Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (ab316199)

Rabbit Recombinant Monoclonal Fc epsilon RI/FCER1A antibody. Suitable for mIHC, WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (AB316199), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	WB	IHC-P	ICC/IF	Flow Cyt	IP
Human	Tested	Tested	Tested	Not recommended	Not recommended	Not recommended
Mouse	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Target data

See full target information FCER1A

Function

High-affinity receptor for immunoglobulin epsilon/IgE. Mediates IgE effector functions in myeloid cells. Upon IgE binding and antigen/allergen cross-linking initiates signaling pathways that lead to myeloid cell activation and differentiation. On mast cells, basophils and eosinophils stimulates the secretion of vasoactive amines, lipid mediators and cytokines that contribute to inflammatory response, tissue remodeling and cytotoxicity against microbes. Triggers the immediate hypersensitivity response to allergens as a host defense mechanism against helminth parasites, pathogenic bacteria and venom toxicity. When dysregulated, it can elicit harmful life-threatening allergic and anaphylactic reactions.

Alternative names

FCE1A, FCER1A, High affinity immunoglobulin epsilon receptor subunit alpha, Fc-epsilon RI-alpha, IgE Fc receptor subunit alpha, FcERI

Recommended products

Rabbit Recombinant Monoclonal Fc epsilon RI/FCER1A antibody. Suitable for mIHC, WB, IHC-P and reacts with Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28402-78
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (ab316199)
Fc epsilon RI/FCER1A Western blot staining using rabbit Anti-Fc epsilon RI/FCER1A antibody
Negative control: HEK-293 (PMID: 17728245).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (ab316199) at 1/1000 dilution
Lane 1: KU812 (human Peripheral blood basophil) whole cell lysate at 40 µg with 5% NFDM/TBST
Lane 2: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 40 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 45 kDa, 124 kDa
Exposure time: 180s
Multiplex immunohistochemistry - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (ab316199)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human lung tissue staining Fc epsilon RI/FCER1A with ab316199 at a 1:500 (1.04 ug/ml) dilution and SIGLEC6 with Anti-SIGLEC6 antibody [EPR28608-317] ab317307 at 1:100 (4.89 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Fc epsilon RI/FCER1A (green; Opal^™520) and anti-SIGLEC6 (magenta; Opal^™690) on human lung.
Panel B: anti-Fc epsilon RI/FCER1A staining immune cells in human lung.
Panel C: anti-SIGLEC6 staining mast cells and basophils in human lung.
Panel D: Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in two rounds of staining: in the order of ab316199 and Anti-SIGLEC6 antibody [EPR28608-317] ab317307 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Multiplex immunohistochemistry - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (ab316199)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human stomach cancer tissue staining Fc epsilon RI/FCER1A with ab316199 at a 1:500 (1.04 ug/ml) dilution and SIGLEC6 with Anti-SIGLEC6 antibody [EPR28608-317] ab317307 at 1:100 (4.89 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Fc epsilon RI/FCER1A (green; Opal^™520) and anti-SIGLEC6 (magenta; Opal^™690) on human stomach cancer.
Panel B: anti-Fc epsilon RI/FCER1A staining immune cells in human stomach cancer.
Panel C: anti-SIGLEC6 staining mast cells and basophils in human stomach cancer.
Panel D: Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in two rounds of staining: in the order of ab316199 and Anti-SIGLEC6 antibody [EPR28608-317] ab317307 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (ab316199)
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Fc epsilon RI/FCER1A with ab316199 at 1/500 (1.04 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on immune cells of human lung (PMID: 21958156).

The section was incubated with ab316199 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (ab316199)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Fc epsilon RI/FCER1A with ab316199 at 1/500 (1.04 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human tonsil.

The section was incubated with ab316199 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (ab316199)
Immunohistochemical analysis of paraffin-embedded Human squamous cell lung carcinoma tissue labeling Fc epsilon RI/FCER1A with ab316199 at 1/500 (1.04 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on immune cells of human lung carcinoma.

The section was incubated with ab316199 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (ab316199)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Fc epsilon RI/FCER1A with ab316199 at 1/500 (1.04 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human cerebrum.

The section was incubated with ab316199 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (ab316199)
Immunohistochemical analysis of paraffin-embedded (A) KU812(human peripheral blood basophils) cell pellet . (B) HEK-293T (human embryonic kidney epithelial cell) cell pellet . tissue labeling Fc epsilon RI/FCER1A with ab316199 at 1/500 (1.04 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) KU812 cell pellet . No staining on (B) HEK-293T cell pellet.

The section was incubated with ab316199 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Western blot - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (ab316199)
Negative control: brain (PMID: 2964640).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 2964640).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (ab316199) at 1/1000 dilution
Lane 1: Human tonsil tissue lysate at 40 µg with 5% NFDM/TBST
Lane 2: Human brain tissue lysate at 40 µg with 5% NFDM/TBST
Lane 3: Human testis tissue lysate at 40 µg with 5% NFDM/TBST
Lane 4: Human bone marrow tissue lysate at 40 µg with 5% NFDM/TBST
Lane 5: Human skin tissue lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 55-60 kDa, 36 kDa
Exposure time: 180s
Western blot - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (ab316199)
Fc epsilon RI/FCER1A is a glycoprotein of approximately 45 kDa and detected as a 30-kDa band after treated with Peptide:N-glycosidase F (PNGase F).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 2964640).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (ab316199) at 1/1000 dilution
Lane 1: Untreated KU812 (human Peripheral blood basophil) whole cell lysate at 40 µg with 5% NFDM/TBST
Lane 2: KU812 treated with Peptide:N-glycosidase F (PNGase F) whole cell lysate at 40 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 30 kDa, 45 kDa, 36 kDa
Exposure time: 81s