AB251250

Anti-FCRL2 antibody [EPR16122] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal FCRL2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD307b, FCRH2, IFGP4, IRTA4, SPAP1, UNQ9236/PRO31998, FCRL2, Fc receptor-like protein 2, FcR-like protein 2, FcRL2, Fc receptor homolog 2, IFGP family protein 4, Immunoglobulin receptor translocation-associated protein 4, SH2 domain-containing phosphatase anchor protein 1, FcRH2

4 Images

Supplier Data

Immunoprecipitation - Anti-FCRL2 antibody [EPR16122] - BSA and Azide free (AB251250)

This data was developed using ab197897, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of 293 (Human epithelial cells from embryonic kidney) whole cell extract labeling FCRL2 using ab197897 at 1/60 dilution (Lane 2).
Lane 3 : IP using Rabbit monoclonal IgG (ab172730) ) instead of ab197897 in 293 (Human epithelial cells from embryonic kidney) whole cell extract.
Lane 1 : Input : 10 μg 293 (Human epithelial cells from embryonic kidney) whole cell extract.
Subsequent WB detection was performed using ab197897 at 1/1000 dilution.
An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-FCRL2 antibody [EPR16122] (<a href='/en-us/products/primary-antibodies/fcrl2-antibody-epr16122-ab197897'>ab197897</a>)

Predicted band size: 56 kDa

false

Supplier Data

Western blot - Anti-FCRL2 antibody [EPR16122] - BSA and Azide free (AB251250)

This data was developed using ab197897, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FCRL2 antibody [EPR16122] (<a href='/en-us/products/primary-antibodies/fcrl2-antibody-epr16122-ab197897'>ab197897</a>) at 1/1000 dilution

All lanes:

NAMALWA (human Burkitt's lymphoma ) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-FCRL2 antibody [EPR16122] - BSA and Azide free (AB251250)

This data was developed using ab197897, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FCRL2 antibody [EPR16122] (<a href='/en-us/products/primary-antibodies/fcrl2-antibody-epr16122-ab197897'>ab197897</a>) at 1/1000 dilution

All lanes:

Human tonsil lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-FCRL2 antibody [EPR16122] - BSA and Azide free (AB251250)

This data was developed using ab197897, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FCRL2 antibody [EPR16122] (<a href='/en-us/products/primary-antibodies/fcrl2-antibody-epr16122-ab197897'>ab197897</a>) at 1/1000 dilution

All lanes:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

false

Exposure time: 30s

Unconjugated

Anti-FCRL2 antibody [EPR16122]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16122

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab251250 is the carrier-free version of ab197897.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FCRL2 also known as FC Receptor-like 2 or CD307b belongs to the family of Fc receptor-like molecules. It is a transmembrane receptor with a mass of approximately 110 kDa. FCRL2 shows expression mainly in B cells with higher levels notably in some mature B cell subtypes found in the lymphoid tissues. Its expression is significantly observed in the follicles of the spleen and tonsils indicating a role in the immune responses centered around these tissues.

Biological function summary

FCRL2 modulates signaling in B cells partaking in immune regulation. It participates in the regulatory networks that adjust B-cell function and differentiation but it does not form any known complex with other proteins. The protein has a structural similarity to classical Fc receptors that suggests a modulatory role in immune response. Researchers consider FCRL2 significant in altering B cell receptor signaling though the exact ligands remain under active investigation.

Pathways

The protein FCRL2 integrates into immune signaling pathways such as the B cell receptor (BCR) signaling pathway. This pathway plays a direct role in B cell activation and antibody production. FCRL2 interacts with other FCRLS members in such pathways to maintain immune homeostasis. The extent of its integration implies a fine-tuning of B cell functions and links to Fcγ receptors indicating a shared regulatory network in pathways.

FCRL2 associates with conditions like chronic lymphocytic leukemia (CLL) and autoimmune diseases. Increased expression of FCRL2 often correlates with certain leukemia subtypes hinting at its potential as a biomarker for disease progression. Moreover its dysregulation may contribute to autoimmune conditions where B cell dysfunctions are central. Studies have also connected FCRL2 with other immune-regulatory proteins in these diseases further highlighting its relevance in pathological conditions related to immune response dysregulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May have an regulatory role in normal and neoplastic B cell development.

See full target information FCRL2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 16: PubMed38732558

2024

Differential Modulation by Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) of Mesenteric Fat and Macrophages and T Cells in Adipose Tissue of Obese / Zucker Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Hong,Peter Zahradka,Carla G Taylor

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FCRL2 antibody [EPR16122] - BSA and Azide free

Immunoprecipitation - Anti-FCRL2 antibody [EPR16122] - BSA and Azide free (AB251250)

Western blot - Anti-FCRL2 antibody [EPR16122] - BSA and Azide free (AB251250)

Western blot - Anti-FCRL2 antibody [EPR16122] - BSA and Azide free (AB251250)

Western blot - Anti-FCRL2 antibody [EPR16122] - BSA and Azide free (AB251250)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise