AB277132

Anti-FDPS/FPS antibody [005]

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal FDPS/FPS antibody. Carrier free. Suitable for ICC, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FDPS aa 1-400.

View Alternative Names

FPS, KIAA1293, FDPS, Farnesyl pyrophosphate synthase, FPP synthase, Dimethylallyltranstransferase, Farnesyl diphosphate synthase, Geranyltranstransferase

4 Images

Flow Cytometry (Intracellular) - Anti-FDPS/FPS antibody [005] (AB277132)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FDPS/FPS antibody [005] (AB277132)

Intracellular flow cytometric analysis of FDPS/FPS expression on HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were stained with ab277132 at 1/25 dilution, then a FITC-conjugated second step antibody. The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

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Immunocytochemistry - Anti-FDPS/FPS antibody [005] (AB277132)

Immunofluorescence staining of FDPS/FPS in HUVEC (human umbilical vein endothelial cell line) cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab277132 at 1/60 dilution at 37°C for 1 hour. Then cells were stained with an Alexa Fluor^® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

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Immunoprecipitation - Anti-FDPS/FPS antibody [005] (AB277132)

FDPS/FPS was immunoprecipitated from :

Lane A : 0.5 mg Hela (human epithelial cell line from cervix adenocarcinoma) Whole Cell Lysate
Lane B : 0.5 mg HepG2 (human liver hepatocellular carcinoma cell line) Whole Cell Lysate
Lane C : 0.5 mg A549 (human lung carcinoma cell line) Whole Cell Lysate

2 μL ab277132 and 15 μl of 50 % Protein G agarose.

Western blot was performed using ab277132, at 1/100 dilution

Secondary antibody : Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilution

Performed under reducing conditions.

All lanes:

Immunoprecipitation - Anti-FDPS/FPS antibody [005] (ab277132)

Predicted band size: 48 kDa

false

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Western blot - Anti-FDPS/FPS antibody [005] (AB277132)

Performed under reducing conditions.

All lanes:

Western blot - Anti-FDPS/FPS antibody [005] (ab277132) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

005

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, ICC, Flow Cyt (Intra)

applications

Immunogen

Recombinant Fragment Protein within Human FDPS aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

P14324

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/100", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/25 - 1/100", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Farnesyl diphosphate synthase (FDPS) also known as farnesyl pyrophosphate synthase (FPS) is an important enzyme that catalyzes the synthesis of farnesyl diphosphate (FPP) from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). This enzyme plays an important role in the mevalonate pathway which is essential for the biosynthesis of sterols including cholesterol and other isoprenoids. FDPS is a protein with a molecular mass of approximately 42 kDa. Its expression occurs in various tissues with significant levels in liver brain and bone where it contributes to different cellular metabolic processes.

Biological function summary

Farnesyl diphosphate synthase facilitates the production of FPP a common precursor for the synthesis of isoprenoid compounds such as cholesterol ubiquinones and dolichol. FDPS does not form a complex but works in coordination with other enzymes in the mevalonate pathway. Its activity supports numerous cellular functions including membrane assembly protein prenylation and cell signaling. The enzyme’s role is important for normal cellular growth and development influencing processes like cell differentiation and proliferation.

Pathways

FDPS plays a significant role in the cholesterol biosynthesis and protein prenylation pathways. These pathways are critical for cellular homeostasis and involve several enzymes including HMG-CoA reductase and squalene synthase. The cholesterol biosynthesis pathway includes multiple steps where FPP serves as a lipid intermediate. Meanwhile in protein prenylation FPP contributes to the post-translational modification of proteins such as small GTPases which impacts cell signaling and cytoskeletal organization.

FDPS has a connection to bone diseases such as osteoporosis and also links to cardiovascular disorders. The relationship to osteoporosis arises from its role in bone resorption and osteoclast activity mediated by proteins like RANKL and osteoprotegerin. Cardiovascular disorders may link to altered cholesterol metabolism with FDPS influencing cholesterol biosynthesis and regulation. Inhibition of FDPS activity targets these pathways leading to therapeutic strategies that may address both metabolic and bone health issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.

See full target information FDPS

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 12:7470-7480 PubMed34632937

2021

Upregulation of ubiquitin conjugating enzyme E2B (Ube2b) ameliorates neuropathic pain by regulating Kcna2 (potassium voltage-gated channel subfamily A member 2) in primary afferent neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanzhi Peng,Qingqing Zhang,Hao Cheng,Guizhen Yan,Chunli Xing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FDPS/FPS antibody [005]

Flow Cytometry (Intracellular) - Anti-FDPS/FPS antibody [005] (AB277132)

Immunocytochemistry - Anti-FDPS/FPS antibody [005] (AB277132)

Immunoprecipitation - Anti-FDPS/FPS antibody [005] (AB277132)

Western blot - Anti-FDPS/FPS antibody [005] (AB277132)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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