AB151986

Anti-FcRgamma antibody

Name: Anti-FcRgamma antibody (ab151986) | Abcam
Brand: Abcam Limited
SKU: AB151986
Price: 510 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal FcRgamma antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Recombinant fragment samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human FCER1G aa 1 to C-terminus.

View Alternative Names

High affinity immunoglobulin epsilon receptor subunit gamma, Fc receptor gamma-chain, Fc-epsilon RI-gamma, IgE Fc receptor subunit gamma, FcRgamma, FceRI gamma, FCER1G

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody (AB151986)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody (AB151986)

Immunohistochemical analysis of paraffin-embedded U873 xenograft tissue labeling FcRgamma with ab151986 at 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-FcRgamma antibody (AB151986)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FcRgamma antibody (AB151986)

Immunofluorescence analysis of HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min labelling FCER1G protein at cell membrane using ab151986 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] at a 1/1000 dilution (Red). DAPI was used for nuclear staining (Blue).
Scale bar= 10 µm.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FcRgamma antibody (AB151986)

Immunofluorescence analysis of THP-1 cells fixed in 4% paraformaldehyde at RT for 15 min labelling FCER1G protein at cytoplasm using ab151986 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).

Supplier Data

Western blot - Anti-FcRgamma antibody (AB151986)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-FcRgamma antibody (ab151986) at 1/5000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

DDDDK tagged FCER1G transfected 293T whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 10 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human FCER1G aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P30273

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)." }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein containing an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors. As a component of the high-affinity immunoglobulin E (IgE) receptor, mediates allergic inflammatory signaling in mast cells. As a constitutive component of interleukin-3 receptor complex, selectively mediates interleukin 4/IL4 production by basophils, priming T-cells toward effector T-helper 2 subset. Associates with pattern recognition receptors CLEC4D and CLEC4E to form a functional signaling complex in myeloid cells. Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of ITAM, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes. May function cooperatively with other activating receptors. Functionally linked to integrin beta-2/ITGB2-mediated neutrophil activation. Also involved in integrin alpha-2/ITGA2-mediated platelet activation.

See full target information FCER1G

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed39755578

2025

Spatial and single-cell transcriptomics reveal cellular heterogeneity and a novel cancer-promoting Treg cell subset in human clear-cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiyu Song,Yumeng Zhu,Wenwen Geng,Jianhua Jiao,Hongjiao Liu,Ruo Chen,Qian He,Lijuan Wang,Xiuxuan Sun,Weijun Qin,Jiejie Geng,Zhinan Chen

Cell reports 42:113413 PubMed38096059

2023

Synonymous codon usage regulates translation initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Chloe L Barrington,Gabriel Galindo,Amanda L Koch,Emma R Horton,Evan J Morrison,Samantha Tisa,Timothy J Stasevich,Olivia S Rissland

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2205397 PubMed36727833

2023

Neuronal C-Reactive Protein/FcγRI Positive Feedback Proinflammatory Signaling Contributes to Nerve Injury Induced Neuropathic Pain.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Liu,Li Zhang,Si Su,Yehong Fang,Xiang-Sha Yin,Huan Cui,Jianru Sun,Yikuan Xie,Chao Ma

BMC cancer 22:140 PubMed35120484

2022

FCER1G positively relates to macrophage infiltration in clear cell renal cell carcinoma and contributes to unfavorable prognosis by regulating tumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Keqin Dong,Wenjin Chen,Xiuwu Pan,Hongru Wang,Ye Sun,Cheng Qian,Weijie Chen,Chao Wang,Fu Yang,Xingang Cui

Cancer cell international 21:103 PubMed33579299

2021

Prognostic and predictive value of FCER1G in glioma outcomes and response to immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Houshi Xu,Qingwei Zhu,Lan Tang,Junkun Jiang,Huiwen Yuan,Anke Zhang,Meiqing Lou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FcRgamma antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody (AB151986)

Immunocytochemistry/ Immunofluorescence - Anti-FcRgamma antibody (AB151986)

Immunocytochemistry/ Immunofluorescence - Anti-FcRgamma antibody (AB151986)

Western blot - Anti-FcRgamma antibody (AB151986)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise