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Rabbit Recombinant Monoclonal FBXO7 antibody. Suitable for IP, WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-FBXO7 antibody [EPR29658-670] (AB324143), expandable thumbnail
  • Immunoprecipitation - Anti-FBXO7 antibody [EPR29658-670] (AB324143), expandable thumbnail
  • Immunoprecipitation - Anti-FBXO7 antibody [EPR29658-670] (AB324143), expandable thumbnail
  • Western blot - Anti-FBXO7 antibody [EPR29658-670] (AB324143), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Not recommended
Mouse
Tested
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Species
Mouse
Dilution info
1/30
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins and plays a role in several biological processes such as cell cycle, cell proliferation, or maintenance of chromosome stability (PubMed:15145941, PubMed:34791250). Recognizes and ubiquitinates BIRC2 and the cell cycle regulator DLGAP5 (PubMed:15145941, PubMed:16510124, PubMed:22212761). Plays a role downstream of PINK1 in the clearance of damaged mitochondria via selective autophagy (mitophagy) by targeting PRKN to dysfunctional depolarized mitochondria. Promotes MFN1 ubiquitination. Mediates the ubiquitination and proteasomal degradation of UXT isoform 2, thereby impairing the NF-kappa-B signaling pathway (PubMed:33010352). Inhibits NF-kappa-B pathway also by promoting the ubiquitination of TRAF2 (PubMed:22212761). Affects the assembly state and activity of the proteasome in the cells including neurons by ubiquitinating the proteasomal subunit PSMA2 via 'Lys-63'-linked polyubiquitin chains (By similarity). Promotes 'Lys-48'-linked polyubiquitination SIRT7, leading to the hydrogen peroxide-induced cell death (PubMed:36646384).

Alternative names

FBX7, FBXO7, F-box only protein 7

Rabbit Recombinant Monoclonal FBXO7 antibody. Suitable for IP, WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29658-670
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-FBXO7 antibody [EPR29658-670] (ab324143), expandable thumbnail

    Western blot - Anti-FBXO7 antibody [EPR29658-670] (ab324143)

    FBXO7 Western blot staining using rabbit Anti-FBXO7 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 21347293; PMID: 23656991), the multiple bands are isoforms.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-FBXO7 antibody [EPR29658-670] (ab324143) at 1/1000 dilution

    Lane 1: HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HEK-293 transfected with siRNA specifically targeting FBXO7 whole cell lysate at 20 µg

    Lane 3: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 65-72 kDa, 36 kDa

    Exposure time: 125s

  • Immunoprecipitation - Anti-FBXO7 antibody [EPR29658-670] (ab324143), expandable thumbnail

    Immunoprecipitation - Anti-FBXO7 antibody [EPR29658-670] (ab324143)

    FBXO7 was immunoprecipitated from 0.35 mg B16-F10 (mouse skin melanoma cell) whole cell lysate with ab324143 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324143 at 1/1000 dilution.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-FBXO7 antibody [EPR29658-670] (ab324143) at 1/1000 dilution

    Lane 1: B16-F10 (mouse skin melanoma cell) whole cell lysate at 10 µg

    Lane 2: ab324143 at 1/30 IP in B16-F10 (mouse skin melanoma cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab324143 in B16-F10 whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 65-72 kDa

    Exposure time: 24s

  • Immunoprecipitation - Anti-FBXO7 antibody [EPR29658-670] (ab324143), expandable thumbnail

    Immunoprecipitation - Anti-FBXO7 antibody [EPR29658-670] (ab324143)

    FBXO7 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab324143 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324143 at 1/1000 dilution.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-FBXO7 antibody [EPR29658-670] (ab324143) at 1/1000 dilution

    Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

    Lane 2: ab324143 at 1/30 IP in HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab324143 in HEK-293 whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 65-72 kDa

    Exposure time: 24s

  • Western blot - Anti-FBXO7 antibody [EPR29658-670] (ab324143), expandable thumbnail

    Western blot - Anti-FBXO7 antibody [EPR29658-670] (ab324143)

    FBXO7 Western blot staining using rabbit Anti-FBXO7 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: liver (PMID: 37753846).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1: 180 seconds; Lanes 2-3: 81 seconds; Lanes 4-6: 180 seconds.

    All lanes: Western blot - Anti-FBXO7 antibody [EPR29658-670] (ab324143) at 1/1000 dilution

    Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Mouse testis tissue lysate at 20 µg

    Lane 3: Mouse liver tissue lysate at 20 µg

    Lane 4: Mouse cerebellum tissue lysate at 20 µg

    Lane 5: B16-F10 (mouse skin melanoma cell) whole cell lysate at 20 µg

    Lane 6: MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 65-72 kDa, 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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