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AB121420

Anti-FDCSP antibody

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(1 Publication)

Rabbit Polyclonal FDCSP antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FDCSP aa 1 to C-terminus.

View Alternative Names

C4orf7, UNQ733/PRO1419, FDCSP, Follicular dendritic cell secreted peptide, FDC secreted protein, FDC-SP

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FDCSP antibody (AB121420)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FDCSP antibody (AB121420)

Immunohistochemical analysis of human salivary gland labeling FDCSP with ab121420 at 1/50 dilution. Moderate cytoplasmic positivity in a subset of glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FDCSP antibody (AB121420)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FDCSP antibody (AB121420)

Immunohistochemical analysis of human tonsil and skeletal muscle tissue labeling FDCSP with ab121420 at 1/50 dilution.

Immunohistochemistry - Anti-FDCSP antibody (AB121420)
  • IHC

CiteAb

Immunohistochemistry - Anti-FDCSP antibody (AB121420)

Immunohistochemistry using Anti-FDCSP antibody, ab121420. Publication image from Puri, K. D. et al., 2018, Cell, 30270042. Legend direct from paper.

Colonic Mesenchymal Plasticity in IBD(A) t-SNE plot of UC colonic mesenchyme dataset. Single cells colored by cluster annotation. Descriptive cluster labels are shown.(B) Human healthy and UC cluster marker gene overlap correlation heatmap.(C) Selected enriched (FDR < 0.01) GO terms of UC S4 mesenchymal population marker genes.(D) (i) Flow cytometry analysis of CD74 and PDPN expression on colonic stromal cells from Ctrl (right) or UC (left) donors. (ii) Comparison of intracellular CCL19 and IL-33 levels in CD74highPDPNhighCD24high cells (red) versus the corresponding CD74lowPDPNlow subset (blue) in inflamed UC colonic tissue.(E) Flow cytometry analysis of FDCSPhigh and CD24high colonic stromal cells from Ctrl (blue) or UC (red).(F) Single-molecule ISH staining of FDCSP in Ctrl or UC colonic tissue sections.(G) Flow cytometric analysis of SOX6 expression in Ctrl (blue) or UC (red) colonic stromal cells.See also Figures S1 and S3 and Tables S1 and S5.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FDCSP aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NFU4

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Complementary Products

Primary Antibodies

AB307335

Alexa Fluor® 555 Anti-CD3 antibody [SP162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD3 antibody [SP162] (AB307335)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB139238

Recombinant Human FDCSP protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human FDCSP protein (His tag N-Terminus) (AB139238)

  • 0
  • 0
Primary Antibodies

AB89568

Anti-LTB antibody [MM0462-3J26]

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein FDCSP known as Follicular Dendritic Cell Secreted Protein possesses a molecular mass of approximately 8.6 kDa. Researchers find FDCSP mainly expressed in follicular dendritic cells within lymphoid tissues. This protein is small but it holds significant importance in the immune system's architecture. Due to its precise expression pattern FDCSP serves as a useful marker in studies of lymphoid structures and functions.
Biological function summary

FDCSP plays an important role in the organization of lymphoid follicles and the maintenance of the lymph node microenvironment. It associates with the extracellular matrix supporting communication between dendritic cells and other immune cells. Although not part of a large complex FDCSP influences the network surrounding follicular dendritic cells. This role aids in antigen presentation and lymphocyte activation key elements in the adaptive immune response.

Pathways

FDCSP connects closely with immune response pathways particularly those regulating antigen presentation and lymphocyte activation. It interacts indirectly with proteins involved in the complement system such as C3 and with receptors on B-lymphocytes. This interaction aids in creating a robust immune response by enhancing antigen presentation and promoting B cell maturation within germinal centers.

FDCSP draws attention because of its involvement in immune-related conditions like autoimmune diseases and lymphomas. Its expression levels may change in diseases such as follicular lymphoma impacting the disease progression. Connections to other proteins such as BAFF (B-cell activating factor) highlight FDCSP's role in B-cell related disorders. Understanding FDCSP's behavior in these contexts helps to shed light on its potential as a diagnostic or therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Can bind to the surface of B-lymphoma cells, but not T-lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.
See full target information FDCSP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 175:372-386.e17 PubMed30270042

2018

Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease.

Applications

Unspecified application

Species

Unspecified reactive species

James Kinchen,Hannah H Chen,Kaushal Parikh,Agne Antanaviciute,Marta Jagielowicz,David Fawkner-Corbett,Neil Ashley,Laura Cubitt,Esther Mellado-Gomez,Moustafa Attar,Eshita Sharma,Quin Wills,Rory Bowden,Felix C Richter,David Ahern,Kamal D Puri,Jill Henault,Francois Gervais,Hashem Koohy,Alison Simmons
View all publications
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Product promise

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