AB256121

Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal FCGRT/FCRN antibody. Carrier free. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

FCRN, FCGRT, IgG receptor FcRn large subunit p51, FcRn, IgG Fc fragment receptor transporter alpha chain, Neonatal Fc receptor

6 Images

Flow Cytometry - Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free (AB256121)

Flow Cyt

Unknown

Flow Cytometry - Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free (AB256121)

Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labeling FCGRT/FCRN with ab228975 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Bright fluorophore (like AF647) conjugated secondary antibodies are recommended to get good separation.

Negative control : HeLa (PMID : 21216798).

Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228975).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free (AB256121)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free (AB256121)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling FCGRT/FCRN with ab228975 at 1/4000 dilution (0.14 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on endothelial cells of human spleen (PMID : 28402755) is observed. The section was incubated with ab228975 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228975).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free (AB256121)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling FCGRT/FCRN with ab228975 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen, arrows indicate the macrophages (PMID : 28402755) is observed .The section was incubated with ab228975 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228975).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free (AB256121)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling FCGRT/FCRN with ab228975 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human liver (PMID : 28402755) is observed. The section was incubated with ab228975 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228975).

Unknown

Immunoprecipitation - Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free (AB256121)

FCGRT/FCRN was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab228975 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab228975 1/1000 dilution (0.55 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

Lane 1 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10μg
Lane 2 : ab228975 IP in HEK-293 whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab228975 in HEK-293 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 100 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228975).

All lanes:

Immunoprecipitation - Anti-FCGRT/FCRN antibody [EPR22627-25] (<a href='/en-us/products/primary-antibodies/fcgrt-fcrn-antibody-epr22627-25-ab228975'>ab228975</a>)

Predicted band size: 40 kDa

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Lab

Western blot - Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free (AB256121)

This data was developed using the same antibody clone in a different buffer formulation (ab228975).

Lanes 1-4 : Merged signal (red and green). Green - ab228975 observed at 40 kDa. Red - loading control ab8245 observed at 38 kDa.

ab228975 Anti-FCGRT/FCRN antibody [EPR22627-25] was shown to specifically react with FCGRT/FCRN in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab265075 (knockout cell lysate ab257435) was used. Wild-type and FCGRT/FCRN knockout samples were subjected to SDS-PAGE. ab228975 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FCGRT/FCRN antibody [EPR22627-25] (<a href='/en-us/products/primary-antibodies/fcgrt-fcrn-antibody-epr22627-25-ab228975'>ab228975</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (non-ATCC) cell lysate at 20 µg

Lane 2:

Western blot - Human FCGRT (FCRN) knockout HEK-293T cell lysate (<a href='/en-us/products/cell-lysates/human-fcgrt-fcrn-knockout-hek-293t-cell-lysate-ab257435'>ab257435</a>)

Lane 2:

Human FCGRT (FCRN) knockout HEK-293T (non-ATCC) cell lysate (<a href='/en-us/products/cell-lysates/human-fcgrt-fcrn-knockout-hek-293t-cell-lysate-ab257435'>ab257435</a>)

Lane 3:

HEK293 (ATCC) cell lysate at 20 µg

Lane 4:

LNCaP cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22627-25

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab256121 is the carrier-free version of ab228975.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein FCGRT also known as FCRN or FcRn has a significant role in the immune system by binding to the Fc region of immunoglobulin G (IgG). This binding prolongs the half-life of IgG by preventing its lysosomal degradation. The molecular mass of the FCGRT protein is roughly 52 kDa. It is prominently expressed in epithelial and endothelial cells across several tissues including the placenta and liver ensuring IgG stability in circulation.

Biological function summary

FCGRT/FCRN is involved in the transportation and recycling of IgG antibodies. It forms a complex with beta-2 microglobulin that contributes to its function. This complex allows IgG antibodies to traverse cellular barriers such as epithelial cells without being digested. This protein is important in maintaining humoral immunity by regulating IgG levels in the bloodstream and similar processes can be observed in mouse FCRN and cynomolgus monkey milk indicating a conserved mechanism across species.

Pathways

FCGRT/FCRN plays a part in the neonatal Fc receptor pathway. This pathway influences IgG homeostasis and impacts antigen presentation to T cells. By doing so it affects immune surveillance and immune tolerance mechanisms. The pathway involves interactions with related proteins such as albumin which also binds to FCRN for extending its half-life in serum.

Disruptions in FCGRT/FCRN functionality relate to autoimmune conditions and inflammatory diseases. Conditions such as lupus and myasthenia gravis show altered IgG recycling or transportation implicating FCRN in their pathology. Accordingly researchers have explored FCRN inhibitors as potential therapeutic interventions. These inhibitors aim to modulate the interaction between FCRN and IgG therefore impacting the disease progression linked to these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface receptor that transfers passive humoral immunity from the mother to the newborn. Binds to the Fc region of monomeric immunoglobulin gamma and mediates its selective uptake from milk (PubMed : 10933786, PubMed : 7964511). IgG in the milk is bound at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids. Throughout life, contributes to effective humoral immunity by recycling IgG and extending its half-life in the circulation. Mechanistically, monomeric IgG binding to FcRn in acidic endosomes of endothelial and hematopoietic cells recycles IgG to the cell surface where it is released into the circulation (PubMed : 10998088). In addition of IgG, regulates homeostasis of the other most abundant circulating protein albumin/ALB (PubMed : 24469444, PubMed : 28330995).. (Microbial infection) Acts as an uncoating receptor for a panel of echoviruses including Echovirus 5, 6, 7, 9, 11, 13, 25 and 29.

See full target information FCGRT

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:681 PubMed41053117

2025

ALIX mediates reversible gasdermin-D pore formation via the endosomal pathway to limit pyroptosis by active membrane repair.

Applications

Unspecified application

Species

Unspecified reactive species

Sylvia Otchere,Prafulla Shrestha,Himesh N Parmar,Jadyn F Perry,Brittany L Hofmeister,Michelle Steyn,Radhey S Kaushik,Adam D Hoppe,Natalie W Thiex,Ryan L Hanson,Jaime Lopez-Mosqueda,Gergely Imre

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free

Flow Cytometry - Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free (AB256121)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free (AB256121)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free (AB256121)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free (AB256121)

Immunoprecipitation - Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free (AB256121)

Western blot - Anti-FCGRT/FCRN antibody [EPR22627-25] - BSA and Azide free (AB256121)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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