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AB313849

Anti-FcRgamma antibody [HL1418] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal FcRgamma antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Transfected cell lysate, Human, Cat, Dog samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FCER1G conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Fce1g, Fcer1g, High affinity immunoglobulin epsilon receptor subunit gamma, Fc receptor gamma-chain, Fc-epsilon RI-gamma, IgE Fc receptor subunit gamma, FcRgamma, FceRI gamma

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849)

Immunofluorescence analysis of 4% paraformaldehyde fixed THP-1 cells labelling FcRgamma protein at cell membrane using ab313849 at a 1/500 dilution at RT for 15 min (Green). The nuclear counter stain is DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849)

Paraffin-embedded mouse spleen tissue stained for FcRgamma protein at cell membrane with ab313849 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849)
  • WB

Supplier Data

Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849) at 1/1000 dilution

All lanes:

Raw264.7 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 9 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849)

Paraffin-embedded dog cerebellum tissue stained for FcRgamma protein at cell membrane with ab313849 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849)

Paraffin-embedded cat cerebellum tissue stained for FcRgamma protein at cell membrane with ab313849 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849)
  • WB

Supplier Data

Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (AB313849)

All lanes:

Western blot - Anti-FcRgamma antibody [HL1418] - BSA and Azide free (ab313849) at 1/5000 dilution

Lane 1:

Untransfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 30 µg

Lane 2:

DDDDK-tag tagged FcRgamma (FCER1G) transfected HEK293T cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1418

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human, Cat, Dog

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human FCER1G conjugated to Keyhole Limpet Haemocyanin.

P30273

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Cat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Dog": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein containing an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors. As a component of the high-affinity immunoglobulin E (IgE) receptor, mediates allergic inflammatory signaling in mast cells (PubMed : 14764707). As a constitutive component of interleukin-3 receptor complex, selectively mediates interleukin 4/IL4 production by basophils, priming T-cells toward effector T-helper 2 subset (PubMed : 19098920). Associates with pattern recognition receptors CLEC4D and CLEC4E to form a functional signaling complex in myeloid cells. Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of ITAM, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes (Probable) (PubMed : 23602766). May function cooperatively with other activating receptors. Functionally linked to integrin beta-2/ITGB2-mediated neutrophil activation (PubMed : 17086186). Also involved in integrin alpha-2/ITGA2-mediated platelet activation (PubMed : 9171347).
See full target information Fcer1g
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:15583 PubMed40320408

2025

Irisin suppressed the progression of TBI via modulating AMPK/MerTK/autophagy and SYK/ROS/inflammatory signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kuo Zhang,Yihui Du,Sihui Yang,Guozhu Sun
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com