AB223381

Anti-FCGRT/FCRN antibody [CL3640]

Name: Anti-FCGRT/FCRN antibody [CL3640] (ab223381) | Abcam
Brand: Abcam Limited
SKU: AB223381

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(1 Publication)

Mouse Monoclonal FCGRT/FCRN antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FCGRT aa 150-300.

View Alternative Names

FCRN, FCGRT, IgG receptor FcRn large subunit p51, FcRn, IgG Fc fragment receptor transporter alpha chain, Neonatal Fc receptor

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [CL3640] (AB223381)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [CL3640] (AB223381)

Paraffin embedded human lymph node tissue stained for FCGRT/FCRN with ab223381 at a 1/5000 dilution in Immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [CL3640] (AB223381)

Paraffin embedded human colon tissue stained for FCGRT/FCRN with ab223381 at a 1/5000 dilution in Immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [CL3640] (AB223381)

Paraffin embedded human liver tissue stained for FCGRT/FCRN with ab223381 at a 1/5000 dilution in Immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [CL3640] (AB223381)

Paraffin embedded human placenta tissue stained for FCGRT/FCRN with ab223381 at a 1/5000 dilution in Immunohistochemical analysis.

Supplier Data

Western blot - Anti-FCGRT/FCRN antibody [CL3640] (AB223381)

All lanes:

Western blot - Anti-FCGRT/FCRN antibody [CL3640] (ab223381) at 1/500 dilution

All lanes:

THP-1 (human monocytic leukemia cell line) whole cell lysate

Predicted band size: 40 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3640

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human FCGRT aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

P55899

Epitope

Binds to an epitope located within the peptide sequence GQGDFGPNSD as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein FCGRT also known as FCRN or FcRn has a significant role in the immune system by binding to the Fc region of immunoglobulin G (IgG). This binding prolongs the half-life of IgG by preventing its lysosomal degradation. The molecular mass of the FCGRT protein is roughly 52 kDa. It is prominently expressed in epithelial and endothelial cells across several tissues including the placenta and liver ensuring IgG stability in circulation.

Biological function summary

FCGRT/FCRN is involved in the transportation and recycling of IgG antibodies. It forms a complex with beta-2 microglobulin that contributes to its function. This complex allows IgG antibodies to traverse cellular barriers such as epithelial cells without being digested. This protein is important in maintaining humoral immunity by regulating IgG levels in the bloodstream and similar processes can be observed in mouse FCRN and cynomolgus monkey milk indicating a conserved mechanism across species.

Pathways

FCGRT/FCRN plays a part in the neonatal Fc receptor pathway. This pathway influences IgG homeostasis and impacts antigen presentation to T cells. By doing so it affects immune surveillance and immune tolerance mechanisms. The pathway involves interactions with related proteins such as albumin which also binds to FCRN for extending its half-life in serum.

Disruptions in FCGRT/FCRN functionality relate to autoimmune conditions and inflammatory diseases. Conditions such as lupus and myasthenia gravis show altered IgG recycling or transportation implicating FCRN in their pathology. Accordingly researchers have explored FCRN inhibitors as potential therapeutic interventions. These inhibitors aim to modulate the interaction between FCRN and IgG therefore impacting the disease progression linked to these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface receptor that transfers passive humoral immunity from the mother to the newborn. Binds to the Fc region of monomeric immunoglobulin gamma and mediates its selective uptake from milk (PubMed : 10933786, PubMed : 7964511). IgG in the milk is bound at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids. Throughout life, contributes to effective humoral immunity by recycling IgG and extending its half-life in the circulation. Mechanistically, monomeric IgG binding to FcRn in acidic endosomes of endothelial and hematopoietic cells recycles IgG to the cell surface where it is released into the circulation (PubMed : 10998088). In addition of IgG, regulates homeostasis of the other most abundant circulating protein albumin/ALB (PubMed : 24469444, PubMed : 28330995).. (Microbial infection) Acts as an uncoating receptor for a panel of echoviruses including Echovirus 5, 6, 7, 9, 11, 13, 25 and 29.

See full target information FCGRT

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 46:101996 PubMed38795560

2024

Regulatory feedback loop between circ-EIF4A3 and EIF4A3 Enhances autophagy and growth in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qingke Li,Zhiwu Wang,Jian Wang,Jiangong Wang,Xuan Zheng,Dan Li,Zhuo Wang,Jingwu Li,Yufeng Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FCGRT/FCRN antibody [CL3640]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [CL3640] (AB223381)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [CL3640] (AB223381)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [CL3640] (AB223381)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [CL3640] (AB223381)

Western blot - Anti-FCGRT/FCRN antibody [CL3640] (AB223381)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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