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Rabbit Recombinant Monoclonal FE65 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

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Images

Western blot - Anti-FE65 antibody [EPR3538] (AB91650), expandable thumbnail
  • Western blot - Anti-FE65 antibody [EPR3538] (AB91650), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FE65 antibody [EPR3538] (AB91650), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWB
Human
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

The use of an HRP/AP polymerized antibody is recommended. We have compared both the HRP-conjugated and the polymerized HRP and found stronger signals can be obtained with the polymerized antibody.

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000 - 1/5000
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Transcription coregulator that can have both coactivator and corepressor functions (PubMed:15031292, PubMed:18468999, PubMed:18922798, PubMed:25342469, PubMed:33938178). Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain (PubMed:15031292, PubMed:18468999, PubMed:18922798, PubMed:25342469). Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis (PubMed:15031292, PubMed:18468999, PubMed:18922798, PubMed:25342469). May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1 (PubMed:19234442). Required for histone H4 acetylation at double-strand breaks (DSBs) (PubMed:19234442). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity (PubMed:33938178). Functions in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4 (PubMed:19343227). Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s) (PubMed:19343227). Involved in hippocampal neurite branching and neuromuscular junction formation, as a result plays a role in spatial memory functioning (By similarity). Plays a role in the maintenance of lens transparency (By similarity). May play a role in muscle cell strength (By similarity). Acts as a molecular adapter that functions in neurite outgrowth by activating the RAC1-ARF6 axis upon insulin treatment (PubMed:36250347).

Alternative names

FE65, RIR, APBB1, Amyloid beta precursor protein binding family B member 1, Amyloid-beta A4 precursor protein-binding family B member 1, Protein Fe65

Recommended products

Rabbit Recombinant Monoclonal FE65 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3538
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FE65 also known as APBB1 is a protein with a molecular mass of approximately 65 kDa. It belongs to the family of adaptor proteins with important roles in different cellular processes. This protein is expressed abundantly in the brain particularly in neurons where it interacts with other proteins to mediate signaling pathways. FE65 contains several conserved domains including a WW domain and two phosphotyrosine-binding (PTB) domains which facilitate its interaction with multiple partners.

Biological function summary

FE65 plays a significant role in neuronal development and cognitive functions. It is part of a protein complex that includes the amyloid precursor protein (APP). This complex is important for the regulation of gene expression and cellular communication. FE65 also affects the nuclear signaling by interacting with the chromatin-remodeling complex. These interactions suggest that FE65 has a hand in influencing transcriptional activities within the cell.

Pathways

FE65 is involved in the APP processing pathway and the development of Alzheimer's disease. It interacts with APP and influences its role in the cleavage and formation of amyloid-beta peptides. These peptides are key factors within the Alzheimer's disease pathway. Moreover FE65 connects with other proteins such as Abl which is involved in cell differentiation and migration processes. Through these pathways FE65 contributes to a broad range of neuronal functions and activities.

Associated diseases and disorders

FE65 has a close connection to Alzheimer’s disease and cognitive impairments. In Alzheimer's disease FE65 interacts with APP modulating the production of amyloid-beta an important pathological component of the disease. The accumulation of amyloid-beta is linked to the progression of symptoms in Alzheimer’s patients. Additionally FE65 is associated with other proteins related to neuronal dysfunction and synaptic plasticity which may impact cognitive disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-FE65 antibody [EPR3538] (ab91650), expandable thumbnail

    Western blot - Anti-FE65 antibody [EPR3538] (ab91650)

    False colour image of Western blot: Anti-FE65 antibody [EPR3538] staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab91650 was shown to bind specifically to FE65. A band was observed at 65/85 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in APBB1 knockout cell line Human APBB1 (FE65) knockout HEK-293T cell line ab267294 (knockout cell lysate Human APBB1 (FE65) knockout HEK-293T cell lysate ab257833). To generate this image, wild-type and APBB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-FE65 antibody [EPR3538] (ab91650) at 1/2000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: APBB1 knockout HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human APBB1 (FE65) knockout HEK-293T cell line (Human APBB1 (FE65) knockout HEK-293T cell line ab267294)

    Lane 3: SH-SY5Y cell lysate at 20 µg

    Lane 4: A431 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 77 kDa

    Observed band size: 65 kDa

  • Western blot - Anti-FE65 antibody [EPR3538] (ab91650), expandable thumbnail

    Western blot - Anti-FE65 antibody [EPR3538] (ab91650)

    All lanes: Western blot - Anti-FE65 antibody [EPR3538] (ab91650) at 1/2000 dilution

    All lanes: SH-SY5Y cell lysates at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 77 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FE65 antibody [EPR3538] (ab91650), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FE65 antibody [EPR3538] (ab91650)

    ab91650, at 1/50 dilution, staining FE65 in formalin-fixed, paraffin-embedded Human brain tissue, by Immunohistochemistry.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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