AB190688

Anti-FBXO40 antibody [EPR15005]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal FBXO40 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

FBX40, KIAA1195, FBXO40, F-box only protein 40, Muscle disease-related protein

3 Images

Flow Cytometry (Intracellular) - Anti-FBXO40 antibody [EPR15005] (AB190688)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FBXO40 antibody [EPR15005] (AB190688)

Intracellular flow cytometric analysis of2% paraformaldehyde fixed HeLa cells labeling FBXO40 with ab190688 at 1/90 followed byGoat anti rabbit IgG (FITC) at 1/150 (red).Rabbit monoclonal IgG was used as isotype control (green).

Supplier Data

Western blot - Anti-FBXO40 antibody [EPR15005] (AB190688)

All lanes:

Western blot - Anti-FBXO40 antibody [EPR15005] (ab190688) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 20 µg

Lane 2:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 80 kDa

Observed band size: 80 kDa

false

Supplier Data

Western blot - Anti-FBXO40 antibody [EPR15005] (AB190688)

All lanes:

Western blot - Anti-FBXO40 antibody [EPR15005] (ab190688) at 1/10000 dilution

All lanes:

Human fetal heart lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 80 kDa

Observed band size: 80 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15005

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/90", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXO40 also known as F-box only protein 40 is a member of the F-box protein family which is characterized by containing an approximately 40 amino acid motif known as the F-box. It has a mass of around 48 kDa. Mechanically FBXO40 functions as part of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex targeting specific proteins for ubiquitination and subsequent proteasomal degradation. This protein embeds itself within skeletal muscle tissue where its expression plays an essential role.

Biological function summary

FBXO40 regulates muscle protein turnover and influences muscle mass maintenance. As part of a ubiquitin ligase complex FBXO40 participates actively in marking proteins for degradation which is essential for cellular homeostasis. The regulation of signaling molecules involved in muscle physiology makes FBXO40 an important player in maintaining muscle integrity and function. The activity of FBXO40 can indirectly affect muscle growth and the adaptation of muscle tissue to various physiological conditions.

Pathways

FBXO40 engages in the ubiquitin-proteasome system which is critical for protein quality control and cellular regulation. It works alongside other proteins like SKP1 in the SCF complex which targets specific proteins for degradation ensuring proper protein balance within the cell. The interaction with pathways involving skeletal muscle differentiation and remodeling highlights its role in the precise regulation of cellular signaling mechanisms important for muscle development and function.

Research indicates that FBXO40 may associate with muscular dystrophies characterised by the progressive weakening of muscle mass. It is particularly implicated in the pathology of conditions that affect muscle protein breakdown and synthesis. FBXO40 has also been connected with proteins such as myostatin which inhibit muscle growth suggesting a potential link between disrupted FBXO40 function and muscle-related disorders. The understanding of FBXO40's role could contribute to the development of therapeutic strategies addressing genetic muscle diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probable substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex that may function in myogenesis.

See full target information FBXO40

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eado9751 PubMed39919185

2025

Inflammatory responses to acute carbon monoxide poisoning and the role of plasma gelsolin.

Applications

Unspecified application

Species

Unspecified reactive species

Awadhesh K Arya,Kinjal Sethuraman,Jaylyn Waddell,Yong Sung Cha,Yuanyuan Liang,Veena M Bhopale,Abid R Bhat,Zuha Imtiyaz,Alik Dakessian,Yoonsuk Lee,Stephen R Thom

Open medicine (Warsaw, Poland) 19:20240971 PubMed38841177

2024

FBXO45 levels regulated ferroptosis renal tubular epithelial cells in a model of diabetic nephropathy by PLK1.

Applications

Unspecified application

Species

Unspecified reactive species

Bingming Zhu,Yongxuan Hu,Ruishan Wu,Quan Yu,Wangrong Wen

American journal of physiology. Endocrinology and 318:E625-E635 PubMed32101031

2020

Stat3 activation induces insulin resistance via a muscle-specific E3 ubiquitin ligase Fbxo40.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Zhang,Zihong Chen,Ying Wang,David J Tweardy,William E Mitch

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FBXO40 antibody [EPR15005]

Flow Cytometry (Intracellular) - Anti-FBXO40 antibody [EPR15005] (AB190688)

Western blot - Anti-FBXO40 antibody [EPR15005] (AB190688)

Western blot - Anti-FBXO40 antibody [EPR15005] (AB190688)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise