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Rabbit Recombinant Monoclonal FcRgamma antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Transfected cell lysate, Human, Cat, Dog samples. Immunogen corresponding to Synthetic Peptide within Human FCER1G conjugated to Keyhole Limpet Haemocyanin.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849), expandable thumbnail
  • Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human FCER1G conjugated to Keyhole Limpet Haemocyanin. Database link P30273

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Expected
Tested
Expected
Mouse
Tested
Expected
Tested
Cat
Expected
Expected
Tested
Dog
Expected
Expected
Tested
Transfected cell lysate
Tested
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000.00000 - 1/10000.00000
Notes

-

Species
Transfected cell lysate
Dilution info
1/1000.00000 - 1/10000.00000
Notes

-

Expected
Expected

Species
Human, Cat, Dog
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Cat, Dog
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Cat, Dog
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate
Dilution info
-
Notes

-

Target data

Function

Adapter protein containing an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors. As a component of the high-affinity immunoglobulin E (IgE) receptor, mediates allergic inflammatory signaling in mast cells (PubMed:14764707). As a constitutive component of interleukin-3 receptor complex, selectively mediates interleukin 4/IL4 production by basophils, priming T-cells toward effector T-helper 2 subset (PubMed:19098920). Associates with pattern recognition receptors CLEC4D and CLEC4E to form a functional signaling complex in myeloid cells. Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of ITAM, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes (Probable) (PubMed:23602766). May function cooperatively with other activating receptors. Functionally linked to integrin beta-2/ITGB2-mediated neutrophil activation (PubMed:17086186). Also involved in integrin alpha-2/ITGA2-mediated platelet activation (PubMed:9171347).

Alternative names

Fce1g, Fcer1g, High affinity immunoglobulin epsilon receptor subunit gamma, Fc receptor gamma-chain, Fc-epsilon RI-gamma, IgE Fc receptor subunit gamma, FcRgamma, FceRI gamma

Recommended products

Rabbit Recombinant Monoclonal FcRgamma antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Transfected cell lysate, Human, Cat, Dog samples. Immunogen corresponding to Synthetic Peptide within Human FCER1G conjugated to Keyhole Limpet Haemocyanin.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human FCER1G conjugated to Keyhole Limpet Haemocyanin. Database link P30273
Carrier free
Yes
Clone number
HL1418
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849)

    Immunofluorescence analysis of 4% paraformaldehyde fixed THP-1 cells labelling FcRgamma protein at cell membrane using ab313849 at a 1/500 dilution at RT for 15 min (Green). The nuclear counter stain is DAPI (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849)

    Paraffin-embedded mouse spleen tissue stained for FcRgamma protein at cell membrane with ab313849 at a 1/100 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849)

    Paraffin-embedded cat cerebellum tissue stained for FcRgamma protein at cell membrane with ab313849 at a 1/100 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849)

    Paraffin-embedded dog cerebellum tissue stained for FcRgamma protein at cell membrane with ab313849 at a 1/100 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849), expandable thumbnail

    Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849)

    Samples were seperated by 15% SDS-PAGE.

    All lanes: Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849) at 1/1000 dilution

    All lanes: Raw264.7 whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 9 kDa

  • Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849), expandable thumbnail

    Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849)

    All lanes: Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849) at 1/1000 dilution

    All lanes: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 10 kDa

  • Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849), expandable thumbnail

    Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849)

    All lanes: Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849) at 1/5000 dilution

    Lane 1: Untransfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 30 µg

    Lane 2: DDDDK-tag tagged FcRgamma (FCER1G) transfected HEK293T cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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