit muscle growth suggesting a potential link between disrupted FBXO40 function and muscle-related disorders. The understanding of FBXO40's role could contribute to the development of therapeutic strategies addressing genetic muscle diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex that may function in myogenesis.
See full target information FBXO40
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:111005 PubMed39474068

2024

AMPD3 promotes doxorubicin-induced cardiomyopathy through HSP90α-mediated ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Liting Cheng,Mingxiang Zhu,Xiang Xu,Xin Li,Yongming Yao,Chunlei Liu,Kunlun He

Food & function 15:3669-3679 PubMed38487922

2024

Restorative effects of (+)-epicatechin in a rodent model of aging induced muscle atrophy: underlying mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Israel Ramirez-Sanchez,Viridiana Navarrete-Yañez,Lucia Ramirez,Leonor Galera,Enrique Mendez-Bolaina,Veronica Najera,Guillermo Ceballos,Francisco Villarreal
View all publications
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Product promise

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