AB189874

Anti-FDPS/FPS antibody - C-terminal

Name: Anti-FDPS/FPS antibody - C-terminal (ab189874) | Abcam
Brand: Abcam Limited
SKU: AB189874
Price: 535 USD
Availability: InStock

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(8 Publications)

Rabbit Polyclonal FDPS/FPS antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FDPS aa 100-450.

View Alternative Names

FPS, KIAA1293, FDPS, Farnesyl pyrophosphate synthase, FPP synthase, Dimethylallyltranstransferase, Farnesyl diphosphate synthase, Geranyltranstransferase

1 Images

Supplier Data

Western blot - Anti-FDPS/FPS antibody - C-terminal (AB189874)

All lanes:

Western blot - Anti-FDPS/FPS antibody - C-terminal (ab189874) at 1/500 dilution

Lane 1:

293T cell line extract

Lane 2:

HepG2 cell line extract

Lane 3:

HeLa cell line extract

Lane 4:

MCF7 cell line extract

Lane 5:

U-251MG cell line extract

Lane 6:

BxPC3 cell line extract

Lane 7:

Mouse liver extract

Lane 8:

Mouse ovary extract

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FDPS aa 100-450. The exact immunogen used to generate this antibody is proprietary information.

P14324

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody." }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Farnesyl diphosphate synthase (FDPS) also known as farnesyl pyrophosphate synthase (FPS) is an important enzyme that catalyzes the synthesis of farnesyl diphosphate (FPP) from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). This enzyme plays an important role in the mevalonate pathway which is essential for the biosynthesis of sterols including cholesterol and other isoprenoids. FDPS is a protein with a molecular mass of approximately 42 kDa. Its expression occurs in various tissues with significant levels in liver brain and bone where it contributes to different cellular metabolic processes.

Biological function summary

Farnesyl diphosphate synthase facilitates the production of FPP a common precursor for the synthesis of isoprenoid compounds such as cholesterol ubiquinones and dolichol. FDPS does not form a complex but works in coordination with other enzymes in the mevalonate pathway. Its activity supports numerous cellular functions including membrane assembly protein prenylation and cell signaling. The enzyme’s role is important for normal cellular growth and development influencing processes like cell differentiation and proliferation.

Pathways

FDPS plays a significant role in the cholesterol biosynthesis and protein prenylation pathways. These pathways are critical for cellular homeostasis and involve several enzymes including HMG-CoA reductase and squalene synthase. The cholesterol biosynthesis pathway includes multiple steps where FPP serves as a lipid intermediate. Meanwhile in protein prenylation FPP contributes to the post-translational modification of proteins such as small GTPases which impacts cell signaling and cytoskeletal organization.

FDPS has a connection to bone diseases such as osteoporosis and also links to cardiovascular disorders. The relationship to osteoporosis arises from its role in bone resorption and osteoclast activity mediated by proteins like RANKL and osteoprotegerin. Cardiovascular disorders may link to altered cholesterol metabolism with FDPS influencing cholesterol biosynthesis and regulation. Inhibition of FDPS activity targets these pathways leading to therapeutic strategies that may address both metabolic and bone health issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.

See full target information FDPS

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Anticancer research 43:547-555 PubMed36697063

2023

Hypoxia-adapted Multiple Myeloma Stem Cells Resist γδ-T-Cell-mediated Killing by Modulating the Mevalonate Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Sano,Naoko Kuwabara,Saori Nakagawa,Yuki Toda,Shigekuni Hosogi,Shinji Sato,Eishi Ashihara

Nature communications 12:3005 PubMed34021134

2021

Qki activates Srebp2-mediated cholesterol biosynthesis for maintenance of eye lens transparency.

Applications

Unspecified application

Species

Unspecified reactive species

Seula Shin,Hao Zhou,Chenxi He,Yanjun Wei,Yunfei Wang,Takashi Shingu,Ailiang Zeng,Shaobo Wang,Xin Zhou,Hongtao Li,Qiang Zhang,Qinling Mo,Jiafu Long,Fei Lan,Yiwen Chen,Jian Hu

Molecular medicine reports 22:165-174 PubMed32319638

2020

MicroRNA‑125a‑mediated regulation of the mevalonate signaling pathway contributes to high glucose‑induced proliferation and migration of vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Ye,Guo-Hua Lou,Ai-Chun Li,Feng-Qin Dong,Guo-Ping Chen,Wei-Wei Xu,Yan-Ning Liu,Shen-Jiang Hu

International journal of molecular sciences 20: PubMed31561416

2019

Ursolic Acid Suppresses Cholesterol Biosynthesis and Exerts Anti-Cancer Effects in Hepatocellular Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Geon-Hee Kim,Sang-Yeon Kan,Hyeji Kang,Sujin Lee,Hyun Myung Ko,Ji Hyung Kim,Ji-Hong Lim

Cancers 11: PubMed31540279

2019

HSF1 Regulates Mevalonate and Cholesterol Biosynthesis Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeji Kang,Taerim Oh,Young Yil Bahk,Geon-Hee Kim,Sang-Yeon Kan,Dong Hoon Shin,Ji Hyung Kim,Ji-Hong Lim

International journal of molecular sciences 19: PubMed30321984

2018

Emodin Sensitizes Hepatocellular Carcinoma Cells to the Anti-Cancer Effect of Sorafenib through Suppression of Cholesterol Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Young-Seon Kim,Yoon-Mi Lee,Taek-In Oh,Dong Hoon Shin,Geon-Hee Kim,Sang-Yeon Kan,Hyeji Kang,Ji Hyung Kim,Byeong Mo Kim,Woo Jong Yim,Ji-Hong Lim

eLife 6: PubMed29132502

2017

Transcriptomic and proteomic landscape of mitochondrial dysfunction reveals secondary coenzyme Q deficiency in mammals.

Applications

Unspecified application

Species

Unspecified reactive species

Inge Kühl,Maria Miranda,Ilian Atanassov,Irina Kuznetsova,Yvonne Hinze,Arnaud Mourier,Aleksandra Filipovska,Nils-Göran Larsson

Molecular medicine reports 16:9593-9600 PubMed29039598

2017

Expression of key enzymes in the mevalonate pathway are altered in monocrotaline-induced pulmonary arterial hypertension in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Jiang,Yu Chen,Yuxiang Zhu,Guosheng Fu,Shiming Xu

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-FDPS/FPS antibody - C-terminal

Western blot - Anti-FDPS/FPS antibody - C-terminal (AB189874)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise