JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB272640

Anti-FCHO1 antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal FCHO1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FCHO1 aa 250-400.

View Alternative Names

KIAA0290, FCHO1, F-BAR domain only protein 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-FCHO1 antibody (AB272640)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FCHO1 antibody (AB272640)

PFA fixed, Triton X-100 permeablized human cell line MCF7 labeling FCHO1 (green) using ab272640 at 2 ug/ml in ICC/IF analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCHO1 antibody (AB272640)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCHO1 antibody (AB272640)

Paraffin-embedded formalin-fixed Human appendix tissue stained for FCHO1 using ab272640 at 1/500 in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FCHO1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

O14526

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FCHO1 also known as FCH domain only 1 is a protein involved in clathrin-mediated endocytosis playing an important role in the formation of clathrin-coated pits on the plasma membrane. FCHO1 has a molecular mass of approximately 89 kDa. It is expressed in various tissues with higher expression reported in the brain and heart. FCHO1 contains F-BAR domains that facilitate membrane curvature which helps in the recruitment and assembly of clathrin and other scaffold proteins at the cell membrane.
Biological function summary

FCHO1 interacts with other proteins to drive the initial events in the endocytic process. It forms part of a protein complex that includes AP2 and Eps15 which are essential for the sculpting and pinching off of vesicles. This complex modulates the efficiency and specificity of endocytosis a process integral to cellular uptake of nutrients receptors and maintenance of cellular homeostasis. FCHO1 initiates the recruitment of clathrin to the membrane which is an important step in forming vesicles that contain loaded cargo for transport into the cell.

Pathways

FCHO1 is a pivotal player in the clathrin-mediated endocytosis pathway. This pathway is critical for efficient internalization of specific receptors like receptors for nutrients and hormones and involves interaction with proteins such as clathrin and dynamin. These proteins contribute to vesicle formation and pinching off therefore driving the pathway forward. Moreover FCHO1 also interacts with the AP2 adaptor complex which helps in recognizing cargo proteins and assembling them into budding vesicles.

FCHO1 mutations have been linked to neurodevelopmental disorders specifically intellectual disability. Its dysfunction can affect neuronal signaling pathways due to impaired endocytosis leading to altered synaptic transmission. Research also suggests a potential connection between FCHO1 and cancer as improper regulation of endocytosis may contribute to oncogenic pathways. FCHO1's interaction with proteins like clathrin and AP2 further elucidates its role in such disease mechanisms making it a target of interest for therapeutic exploration.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Functions in an early step of clathrin-mediated endocytosis (PubMed : 30822429). Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. May regulate Bmp signaling by regulating clathrin-mediated endocytosis of Bmp receptors. Involved in the regulation of T-cell poliferation and activation (PubMed : 30822429, PubMed : 32098969). Affects TCR clustering upon receptor triggering and modulates its internalisation, playing a role in TCR-dependent T-cell activation (PubMed : 32098969).
See full target information FCHO1
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB272666

Anti-PINCH-2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PINCH-2 antibody (AB272666)

  • 0
  • 0
Primary Antibodies

AB272649

Anti-NHE-7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHE-7 antibody (AB272649)

  • 0
  • 0
Primary Antibodies

AB272657

Anti-VKORC1L1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-VKORC1L1 antibody (AB272657)

  • 0
  • 0
Primary Antibodies

AB272668

Anti-HSD17B6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B6 antibody (AB272668)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com