AB91650

Anti-FE65 antibody [EPR3538]

RabMAb
Recombinant
KO Validated
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(4 Publications)

Rabbit Recombinant Monoclonal FE65 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

FE65, RIR, APBB1, Amyloid beta precursor protein binding family B member 1, Amyloid-beta A4 precursor protein-binding family B member 1, Protein Fe65

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FE65 antibody [EPR3538] (AB91650)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FE65 antibody [EPR3538] (AB91650)

ab91650 at 1/50 dilution staining FE65 in formalin-fixed paraffin-embedded Human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

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Western blot - Anti-FE65 antibody [EPR3538] (AB91650)

All lanes:

Western blot - Anti-FE65 antibody [EPR3538] (ab91650) at 1/2000 dilution

All lanes:

SH-SY5Y cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 77 kDa

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Lab

Western blot - Anti-FE65 antibody [EPR3538] (AB91650)

False colour image of Western blot : Anti-FE65 antibody [EPR3538] staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab91650 was shown to bind specifically to FE65. A band was observed at 65/85 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in APBB1 knockout cell line ab267294 (knockout cell lysate ab257833). To generate this image, wild-type and APBB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-FE65 antibody [EPR3538] (ab91650) at 1/2000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

APBB1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human APBB1 (FE65) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-apbb1-fe65-knockout-hek-293t-cell-line-ab267294'>ab267294</a>)

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

A431 cell lysate at 20 µg

Predicted band size: 77 kDa

Observed band size: 65 kDa

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Carrier free

Anti-FE65 antibody [EPR3538] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3538

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "The use of an HRP/AP polymerized antibody is recommended. We have compared both the HRP-conjugated and the polymerized HRP and found stronger signals can be obtained with the polymerized antibody.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FE65 also known as APBB1 is a protein with a molecular mass of approximately 65 kDa. It belongs to the family of adaptor proteins with important roles in different cellular processes. This protein is expressed abundantly in the brain particularly in neurons where it interacts with other proteins to mediate signaling pathways. FE65 contains several conserved domains including a WW domain and two phosphotyrosine-binding (PTB) domains which facilitate its interaction with multiple partners.

Biological function summary

FE65 plays a significant role in neuronal development and cognitive functions. It is part of a protein complex that includes the amyloid precursor protein (APP). This complex is important for the regulation of gene expression and cellular communication. FE65 also affects the nuclear signaling by interacting with the chromatin-remodeling complex. These interactions suggest that FE65 has a hand in influencing transcriptional activities within the cell.

Pathways

FE65 is involved in the APP processing pathway and the development of Alzheimer's disease. It interacts with APP and influences its role in the cleavage and formation of amyloid-beta peptides. These peptides are key factors within the Alzheimer's disease pathway. Moreover FE65 connects with other proteins such as Abl which is involved in cell differentiation and migration processes. Through these pathways FE65 contributes to a broad range of neuronal functions and activities.

FE65 has a close connection to Alzheimer’s disease and cognitive impairments. In Alzheimer's disease FE65 interacts with APP modulating the production of amyloid-beta an important pathological component of the disease. The accumulation of amyloid-beta is linked to the progression of symptoms in Alzheimer’s patients. Additionally FE65 is associated with other proteins related to neuronal dysfunction and synaptic plasticity which may impact cognitive disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription coregulator that can have both coactivator and corepressor functions (PubMed : 15031292, PubMed : 18468999, PubMed : 18922798, PubMed : 25342469, PubMed : 33938178). Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain (PubMed : 15031292, PubMed : 18468999, PubMed : 18922798, PubMed : 25342469). Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis (PubMed : 15031292, PubMed : 18468999, PubMed : 18922798, PubMed : 25342469). May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1 (PubMed : 19234442). Required for histone H4 acetylation at double-strand breaks (DSBs) (PubMed : 19234442). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity (PubMed : 33938178). Functions in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4 (PubMed : 19343227). Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s) (PubMed : 19343227). Involved in hippocampal neurite branching and neuromuscular junction formation, as a result plays a role in spatial memory functioning (By similarity). Plays a role in the maintenance of lens transparency (By similarity). May play a role in muscle cell strength (By similarity). Acts as a molecular adapter that functions in neurite outgrowth by activating the RAC1-ARF6 axis upon insulin treatment (PubMed : 36250347).

See full target information APBB1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 218: PubMed34156424

2021

GSAP regulates lipid homeostasis and mitochondrial function associated with Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Xu,Jerry C Chang,Xiaopu Zhou,Wei Wang,Michael Bamkole,Eitan Wong,Karima Bettayeb,Lu-Lin Jiang,Timothy Huang,Wenjie Luo,Huaxi Xu,Angus C Nairn,Marc Flajolet,Nancy Y Ip,Yue-Ming Li,Paul Greengard

Cells 9: PubMed32751526

2020

Fyn Tyrosine Kinase Elicits Amyloid Precursor Protein Tyr682 Phosphorylation in Neurons from Alzheimer's Disease Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Filomena Iannuzzi,Rossana Sirabella,Nadia Canu,Thorsten J Maier,Lucio Annunziato,Carmela Matrone

Journal of neurochemistry 149:399-412 PubMed30664241

2019

Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms.

Applications

Unspecified application

Species

Unspecified reactive species

Robert J Andrew,Kate Fisher,Kate J Heesom,Katherine A B Kellett,Nigel M Hooper

PloS one 11:e0155056 PubMed27176072

2016

Fe65 Is Phosphorylated on Ser289 after UV-Induced DNA Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah Langlands,Peter G Blain,Paul A Jowsey

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FE65 antibody [EPR3538]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FE65 antibody [EPR3538] (AB91650)

Western blot - Anti-FE65 antibody [EPR3538] (AB91650)

Western blot - Anti-FE65 antibody [EPR3538] (AB91650)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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