Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] - BSA and Azide free

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] - BSA and Azide free (AB316200)

This data was developed using ab316199, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human stomach cancer tissue staining Fc epsilon RI/FCER1A with ab316199 at a 1 : 500 (1.04 ug/ml) dilution and SIGLEC6 with ab317307 at 1 : 100 (4.89 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-Fc epsilon RI/FCER1A (green; Opal^™520) and anti-SIGLEC6 (magenta; Opal^™690) on human stomach cancer. Panel B : anti-Fc epsilon RI/FCER1A staining immune cells in human stomach cancer.Panel C : anti-SIGLEC6 staining mast cells and basophils in human stomach cancer. Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in two rounds of staining : in the order of ab316199 and ab317307 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] - BSA and Azide free (AB316200)

This data was developed using ab316199, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) KU812(human peripheral blood basophils) cell pellet . (B) HEK-293T (human embryonic kidney epithelial cell) cell pellet . tissue labeling Fc epsilon RI/FCER1A with ab316199 at 1/500 (1.04 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) KU812 cell pellet . No staining on (B) HEK-293T cell pellet.
The section was incubated with ab316199 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] - BSA and Azide free (AB316200)

This data was developed using ab316199, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human squamous cell lung carcinoma tissue labeling Fc epsilon RI/FCER1A with ab316199 at 1/500 (1.04 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on immune cells of human lung carcinoma.
The section was incubated with ab316199 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] - BSA and Azide free (AB316200)

This data was developed using ab316199, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human lung tissue staining Fc epsilon RI/FCER1A with ab316199 at a 1 : 500 (1.04 ug/ml) dilution and SIGLEC6 with ab317307 at 1 : 100 (4.89 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-Fc epsilon RI/FCER1A (green; Opal^™520) and anti-SIGLEC6 (magenta; Opal^™690) on human lung. Panel B : anti-Fc epsilon RI/FCER1A staining immune cells in human lung.Panel C : anti-SIGLEC6 staining mast cells and basophils in human lung. Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in two rounds of staining : in the order of ab316199 and ab317307 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] - BSA and Azide free (AB316200)

This data was developed using ab316199, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Fc epsilon RI/FCER1A with ab316199 at 1/500 (1.04 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human cerebrum.
The section was incubated with ab316199 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] - BSA and Azide free (AB316200)

This data was developed using ab316199, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Fc epsilon RI/FCER1A with ab316199 at 1/500 (1.04 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human tonsil.
The section was incubated with ab316199 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] - BSA and Azide free (AB316200)

This data was developed using ab316199, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Fc epsilon RI/FCER1A with ab316199 at 1/500 (1.04 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on immune cells of human lung (PMID : 21958156).
The section was incubated with ab316199 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] - BSA and Azide free (AB316200)

This data was developed using ab316199, the same antibody clone in a different buffer formulation.

Fc epsilon RI/FCER1A is a glycoprotein of approximately 45 kDa and detected as a 30-kDa band after treated with Peptide : N-glycosidase F (PNGase F).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 2964640).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (<a href='/en-us/products/primary-antibodies/fc-epsilon-ri-fcer1a-antibody-epr28402-78-ab316199'>ab316199</a>) at 1/1000 dilution

Lane 1:

Untreated KU812 (human Peripheral blood basophil) whole cell lysate at 40 µg

Lane 2:

KU812 treated with Peptide:N-glycosidase F (PNGase F) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa,45 kDa,36 kDa

true

Exposure time: 81s

• WB

Supplier Data

Western blot - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] - BSA and Azide free (AB316200)

This data was developed using ab316199, the same antibody clone in a different buffer formulation.

Negative control : HEK-293 (PMID : 17728245).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (<a href='/en-us/products/primary-antibodies/fc-epsilon-ri-fcer1a-antibody-epr28402-78-ab316199'>ab316199</a>) at 1/1000 dilution

Lane 1:

KU812 (human Peripheral blood basophil) whole cell lysate at 40 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] - BSA and Azide free (AB316200)

This data was developed using ab316199, the same antibody clone in a different buffer formulation.

Negative control : brain (PMID : 2964640).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 2964640).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (<a href='/en-us/products/primary-antibodies/fc-epsilon-ri-fcer1a-antibody-epr28402-78-ab316199'>ab316199</a>) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 40 µg

Lane 2:

Human brain tissue lysate at 40 µg

Lane 3:

Human testis tissue lysate at 40 µg

Lane 4:

Human bone marrow tissue lysate at 40 µg

Lane 5:

Human skin tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55-60 kDa,36 kDa

false

Exposure time: 180s