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AB227677

Anti-Fbxw7 antibody [SP237]

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(2 Publications)

Rabbit Recombinant Monoclonal Fbxw7 antibody. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

FBW7, FBX30, SEL10, FBXW7, F-box/WD repeat-containing protein 7, Archipelago homolog, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, SEL-10, hCdc4, hAgo

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbxw7 antibody [SP237] (AB227677)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbxw7 antibody [SP237] (AB227677)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrial adenocarcinoma tissue sections labeling Fbxw7 with ab227677 at 1/100 dilution (1.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Flow Cytometry (Intracellular) - Anti-Fbxw7 antibody [SP237] (AB227677)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Fbxw7 antibody [SP237] (AB227677)

Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Fbxw7 with ab227677 at 1/400 (green) compared with a rabbit isotype control (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbxw7 antibody [SP237] (AB227677)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbxw7 antibody [SP237] (AB227677)

Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Fbxw7 with ab227677 at 1/100 dilution in immunohistochemical analysis.

  • Carrier free

    Anti-Fbxw7 antibody [SP237] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP237

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/400", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.</p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 17434132, PubMed : 22748924, PubMed : 26976582, PubMed : 28727686, PubMed : 34741373, PubMed : 35395208). Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter brings them to the SCF complex for ubiquitination (PubMed : 17434132, PubMed : 22748924, PubMed : 26774286, PubMed : 26976582, PubMed : 28727686, PubMed : 34741373). Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NFE2L1, NOTCH2, MCL1, MLST8, RICTOR, and probably PSEN1 (PubMed : 11565034, PubMed : 11585921, PubMed : 12354302, PubMed : 14739463, PubMed : 15103331, PubMed : 17558397, PubMed : 17873522, PubMed : 22608923, PubMed : 22748924, PubMed : 25775507, PubMed : 25897075, PubMed : 26976582, PubMed : 28007894, PubMed : 28727686, PubMed : 29149593, PubMed : 34102342). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed : 14739463). Involved in bone homeostasis and negative regulation of osteoclast differentiation (PubMed : 29149593). Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1; CDK1-dependent phosphorylation of NR1D1 is necessary for SCF(FBXW7)-mediated ubiquitination (PubMed : 27238018). Also able to promote 'Lys-63'-linked ubiquitination in response to DNA damage (PubMed : 26774286). The SCF(FBXW7) complex facilitates double-strand break repair following phosphorylation by ATM : phosphorylation promotes localization to sites of double-strand breaks and 'Lys-63'-linked ubiquitination of phosphorylated XRCC4, enhancing DNA non-homologous end joining (PubMed : 26774286).
See full target information FBXW7
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biology 40: PubMed32094308

2020

E2F6-Mediated Downregulation of MIR22HG Facilitates the Progression of Laryngocarcinoma by Targeting the miR-5000-3p/FBXW7 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Chen,Mudunov Ali,Azizyan Ruben,Dimitry Stelmakh,Maksim Pak

Bioscience reports 40: PubMed32159214

2020

Astragalus polysaccharides inhibit ovarian cancer cell growth via microRNA-27a/FBXW7 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanling Guo,Zhenxing Zhang,Zhaoxia Wang,Guoqi Liu,Yingying Liu,Huijie Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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