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AB308065

Anti-FCRL1+FCRL2 antibody [EPR26947-62] - BSA and Azide free

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Rabbit Recombinant Monoclonal FCRL2 antibody. Carrier free. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Human, Transfected cell line - Human samples.

View Alternative Names

CD307b, FCRH2, IFGP4, IRTA4, SPAP1, UNQ9236/PRO31998, FCRL2, Fc receptor-like protein 2, FcR-like protein 2, FcRL2, Fc receptor homolog 2, IFGP family protein 4, Immunoglobulin receptor translocation-associated protein 4, SH2 domain-containing phosphatase anchor protein 1, FcRH2, CD307a, FCRH1, IFGP1, IRTA5, FCRL1, Fc receptor-like protein 1, FcR-like protein 1, FcRL1, Fc receptor homolog 1, IFGP family protein 1, Immune receptor translocation-associated protein 5, FcRH1, hIFGP1

4 Images
Flow Cytometry (Intracellular) - Anti-FCRL1+FCRL2 antibody [EPR26947-62] - BSA and Azide free (AB308065)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FCRL1+FCRL2 antibody [EPR26947-62] - BSA and Azide free (AB308065)

This data was developed using ab308064, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized HEK-293T cells transfected with Myc-tagged FCRL3 overexpression construct / HEK-293T cells transfected with Myc-tagged FCRL4 overexpression construct / HEK-293T cells transfected with Myc-tagged FCRL5 overexpression construct cells labelling FCRL1+FCRL2 with ab308064 at 1/50 dilution (1ug), compared with a Rabbit monoclonal IgG (ab172730) isotype control (left). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were surface stained with ab308064, then fixed with 2% PFA for 10 min followed by intracellularly staining with anti-Myc tag conjugated to Alexa Fluor® 647.

Flow Cytometry - Anti-FCRL1+FCRL2 antibody [EPR26947-62] - BSA and Azide free (AB308065)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-FCRL1+FCRL2 antibody [EPR26947-62] - BSA and Azide free (AB308065)

This data was developed using ab308064, the same antibody clone in a different buffer formulation. Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) cells labelling FCRL1+FCRL2 with ab308064 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) isotype control / Left. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab308064, then co-stained with anti-CD19 conjugated to Alexa Fluor® 647. Gated on viable cells.

Flow Cytometry (Intracellular) - Anti-FCRL1+FCRL2 antibody [EPR26947-62] - BSA and Azide free (AB308065)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FCRL1+FCRL2 antibody [EPR26947-62] - BSA and Azide free (AB308065)

This data was developed using ab308064, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized HEK-293T cells transfected with Myc-tagged FCRL1 overexpression construct (Middle) and HEK-293T cells transfected with Myc-tagged FCRL2 overexpression construct (Right) cells labelling FCRL1+FCRL2 with ab308064 at 1/50 dilution (1ug), compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were surface stained with ab308064, then fixed with 2% PFA for 10 min followed by intracellularly staining with anti-Myc tag conjugated to Alexa Fluor® 647.

Flow Cytometry (Intracellular) - Anti-FCRL1+FCRL2 antibody [EPR26947-62] - BSA and Azide free (AB308065)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FCRL1+FCRL2 antibody [EPR26947-62] - BSA and Azide free (AB308065)

This data was developed using ab308064, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized HEK-293T cells transfected with Myc-tagged FCRL3 overexpression construct / HEK-293T cells transfected with Myc-tagged FCRL4 overexpression construct / HEK-293T cells transfected with Myc-tagged FCRL5 overexpression construct cells labelling FCRL1+FCRL2 with ab308064 at 1/50 dilution (1ug), compared with a Rabbit monoclonal IgG (ab172730) isotype control (left).

A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with ab308064, then fixed with 2% PFA for 10 min followed by intracellularly staining with anti-Myc tag conjugated to Alexa Fluor® 647.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26947-62

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell line - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FCRL1 and FCRL2 are proteins part of the Fc receptor-like (FCRL) family. FCRL1 also known as Fc receptor-like protein 1 is a membrane-bound protein weighing approximately 98 kDa. It is mainly expressed on mature B lymphocytes. FCRL2 also known as Fc receptor-like protein 2 has an estimated mass of 100 kDa and is also found on B cells but at varying levels depending on the cell's stage and condition. Expression of these receptors occurs principally within the immune system hinting at their regulatory roles in immune responses.
Biological function summary

These proteins are integral to immune function. FCRL1 participates in the regulation of B cell activation and antibody production while FCRL2 modulates B cell responses during immune processes. Both proteins can interact with various immunoglobulins suggesting their role in modulating signaling pathways associated with B cell receptor activity. FCRL1 and FCRL2 appear to be components of larger regulatory complexes on the B cell surface coordinating immune response based on extracellular signals.

Pathways

FCRL1 and FCRL2 are involved in B cell signaling networks. They form important components of pathways like the B cell receptor (BCR) signaling and the immune regulation network. Both proteins are associated with other receptor proteins such as CD19 and CD21 playing a part in fine-tuning the signaling processes that influence B cell maturation and antibody responses. Their activity influences not just BCR signaling but also other related immune regulatory pathways bridging external signals and B cell functionality.

Malfunction or misregulation of FCRL1 and FCRL2 is linked with autoimmune diseases and B cell malignancies like systemic lupus erythematosus and chronic lymphocytic leukemia. These proteins when deregulated may contribute to altered B cell proliferation or inappropriate immune signaling. In these diseases the involvement of proteins like CD22 and CD40 indicates that FCRL1 and FCRL2 have broader roles in immune dysregulation impacting disease pathogenesis and progression. Understanding their function and interactions could lead to targeted therapies and better disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May have an regulatory role in normal and neoplastic B cell development.
See full target information FCRL2

Additional targets

FCRL1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com