Anti-FCRL5 antibody [EPR27365-87] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-FCRL5 antibody [EPR27365-87] - BSA and Azide free (AB307731)

This data was developed using ab307730, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : Jurkat (PMID : 24504816). Low expression : human kidney (PMID : 11290337). The bands beneath the target band (120 kDa) are likely to be degraded target fragments. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : Lane 1 and 2 : 103 seconds, Lane 3 and 4 : 26 seconds.

All lanes:

Western blot - Anti-FCRL5 antibody [EPR27365-87] (<a href='/en-us/products/primary-antibodies/fcrl5-antibody-epr27365-87-ab307730'>ab307730</a>) at 1/1000 dilution

Lane 1:

NAMALWA (human burkitts lymphoma b lymphocyte) whole cell lysate at 40 µg

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 40 µg

Lane 3:

Human tonsil tissue lysate at 40 µg

Lane 4:

Human kidney tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 120 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-FCRL5 antibody [EPR27365-87] - BSA and Azide free (AB307731)

This data was developed using ab307730, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human FCRL1, FCRL2, FCRL3, FCRL4 and FCRL6. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : 3 seconds

All lanes:

Western blot - Anti-FCRL5 antibody [EPR27365-87] (<a href='/en-us/products/primary-antibodies/fcrl5-antibody-epr27365-87-ab307730'>ab307730</a>) at 1/1000 dilution

Lane 1:

HEK-293T cells transfected with a human FCRL1 expression vector containi a his tag whole cell lysate at 20 µg

Lane 2:

HEK-293T cells transfected with a human FCRL2 expression vector containi a his tag whole cell lysate at 20 µg

Lane 3:

HEK-293T cells transfected with a human FCRL3 expression vector containi a his tag whole cell lysate at 20 µg

Lane 4:

HEK-293T cells transfected with a human FCRL4 expression vector containi a his tag whole cell lysate at 20 µg

Lane 5:

HEK-293T cells transfected with a human FCRL5 expression vector containi a his tag whole cell lysate at 20 µg

Lane 6:

HEK-293T cells transfected with a human FCRL6 expression vector containi a his tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 120 kDa

false

Exposure time: 3s