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Rabbit Recombinant Monoclonal FDFT1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

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Images

Western blot - Anti-FDFT1 antibody [EPR16481] (AB195046), expandable thumbnail
  • Western blot - Anti-FDFT1 antibody [EPR16481] (AB195046), expandable thumbnail
  • Western blot - Anti-FDFT1 antibody [EPR16481] (AB195046), expandable thumbnail
  • Immunoprecipitation - Anti-FDFT1 antibody [EPR16481] (AB195046), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FDFT1 antibody [EPR16481] (AB195046), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/70
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/16000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway.

Alternative names

Squalene synthase, SQS, SS, FPP:FPP farnesyltransferase, Farnesyl-diphosphate farnesyltransferase, Farnesyl-diphosphate farnesyltransferase 1, FDFT1

Recommended products

Rabbit Recombinant Monoclonal FDFT1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16481
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FDFT1 also known as squalene synthase catalyzes the first committed step in cholesterol biosynthesis. This enzyme functions as a dimer and has a molecular weight of approximately 47 kDa. FDFT1 is widely expressed in the liver where cholesterol synthesis primarily occurs but you can also find it present in many tissues throughout the body. As a membrane-bound protein it anchors to the endoplasmic reticulum where it carries out its enzymatic role.

Biological function summary

FDFT1 facilitates the conversion of farnesyl diphosphate to squalene a critical precursor in sterol and cholesterol synthesis. This enzyme does not work in isolation but acts as a part of the larger sterol biosynthesis pathway. The activity of FDFT1 aligns with key metabolic demands particularly in tissues with high rates of cell division or hormone production making it essential for maintaining cellular sterol homeostasis.

Pathways

FDFT1 is an important enzyme in the mevalonate pathway and cholesterol biosynthesis pathway. This enzyme interacts closely with proteins such as HMG-CoA reductase and lanosterol synthase which are pivotal in these pathways. This relationship ensures the proper flow from acetyl-CoA to cholesterol and other isoprenoids critical for cell membrane integrity and signaling functions.

Associated diseases and disorders

Disruptions in FDFT1 activity can lead to conditions like hypercholesterolemia and related cardiovascular diseases. Excess or deficiency in its function impacts cholesterol levels directly making it a target for cholesterol-lowering therapies. FDFT1's activity also connects to the proteins NPC1 and LDL receptor which play a role in cholesterol transport and uptake further linking it to pathways that contribute to atherosclerosis when dysregulated.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-FDFT1 antibody [EPR16481] (ab195046), expandable thumbnail

    Western blot - Anti-FDFT1 antibody [EPR16481] (ab195046)

    All lanes: Western blot - Anti-FDFT1 antibody [EPR16481] (ab195046) at 1/10000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human fetal spleen lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 48 kDa

  • Western blot - Anti-FDFT1 antibody [EPR16481] (ab195046), expandable thumbnail

    Western blot - Anti-FDFT1 antibody [EPR16481] (ab195046)

    All lanes: Western blot - Anti-FDFT1 antibody [EPR16481] (ab195046) at 1/10000 dilution

    All lanes: HepG2 cell lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 48 kDa

  • Western blot - Anti-FDFT1 antibody [EPR16481] (ab195046), expandable thumbnail

    Western blot - Anti-FDFT1 antibody [EPR16481] (ab195046)

    All lanes: Western blot - Anti-FDFT1 antibody [EPR16481] (ab195046) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Rat kidney lysate at 10 µg

    Lane 3: Rat spleen lysate at 10 µg

    Lane 4: RAW264.7 lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa

  • Immunoprecipitation - Anti-FDFT1 antibody [EPR16481] (ab195046), expandable thumbnail

    Immunoprecipitation - Anti-FDFT1 antibody [EPR16481] (ab195046)

    FDFT1 was immunoprecipitated from HepG2 whole cell extract with ab195046 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab195046 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HepG2 whole cell extract (Input) 10 μg. Lane 2: ab195046 IP in HepG2 whole cell extract. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab195046 in HeLa whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-FDFT1 antibody [EPR16481] (ab195046)

    Predicted band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FDFT1 antibody [EPR16481] (ab195046), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FDFT1 antibody [EPR16481] (ab195046)

    Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma tissue sections labeling FDFT1 with ab195046 at a 1/16000 dilution. Goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FDFT1 antibody [EPR16481] (ab195046), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FDFT1 antibody [EPR16481] (ab195046)

    Immunohistochemical analysis of paraffin-embedded human sebaceous carcinoma tissue sections labeling FDFT1 with ab195046 at a 1/16000 dilution. Goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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