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AB115521

Anti-FBXO9 antibody

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(1 Publication)

Rabbit Polyclonal FBXO9 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FBXO9 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

FBX9, VCIA1, FBXO9, F-box only protein 9, Cross-immune reaction antigen 1, Renal carcinoma antigen NY-REN-57

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO9 antibody (AB115521)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO9 antibody (AB115521)

ab115521 at 5ug/ml staining FBXO9 in Human pancreas tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human FBXO9 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9UK97

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXO9 also known as F-box only protein 9 functions as part of the SCF (SKP1-cullin-F-box) ubiquitin ligase complex. This complex tags proteins for degradation by attaching ubiquitin molecules. FBXO9 specifically acts as a substrate recognition component targeting proteins for ubiquitination and subsequent proteasomal degradation. The mass of FBXO9 is around 47 kDa. It is expressed in various tissues with a significant presence in the lung and spleen.
Biological function summary

FBXO9 participates in the regulation of protein stability and turnover. As a component of the SCF complex it plays a role in controlling cell cycle progression and signal transduction. Its function in recognizing and binding specific proteins identifies them for ubiquitin-mediated degradation regulating protein levels within cells. This process is important for maintaining cellular homeostasis and responding to changes in the cellular environment.

Pathways

FBXO9 interacts with processes involved in protein degradation and cell cycle regulation. It is a part of the ubiquitin-proteasome pathway important for removing damaged or misfolded proteins. In addition FBXO9 influences the cell cycle pathway by targeting regulatory proteins for degradation affecting their availability and function. It works alongside proteins such as SKP1 and cullin-1 in these pathways ensuring precise control over cell cycle progression.

FBXO9 has links to certain types of cancers and neurodegenerative diseases. Aberrations in its function may lead to disruptions in protein homeostasis contributing to tumorigenesis through unchecked proliferation due to altered cell cycle regulation. In neurodegenerative diseases FBXO9's role in protein degradation becomes significant as accumulation of malfunctioning proteins can lead to cell death. Interactions with proteins like p27 a cyclin-dependent kinase inhibitor highlight its importance in these disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins and plays a role in several biological processes such as cell cycle, cell proliferation, or maintenance of chromosome stability (PubMed : 23263282, PubMed : 34480022). Ubiquitinates mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is the activated due to the relief of feedback inhibition by mTORC1 (PubMed : 23263282). Plays a role in maintaining epithelial cell survival by regulating the turn-over of chromatin modulator PRMT4 through ubiquitination and degradation by the proteasomal pathway (PubMed : 34480022). Regulates also PPARgamma stability by facilitating PPARgamma/PPARG ubiquitination and thereby plays a role in adipocyte differentiation (By similarity).
See full target information FBXO9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 29:1520-1536 PubMed32337552

2020

A rare genomic duplication in 2p14 underlies autosomal dominant hearing loss DFNA58.

Applications

Unspecified application

Species

Unspecified reactive species

Karina Lezirovitz,Gleiciele A Vieira-Silva,Ana C Batissoco,Débora Levy,Joao P Kitajima,Alix Trouillet,Ellen Ouyang,Navid Zebarjadi,Juliana Sampaio-Silva,Vinicius Pedroso-Campos,Larissa R Nascimento,Cindy Y Sonoda,Vinícius M Borges,Laura G Vasconcelos,Roberto M O Beck,Signe S Grasel,Daniel J Jagger,Nicolas Grillet,Ricardo F Bento,Regina C Mingroni-Netto,Jeanne Oiticica
View all publications
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