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AB317368

Anti-Fbxw7 antibody

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Rabbit Polyclonal Fbxw7 antibody. Suitable for ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human FBXW7 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

FBW7, FBX30, SEL10, FBXW7, F-box/WD repeat-containing protein 7, Archipelago homolog, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, SEL-10, hCdc4, hAgo, Fbw7, Fbwd6, Fbxw6, F-box/WD repeat-containing protein 7, F-box and WD-40 domain-containing protein 7, F-box protein FBW7, F-box protein Fbxw6, F-box-WD40 repeat protein 6, SEL-10, F-box/WD repeat-containing protein 7

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Fbxw7 Antibody (AB317368)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fbxw7 Antibody (AB317368)

Immunocytochemistry/ Immunofluorescence analysis of 4% Paraformaldehyde-fixed, Triton X-100 permeabilized HepG2 cells staining Fbxw7 using ab317368 at a 1 : 100 dilution, for 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes. DAPI (blue) was used to stain the cell nuclei. Blocking buffer (normal goat serum) at 37°C for 20 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbxw7 Antibody (AB317368)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbxw7 Antibody (AB317368)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat brain tissue labeled with ab317368 at a 1 : 200 dilution followed by conjugation to the secondary antibody and DAB staining.

Flow Cytometry (Intracellular) - Anti-Fbxw7 Antibody (AB317368)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Fbxw7 Antibody (AB317368)

Mouse spleen cells were fixed with 4% PFA for 10min at room temperature, permeabilized with ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ab317368 at 1 : 50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human FBXW7 conjugated to Keyhole Limpet Haemocyanin.

Q969H0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 20% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 17434132, PubMed : 22748924, PubMed : 26976582, PubMed : 28727686, PubMed : 34741373, PubMed : 35395208). Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter brings them to the SCF complex for ubiquitination (PubMed : 17434132, PubMed : 22748924, PubMed : 26774286, PubMed : 26976582, PubMed : 28727686, PubMed : 34741373). Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NFE2L1, NOTCH2, MCL1, MLST8, RICTOR, and probably PSEN1 (PubMed : 11565034, PubMed : 11585921, PubMed : 12354302, PubMed : 14739463, PubMed : 15103331, PubMed : 17558397, PubMed : 17873522, PubMed : 22608923, PubMed : 22748924, PubMed : 25775507, PubMed : 25897075, PubMed : 26976582, PubMed : 28007894, PubMed : 28727686, PubMed : 29149593, PubMed : 34102342). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed : 14739463). Involved in bone homeostasis and negative regulation of osteoclast differentiation (PubMed : 29149593). Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1; CDK1-dependent phosphorylation of NR1D1 is necessary for SCF(FBXW7)-mediated ubiquitination (PubMed : 27238018). Also able to promote 'Lys-63'-linked ubiquitination in response to DNA damage (PubMed : 26774286). The SCF(FBXW7) complex facilitates double-strand break repair following phosphorylation by ATM : phosphorylation promotes localization to sites of double-strand breaks and 'Lys-63'-linked ubiquitination of phosphorylated XRCC4, enhancing DNA non-homologous end joining (PubMed : 26774286).
See full target information FBXW7

Additional targets

Fbxw7,Fbxw7
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Product promise

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