Rabbit Polyclonal FCHO2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human F-BAR domain only protein 2 aa 250-350.
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Publications
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- The Journal of cell biology 221:2022Induced nanoscale membrane curvature bypasses the essential endocytic function of clathrin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert C Cail,Cyna R Shirazinejad,David G DrubinPubMed 35532382
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human F-BAR domain only protein 2 aa 250-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q0JRZ9
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target data
Function
Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a lipid-binding activity with a preference for membranes enriched in phosphatidylserine and phosphoinositides (Pi(4,5) biphosphate) like the plasma membrane. Its membrane-bending activity might be important for the subsequent action of clathrin and adaptors in the formation of clathrin-coated vesicles. Involved in adaptor protein complex AP-2-dependent endocytosis of the transferrin receptor, it also functions in the AP-2-independent endocytosis of the LDL receptor.
Alternative names
F-BAR domain only protein 2, FCHO2
Recommended products
Rabbit Polyclonal FCHO2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human F-BAR domain only protein 2 aa 250-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human F-BAR domain only protein 2 aa 250-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q0JRZ9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FCHO2 protein also known as F-BAR domain-containing protein 2 plays an important role in the process of endocytosis. It has a molecular mass of approximately 82 kDa. FCHO2 is involved in the initial stages of clathrin-mediated endocytosis and recruits other proteins to the plasma membrane to initiate vesicle formation. This protein is primarily expressed in the brain and immune system tissues where efficient vesicular transport is important for proper cell communication and response.
- Biological function summary
FCHO2 forms part of a protein complex that includes epsin and AP-2 which are necessary for membrane curvature and cargo selection during endocytosis. By influencing membrane invagination FCHO2 impacts membrane dynamics and intracellular trafficking. The ability of FCHO2 to bind to phospholipids is important for the accurate targeting of these endocytic proteins to membrane sites impacting various cellular processes such as signal transduction and nutrient uptake.
- Pathways
The role of FCHO2 is critical in the endocytic pathway which is important for the internalization of surface receptors and ligands. FCHO2 interacts with clathrin and accessory proteins to coordinate vesicle scission from the plasma membrane. It has functional relationships with other proteins such as clathrin heavy chain which ensures accuracy in vesicle formation and the sorting of cellular cargo.
- Associated diseases and disorders
Alterations in FCHO2 function have implications in neurological disorders and immune deficiencies. Dysregulation of endocytosis where FCHO2 is essential can lead to problems in neuronal signaling and immune responses. FCHO2 interacts with proteins like dynamin which are implicated in conditions such as Charcot-Marie-Tooth disease. Moreover incorrect FCHO2 activity may contribute to defects in synaptic vesicle recycling impacting neurodegenerative disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCHO2 antibody (ab122407)
ab122407, at a 1/40 dilution, staining FCHO2 in glandular cells of paraffin-embedded Human endometrium tissue by immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCHO2 antibody (ab122407)
ab122407, at a 1/40 dilution, staining FCHO2 in glandular cells of paraffin-embedded Human placenta tissue by immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCHO2 antibody (ab122407)
ab122407, at a 1/40 dilution, staining FCHO2 in thyroid gland cells of paraffin-embedded Human small intestine tissue by immunohistochemistry.
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