JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB122485

Anti-FCHSD2 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal FCHSD2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FCHSD2.

View Alternative Names

KIAA0769, SH3MD3, FCHSD2, F-BAR and double SH3 domains protein 2, Carom, Protein nervous wreck 1, SH3 multiple domains protein 3, NWK1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCHSD2 antibody (AB122485)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCHSD2 antibody (AB122485)

ab122485, at 1/50, staining FCHSD2 in paraffin embedded Human pancreas tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-FCHSD2 antibody (AB122485)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FCHSD2 antibody (AB122485)

Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus, plasma membrane and cytoskeleton (actin filaments). Recommended concentration of ab122485 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Western blot - Anti-FCHSD2 antibody (AB122485)
  • WB

Unknown

Western blot - Anti-FCHSD2 antibody (AB122485)

All lanes:

Western blot - Anti-FCHSD2 antibody (ab122485) at 1/250 dilution

Lane 1:

RT 4 cell line lysate

Lane 2:

U 251 MG cell line lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 84 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human FCHSD2. The exact immunogen used to generate this antibody is proprietary information.

O94868

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FCHSD2 also known as F-BAR and double SH3 domain-containing protein 2 is a protein that mechanically regulates membrane dynamics and cytoskeletal arrangements. It has a molecular mass of approximately 74 kDa and is expressed in various tissues with high expression levels in the brain and heart. FCHSD2 is an adapter protein that is involved in shaping membrane curvature and plays a role in the endocytosis process by interacting with membranes.
Biological function summary

FCHSD2 connects cytoskeletal networks with membrane structures influencing cellular shape and movement. It is part of a multiprotein complex that modulates actin filament organization. FCHSD2 interacts with other proteins that participate in similar structural functions facilitating the coordination of cellular architecture. This protein contributes to maintaining cellular integrity by responding to signals that require cytoskeletal rearrangement.

Pathways

FCHSD2 participates in the endocytosis pathway a critical process for molecular transport across cell membranes. It interacts with proteins like dynamin which is essential for membrane scission events. The protein also plays a role in actin cytoskeleton reorganization alongside others such as cortactin contributing to cellular mobility and changes in cell shape. FCHSD2 helps ensure these processes function properly by linking them to membrane dynamics.

Aberrations in FCHSD2 function associate with neurological conditions and cardiac disorders. In the brain altered FCHSD2 expression may contribute to neurodegenerative diseases because of its role in regulating neuronal membrane dynamics and cytoskeletal interactions. In cardiology FCHSD2 has connections to proteins such as filamin and disruptions in these interactions can affect heart function possibly leading to cardiomyopathies. Understanding FCHSD2's involvement could be key in developing therapeutic approaches for these diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Adapter protein that plays a role in endocytosis via clathrin-coated pits. Contributes to the internalization of cell surface receptors, such as integrin ITGB1 and transferrin receptor (PubMed : 29887380). Promotes endocytosis of EGFR in cancer cells, and thereby contributes to the down-regulation of EGFR signaling (PubMed : 30249660). Recruited to clathrin-coated pits during a mid-to-late stage of assembly, where it is required for normal progress from U-shaped intermediate stage pits to terminal, omega-shaped pits (PubMed : 29887380). Binds to membranes enriched in phosphatidylinositol 3,4-bisphosphate or phosphatidylinositol 3,4,5-trisphosphate (PubMed : 29887380). When bound to membranes, promotes actin polymerization via its interaction with WAS and/or WASL which leads to the activation of the Arp2/3 complex. Does not promote actin polymerisation in the absence of membranes (PubMed : 29887380).
See full target information FCHSD2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinica chimica acta; international journal of clinical chemistry 478:18-27 PubMed29253494

2017

Angiopoietin-2 (Ang-2) is a useful serum tumor marker for liver cancer in the Chinese population.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxin Chen,Yanping Wu,Xiao Zhang,Hong Zeng,Ya Liu,Qi Wu,Yan Chen,GuoQing Zhu,Qiuhui Pan,Lei Jin,Lin Guo,Fenyong Sun
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
Primary Antibodies

AB125011

Anti-TSG101 antibody [EPR7130(B)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-TSG101 antibody [EPR7130(B)] (AB125011)

  • 4
  • 10
Primary Antibodies

AB76494

Anti-SYN2 antibody [EPR3277]

RabMAb|Recombinant|Trial Size

Western blot - Anti-SYN2 antibody [EPR3277] (AB76494)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com